Organogenesis in three species of Annona (Annonaceae)

José Raniere Ferreira de Santana, Renato Paiva, Lenaldo Muniz de Oliveira, Cristina Ferreira Nepomuceno, Daniela Garcia Silveira

Abstract


This study aimed to evaluate, under in vitro conditions, the regenerative potential of leaves of three species of
Annona (Annonaceae), A. squamosa, A. bahiensis and A. glabra, and hypocotyl and epicotyl segments of A. squamosa.
In the first study, organogenesis was induced by inoculating the first leaves of three species of Annona in WPM medium
supplemented with all combinations of concentrations of BAP (2.22, 4.44, 8.88 and 17.76 μM) and NAA (0.00, 1.34, 2.68
and 5.37 μM). The cotyledons and first leaves of A. squamosa were also cultivated in the same culture media. In the
second study, hypocotyl and epicotyl segments of A. squamosa were inoculated in a medium supplemented with different
concentrations of BAP (0.00, 2.22, 8.87, 17.74 and 35.48 μM). The results showed that organogenesis in Annona leaves
is dependent on the type of explant, genotype and auxin-cytokinin balance used. In A. squamosa, the cotyledons had
higher organogenic potential for micropropagation. Multiple shoots were regenerated from A. squamosa hypocotyls and
epicotyls, but the regenerative potential of the hypocotyl was higher than that of the epicotyl.

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DOI: http://dx.doi.org/10.13102/scb85

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