Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites

Yu-Lei Dang, Hong-Gui Zhang, Yu-Feng Meng, Min Zhang, Sha Zhao, Ping You, Xiao-Hong Su, Lian-Xi Xing

Abstract


We isolated 15 and 18 highly polymorphic genomic microsatellite markers from two subterranean termites, Reticulitermes aculabialis and R. labralis, respectively. A total of 53 alleles were detected in 15 microsatellite loci of R. aculabialis, and the alleles were 3.533±1.302 (mean±SD), while the corresponding data of R. labralis were 115 detected alleles in 18 microsatellite loci with 6.389±1.754 alleles. The observed and expected heterozygosity was 0.496±0.236 and 0.564±0.125 in R. aculabialis, and 0.368±0.263 and 0.702±0.115 in R. labralis, respectively. Seven loci were highly polymorphic (PIC>0.5) in R. aculabialis, and 15 loci were highly polymorphic (PIC>0.5) in R. labralis. All loci showed Hardy–Weinberg equilibrium. These polymorphic markers provide useful tools for population genetic and breeding system studies of subterranean termites.


Keywords


Subterranean termites; Reticulitermes; Microsatellite loci; Genetic diversity

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References


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DOI: http://dx.doi.org/10.13102/sociobiology.v64i3.1692

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