Effects of Diafenthiuron in Toxic Baits on Colonies of Leaf-cutting ants (Hymenoptera: Formicidae)

Marcílio Souza Barbosa, Luiz Carlos Forti, Ricardo Toshio Fujihara, Carlos Gilberto Raetano

Abstract


Dianfenthiuron is a pre-insecticide that can be activated by photolysis, and may be a promising formicide. This study evaluated the effect of diafenthiuron after photolysis in colonies of Atta sexdens rubropilosa Forel, 1908. The experiment was conducted in a completely randomized design with five treatments and five replications: control (no active ingredient), sulfluramid (standard formicide), diafenthiuron (no exposure to UV), diafenthiuron (2h exposure to UV) and diafenthiuron (6h exposure to UV). Toxic baits were applied at a rate of 0.5 g per colony, and we observed the transport and incorporation of the baits into the colonies. A grading scale was used (0 to 4) to measure the cutting of Acalypha L. (Euphorbiaceae) leaves by workers at 2, 7, 14 and 21 days after application (DAA) and we also measured the garden mass (fungus + adult + brood) at 21 DAA in order to check for growth of the fungus culture. Total loading and incorporation occurred one hour after application of the baits. Colonies that received sulfluramid did not transport leaves at 2 DAA. Workers that received baits with D2h showed an average of 20% transport and 55% incorporation of leaves at 21 DAA. The grading scale indicated that treatments D2h and D6h had the lowest averages, 0.80 and 2.00, respectively. The treatments D2h and D6h reduced cutting of leaves and fungus garden mass, but did not kill the colonies of A. sexdens rubropilosa.


Keywords


Atta; chemical control; ultraviolet light; carbodiimide

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References


Brugger, M. S., Fernandes, M. A. C., Hallack, N. M. R. & Lopes, J. F. S. (2008). Avaliação dos efeitos tóxicos de extrato hexânico de Azadirachta indica (A. Juss) em colônias de Acromyrmex rugosus (Smith, 1858) (Formicidae, Attini). Revista Brasileira de Zoociências, 10: 233-238.

Bueno, F. C. Seleção de ingredientes ativos para o desenvolvimento de iscas tóxicas para o controle de formigas-cortadeiras (Hymenoptera: Formicidae). 2013. Tese (Doutorado em Ciências Biológicas) – Botucatu-SP, Universidade Estadual Paulista, 75p.

Dekeyser, M. A. (2005). Acaricide mode of action. Pest Management Science, 61: 103-110. doi: 10.1002/ps.994

Delabie, J.H.C., Della Lucia, T. & Pastre, L. (2000). Protocolo de experimentação para avaliar a atratividade de novas formulações de iscas granuladas utilizadas no controle das formigas cortadeiras Acromyrmex spp. e Atta spp. (Hymenoptera: Formicidae: Myrmicinae: Attini) no campo. Anais da Sociedade Entomológica do Brasil, 29: 843-848. doi: 10.1590/S0301-80592000000400029

Ferreira, D.F. (2011). Sisvar: um sistema computacional de análise estatística. Ciência e Agrotecnologia, 35: 1039-1042. doi: 10.1590/S1413-70542011000600001

Forti, L.C., Nagamoto, N.S., Ramos, V.M., Andrade, A.P.P., Lopes, J.F.S., Camargo, R.S., Moreira, A.A. & Boaretto, M.A.C. (2003). Eficiencia de sulfluramida, fipronil y clorpirifoscomo sebos en el control de Atta capiguara Gonçalves (Hymenoptera: Formicidae). Pasturas Tropicales, 25: 28-35.

Forti, L.C., Pretto, D.R., Nagamoto, N.S., Padovani, C.R., Camargo, R.S. & Andrade, A.P.P. (2007). Dispersal of the delayed action insecticide sulfluramid in colonies of the leaf-cutting ant Atta sexdens rubropilosa (Hymenoptera: Formicidae). Sociobiology, 50: 1149-1163.

Heong, K.L., Tan, K.H., Garcia, C.P.F., Fabellar, L.T. & Lu, Z. (2011). Research methods in toxicology and insecticide resistance monitoring of rice planthoppers. Los Baños (Philippines): International Rice Research Institute, 101p.

Ishaaya, I., Barazani, A., Kontsedalov, S. & Horowitz, A.R. (2007). Insecticides with novel modes of action: mechanism, selectivity and cross-resistance. Entomological Research, 37: 148-152. doi: 10.1111/j.1748-5967.2007.00104.x

Kayser, H. & Eilinger, P. (2001). Metabolism of diafenthiuron by microsomal oxidation: procide activation and inactivation as mechanisms contributing to selectivity. Pest Management Science, 57: 975-980. doi: 10.1002/ps.360

Keum, Y-S., Kim, J.H., Kim, Y.W., Kim, K. & Li, Q.X. (2002). Photodegradation of diafenthiuron in water. Pest Management Science, 58: 496-502. doi: 10.1002/ps.483

Nagamoto, N.S., Forti, L.C. & Raetano, C.G. (2007). Evaluation of the adequacy of diflubenzuron and dechlorane in toxic baits for leaf-cutting ants (Hymenoptera: Formicidae) based on formicidal activity. Journal of Pest Science, 80: 9-13. doi: 10.1007/s10340-006-0143-8

Nagamoto, N.S., Forti, L.C., Andrade, A.P.P., Boaretto, M.A.C. & Wilcken, C.F. (2004). Method for the evaluation of insecticidal activity over time in Atta sexdens rubropilosa workers (Hymenoptera: Formicidae). Sociobiology, 44: 413-431.

Preetha, G., Stanley, J., Manoharan, T., Chandrasekaran, S. & Kutallam, S. (2009). Toxicity of imidacloprid and diafenthiuron to Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) in the laboratory conditions. Journal of Plant Protection Research, 49: 290-296. doi: 10.2478/v10045-009-0047-8.

Reis, M.A., Zanetti, R., Scolforo, J.R.S. & Ferreira, M.Z. (2010). Amostragem de formigas-cortadeiras (Hymenoptera: Formicidae) em eucaliptais pelos métodos de transectos em faixa e em linha. Revista Árvore, 34: 1101-1108. doi: 10.1590/S0100-67622010000600016

Ruder, F.J. & Kayser, H. (1993). The carbodiimide product of diafenthiuron inhibts mitochondria in vivo. Pesticide Biochemistry and Physiology, 46: 96-106. doi: 10.1006/pest.1993.1041

Zanetti, R., Zanuncio, J.C., Souza-Silva, A. & Abreu, L.G. (2003). Eficiência de isca formicida aplicada sobre o monte de terra solta de ninhos de Atta sexdens rubropilosa (Hymenoptera: Formicidae). Revista Árvore, 27: 410-407. doi: 10.1590/S0100-67622003000300019




DOI: http://dx.doi.org/10.13102/sociobiology.v64i3.1070

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