Induction and morphological and biochemical characterization of Hyptis leucocephala (Lamiaceae) calluses

Danilo Marcelo Santos Pereira, Lenaldo Muniz de Oliveira, Cristina Ferreira Nepomuceno, José Raniere Ferreira de Santana, Marília Lordelo Cardoso Silva, Maria Gabriela Bello Koblitz

Abstract


Hyptis leucocephala is an aromatic herb endemic to the Brazilian semiarid region, with antimicrobial, antifungal,
cytotoxic, anti-inflammatory and anti-HIV properties. In vitro cultivation makes the production of high genetic and
sanitary quality cuttings possible for this species, allowing its sustainable exploitation. In the present study, the effect of 6-
benzilaminopurine (BAP) and naftalenoacetic acid (NAA) on callus induction in leaf segments of this species and
different organic additives to WPM (Wood Plant Medium) for the callus induction was evaluated. The combination of
BAP and NAA allowed the induction of compact and undifferentiated calluses. The highest rate of callus formation and
growth was obtained with the combination of 8.88 μM BAP and 21.48 μM NAA. Supplementing the culture medium with
coconut water, malt extract and yeast extract did not induce friable calluses. The levels of total sugars, reducing sugars,
protein, and amino acids were reduced in calluses maintained in culture medium supplemented with coconut water, malt
extract and yeast extract.



DOI: http://dx.doi.org/10.13102/scb124

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