Floral Origin and Physical and Chemical Characteristics of Honey from Africanized Bees in Apiaries of Ubiratã and Nova Aurora, State of Paraná

Elizabete Satsuki Sekine, Eliza H. Takashiba, Raquel Oliveira Bueno, Paulo Agenor Alves Bueno, Marcelo G. Caxambu, Maria Josiane Sereia, Luís C. Marchini, Augusta Carolina C.C. Moreti, Vagner A.A. Toledo

Abstract


Physical and chemical characteristics of honey may vary due to the diversity of flora and soil characteristics, or seasonal factors. This study was carried out in two counties, Nova Aurora and Ubiratã, located in the West and Center-West regions of the State of Paraná. The objective of the study was to verify if the physical and chemical parameters of Apis mellifera (L.) honey are in accordance with the national standard, as well as to verify how the 21 samples collected in the two localities are grouped, based on the physical, chemical and pollen characteristics. Honey was analyzed for sugar, ash, protein, moisture, color, electrical conductivity, formaldehyde index, diastase and viscosity. Samples of honey containing the dominant pollen types Glycine max (L.) Merr. and Eucalyptus sp. formed groupings similar to those based on physical and chemical characteristics, however, the multivariate classification of honey samples in groups based on pollen types was not an efficient method to group samples of polyfloral honey.

Keywords


Apis mellifera; physical and chemical parameters; honey composition.

Full Text:

PDF

References


Aganin, A.F. (1971). Electrical conductivity of several unifloral honeys. Trudy Saratovskogo Zootekhnicheskogo Instituta, 21: 137-144.

Almeida -Anacleto, D. & Marchini L.C. (2004). Composição físico-química de amostras de méis Apis mellifera L. provenientes do cerrado paulista. Boletim de Indústria Animal, 61: 161-172.

Almeida-Muradian, L.B., Sousa, R.J, Barth, O.M., Gallmann, P. (2014). Preliminary data on brazilian monofloral honey from the northeast region using FT IR ATR spectroscopic, palynological, and color analysis. Quimica Nova, 37: 716-719.

Alves, E.M., Sereia, M.J., Toledo, V.A.A., Marchini, L.C., Neves, C.A., Toledo, T.C.S.O.A, Almeida-Anacleto, D. (2011). Physicochemical characteristics of organic honey samples of africanized honeybees from Parana River islands. Ciência e Tecnologia de Alimentos, 31: 635-639.

Araujo, D.F.D., Moreti, A.C.C.C; Silveira, T.A., Marchini, L.C. & Otsuk, I.P. (2013). Pollen Content in Honey of Apis mellifera Linnaeus (Hymenoptera, Apidae) in an Atlantic Forest Fragment in the Municipality of Piracicaba, São Paulo State, Brazil. Sociobiology, 60: 436-440. doi: 10.13102/sociobiology.v60i4.436-440

Arruda, C.M.F., Marchini, L.C., Moreti, A.C., Otsuk, I.P. & Sodré, G.S. (2005). Características físico-químicas de méis da Chapada do Araripe/Santana do Cariri-Ceará. Acta Scientiarum Animal Sciences, 27: 171-176.

Atago Co LTDA. (1988). Refratômetro para mel. Abelhas, 31: 9-44.

Barth, O.M. (1989). O pólen no mel brasileiro. Rio de Janeiro: Luxor, 150p.

Barth, O.M. (2004). Melissopalynology in Brazil: a review of pollen analysis of honeys, própolis and pollen loads of bees. Scientia Agricola, 61: 342-350.

Barth, O.M., Freitas, A.S., Sousa, G.L., Almeida-Muradian, L.G. (2013). Pollen And physicochemical analysis of Apis and Tetragonisca (Apidae) honey. Interciencia, 38: 280-285.

Barth, O.M., Maiorino, C., Benatti, A.P.T. & Bastos, D.H.M. (2005). Determinação de parâmetros físico-químicos e da origem botânica de méis indicados monoflorais do sudeste do Brasil. Ciência e Tecnologia de Alimentos, 25: 229-233.

Bastos, E.M.A.F., Silveira, V.M. & Soares, A.E.E. (2003). Pollen spectrum of honey produced in Cerrado areas of Minas Gerais state. Brazilian Journal of Biology, 63: 599-615.

Bath, P.K. & Singh, N. (1999). A comparison between Helianthus annuus and Eucalyptus lanceolatus honey. Food Chemistry, 67: 389-397.

Boe. (1986). Orden de 12 de junio de 1986, de la Presidencia del Gobierno por la que se aprueban los métodos oficiales de análisis para la miel. Boletin Oficial Español, 145: 22195-22202.

Boffo, E.F., Tavares, L.A., Tobias, A.C.T., Ferreira, M., Ferreira, A.G. (2012). Identification of componentes of Brazilian honey by H NMR and classification of its botanical origin by chemometric methods. Food Science and Technology, 49: 55-63

Bogdanov, S. (1999). Honey quality and internacional regulatory standards: review by the International Honey Commission. Bee world, 80: 61-69.

Bosco, L.B. & Luz, C.F.P. (2017). Pollen analysis of Atlantic forest honey from the Vale do Ribeira Region, state of São Paulo, Brazil. Grana, 57: 144-157. doi: 10.10 80/001731 34.2017.1319414

Brasil - Ministério da Agricultura, Pecuária e Abastecimento. (2000). Instrução Normativa 11, de 20 de outubro de 2000, Regulamento Técnico de identidade e qualidade do mel. Available at: http://www.agricultura.gov.br/sda/dipoa/anexo_intrnorm11.htm.

CAC - Codex Alimentarius Commission. (1990). Official methods of analysis. Rome. V.3, supl.2, p.15-39.

Crane, E. (1990). Bees and beekeeping: science, practice and world resources. Oxford: Heinemann Newnes, 614p.

De-Melo, A.A.M., Almeida-Muradian, L.B., Sancho, M.T. & Pascual-Maté, A. (2017). Composition and properties of Apis mellifera honey: A review. Journal of Apicultural Research, 57: 5-37.

Erdtman, G. (1952). Pollen morphology and plant taxonomy- Angiosperms. Stockholm: Almqvist e Wiksel, 539 p.

Faria, J.A.F. (1993). Shelf life testing of honey. Ciência e Tecnologia de Alimentos, 13: 58-66.

Fattori, S.B. (2004). La miel: propiedades, composición y análisis físico-químico [Honey: Properties, composition and physicochemical analysis]. Buenos Aires: Comisión Apimondia “Tecnología y Productos”.

Finola, M.S., Lasagno, M.C. & Marioli, J.M. (2007). Microbiological and chemical characterization of honeys from central Argentina. Food Chemistry, 100: 1649-1653.

Ibge - Instituto Brasileiro de Geografia e Estatística. (2012). Manual Técnico da Vegetação Brasileira: Sistema fitogeográfico; Inventário das formações florestais e campestres; Técnicas e manejo de coleções botânicas; Procedimentos para mapeamentos. Manuais Técnicos em Geociências, n 1. 2 ed. Rio de Janeiro. 275p

Legendre, L. & Legendre, P. (1984). Ecologie numérique. 2. ed. Paris: Masson, 335p.

Louveaux, J., Maurizio, A. & Vorwohl, G. (1978). Methods of melissopalynology. Bee World, 59: 139-157.

Luz, C.F.P., Thomé, M.L. & Barth, O.M. (2007). Recursos tróficos de Apis mellifera L. (Hymenoptera, Apidae) na região de MorroAzul do Tinguá, Estado do Rio de Janeiro. Revista Brasileira de Botânica, 30: 29-36,

Malacalza, N.H., Mouteira, M.C., Baldi, B. & Lupano, C.E. (2007). Characterisation of honey from different regions of the Province of Bueno Aires, Argentina. Journal of Apicultural Research, 46: 8-14.

Manzanares, A.B., García, Z.H., Galdón, B.R., Rodríguez-Rodríguez, E.M. & Romero, C.D. (2017). Physicochemical characteristics and pollen spectrum of monofloral hoys from Tenerife, Spain. Food Chemistry, 228: 441-446. doi: 10.10 16/j.foodchem.2017.01.150

Marchini, L.C., Moreti, A.C. & Otsuk, I.P. (2005). Análise de agrupamento, com base na composição físico-química de amostras de méis produzidos por Apis mellifera L. do Estado de São Paulo. Ciência e Tecnologia de Alimentos, 25: 8-17.

Marchini, L.C., Moretti, A.C., Otsuki I.P. & Sodré, G. (2007). Physicochemical composition of Apis mellifera honey samples from São Paulo state, Brazil. Quimica Nova, 30: 1653-1657.

Marchini, L.C., Sodré, G.S. & Moreti, A.C. (2004a). Mel brasileiro: composição e normas. Ribeirão Preto: A.S. Pinto, 111p.

Marchini, L.C., Sodré, G.S., Moreti, A.C. & Otsuk, I.P. (2004b). Composição físico-química de amostras de méis de Apis mellifera L. do Estado de Tocantis, Brasil. Boletim de Indústria Animal, 61: 101-114.

Mendonça, K., Marchini, L.C., Souza, B.A., Almeida-Anacleto, D. & Moreti, A.C. (2008). Caracterização físico-química de amostras de méis produzidas por Apis mellifera L. em fragmento de cerrado no município de Itirapina, São Paulo. Ciência Rural, 38: 1748-1753.

Moraes, R.M. & Teixeira, E.W. (1998). Analise de Mel (Manual Técnico). Pindamonhangaba: Centro de apicultura tropical, IZ/SAA, 41p.

Mueller-Dumbois, D. & Ellenberg, H. (1974). Aims and methods of vegetation ecology, New York: John Willey, 547p.

Nogueira Couto, R.H. & Couto L.A. (2006). Apicultura: manejo e produtos. 3 ed., Jaboticabal: FUNEP, 193 p.

Nozal, M.J., Bernal, J.L., Toribio, L., Alamo, M., Diego, J.C., & Tapia, J. (2005). The use of carbohydrate profiles and chemometrics in the characterization of natural honeys of identical geographical origin. Journal of Agricultural and Food Chemistry, 53: 3095-3100.

Oksanen, J., Blanchet, F.G., Friendly, M., Kindt, R., Legendre, P., Mcglinn, D., Minchin, P.R., O’hara, R.B., Simpson, G.L., Solymos, P., Stevens, M.H.H., Szoecs, E. & Wagner, H. (2017). Vegan: community ecology package. R package version, 2.4-2. Available at: https://CRAN.R-project.org/package=vegan. (Accessed 02 April 2018).

Pielou, E.C. (1975). Ecological diversity. New York: John Willey, 165p.

R Core Team. (2016). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available at: https://www.R-project.org/.

Rebane, R., Herodes, K. (2008). Evaluation of the Botanical Origin of Estonian Uni and Polyfloral Honeys by Amino Acid Content. Journal of Agricultural and Food Chemistry, 56: 10716–10720.

Santos, J. S., Santos, N.S., Santos, M.L.P., Santos, S.N. and Lacerda, J.J.J. Honey Classification from Semi-Arid, Atlantic and Transitional Forest Zones in Bahia, Brazil. Journal of the Brazilian Chemical Society, 2008(19): 502-508.

Sanz, S., Gradillas, G., Jimeno, F., Perez, C. & Juna, T. (1995). Fermentation Problem in Spanish North-Coast Honey. Journal of Food Protection, 58: 515-518.

Sekine, E.S.; Toledo, V.A.A.; Caxambu, M.G.; Chmura, S. Takashiba, E.H.; Sereia, M.J.; Marchini, L.C.; Moreti, A.C.C.C. (2013). Melliferous flora and pollen characterization of honey samples of Apis mellifera L., 1758 in apiaries in the counties of Ubiratã and Nova Aurora, PR. Anais da Academia Brasileira de Ciências. 85: 307-326. doi: 10.1590/S0001-37652013005000017

Sereia, J.A., Alves, E.M., Toledo, V.A.A., Marchini, L.C., Sekine, E.S., Faquinello, P., Almeida, D. & Moreti, A.C. (2011). Physicochemical characteristics of organic honeys of africanized honeybees from Paraná River islands. Anais da Academia Brasileira de Ciências 83: 1077-1090. doi: 10.1590/S0001-37652011000300026.

Silva, A.S., Alves, C.N., Fernandes, K.G., Müllerd, R.C.S. (2013). Classification of honeys from Pará state (Amazon region, Brazil) produced by three different species of bees using chemometric methods. Journal of the Brazilian Chemical Society, 24: 1135-1145.

Silveira, T.A., Correia-oliveira, M.E, Moreti, A.C.C.C., Otsuk, I.P. & Marchini, L.C. (2012). Botanical Origin of Protein Sources Used by Honeybees (Apis mellifera) in an Atlantic Forest. Sociobiology, 59: 1-10

Sodré, G.S., Marchini, L.C., Moreti, A.C. & Carvalho, C.A.L. (2003). Análises multivariadas com base nas características físico-químicas de amostras de méis de Apis mellifera L. (Hymenoptera: Apidae) da região litoral norte no estado da Bahia. Archivos Latinoamericanos de Producción Animal, 11: 129 -137.

Sodré, G.S., Marchini, L.C., Moreti, A.C. Otsuk, I.P. & Carvalho, C.A.L. (2007). Caracterização físico-química de amostras de méis de Apis mellifera L. (Hymenoptera: Apidae) do Estado do Ceará. Ciência Rural, 37: 1139-1144.

Terrab, A., González, A.G., Díez, M.J., Heredia, F.J., (2003). Characterisation of Moroccan unifloral honeys using multivariate analysis. European Food Research and Technology, 218: 88-95.

Vidal, R. & Fregosi, E.V. (1984). Mel: características, análises físico-químicas, adulteração e transformação. Barretos: Instituto Tecnológico Científico Roberto Rios.

White Jr, J.W. (1975). Physical characteristics of honey. In CRANEE. Honey a comprehensive survey, London: Heinemann, p.207–239.

White Jr, J.W. (1994). The role of hmf and diastase assays in honey quality evaluation. Bee World, 75: 104-117.

Zenebon, O., Pascuet, N.S. & Tiglea, P. (2008). Procedimentos e determinações gerais. In: Métodos físico-químicos para análise de alimentos. 4 ed. São Paulo: Instituto Adolfo Lutz, Cap. 4, p. 125-130.




DOI: http://dx.doi.org/10.13102/sociobiology.v66i1.3385

Refbacks

  • There are currently no refbacks.


JCR Impact Factor 2018: 0.604