Toxicity of Fenpyroximate, Difenoconazole and Mineral Oil on Apis mellifera L. Introduction

Authors

  • Delzuite Teles Leite Universidade Federal do Recôncavo da Bahia
  • Roberto Barbosa Sampaio Universidade Federal do Recôncavo da Bahia
  • Claudia Oliveira dos Santos Universidade Federal do Recôncavo da Bahia
  • Juliana Nascimento dos Santos Universidade Federal do Recôncavo da Bahia
  • Emerson Dechechi Chambó Universidade Federal do Amazonas
  • Carlos Alfredo Lopes de Carvalho Universidade Federal do Recôncavo da Bahia
  • Geni da Silva Sodré universidade Federal do Recôncavo da Bahia

DOI:

https://doi.org/10.13102/sociobiology.v65i4.3416

Keywords:

Synergism, beekeeping, useful insects

Abstract

Bees of genus Apis are the main crop pollinators; however, the use of pesticides in agriculture may intoxicate them during foraging. In this study, we evaluated the toxic eff ects caused by difenoconazole (fungicide), fenpyroximate (acaricide) and mineral oil (adjuvant) used alone and associated (pesticide + adjuvant) on workers of Apis mellifera L. Bees were exposed to product doses recommended by manufacturers, orally and in contact on a contaminated surface in a controlled environment. All products presented low lethality, both in isolation and combination (except for difenoconazole via contact), however, they all showed toxic effects. The results showed that combination of pesticides with adjuvant augmented toxic eff ects.

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References

Andrade Junior, E.R. & Galbieri, R. (2014). Efi ciência de fungicidas no controle de mancha de ramulária em algodoeiro, na safra 2013/14 no Mato Grosso. Circular técnica n, 12. Retrieved from: http://www.imamt.com.br/system/anexos/arquivos/260/original/circular_tecnica_edicao12_bx.pdf?1418381257

Balardin, R. 2015. Fungicidas sistêmicos: benzimidazóis, triazóis e estrobilurinas. https://phytusclub.com/materiaisdidaticos/fungicidassistemicos-benzimidazois-triazois-eestrobilurinas/. (accessed date: 18 July, 2018).

Blacquiere, T., Smagghe, G., Van Gestel, C.A. & Mommaerts, V. (2012). Neonicotinoids in bees: a review on concentrations, side-effects and risk assessment. Ecotoxicology, 21: 973-992. doi: 10.1007/s10646-012-0863-x

Brandt, A., Gorenfl o, A., Siede, R., Meixner, M. & Büchler, R. (2016). The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.). Journal of Insect Physiology, 86: 40-47. doi: 10.1016/j.jinsphys.2016.01.001

Carvalho, S.M., Carvalho, G.A., Carvalho, C.F., Bueno Filho, J.S.S., & Baptista, A.P.M. (2009). Toxicidade de acaricidas/inseticidas empregados na citricultura para a abelha africanizada Apis mellifera L., 1758 (Hymenoptera: Apidae). Arquivos do Instituto Biológico, 76:597-606. Retrived from: http://www.biologico.sp.gov.br/uploads/docs/arq/v76_4/carvalho.pdf

Ciarlo, T.J., Mullin, C.A., Frazier, J.L. & Schmehl, D.R. (2012). Learning impairment in honey bees caused by agricultural spray adjuvants. PLoS One, 7: e40848. doi: 10.1371/journal.pone.0040848

Coupe, R.H., Barlow, J.R. & Capel, P.D. (2012). Complexity of human and ecosystem interactions in an agricultural landscape. Environmental Development, 4: 88-104. doi: 10.1016/j.envdev.2012.09.009

Cox, R.L. & Wilson, W.T. (1984) Effects of permethrin on the behavior of individually tagged honey bees, Apis mellifera L. (Hymenoptera: Apidae). Environmental Entomology, 13:375-378.

Dahlgren, L., Johnson, R.M., Siegfried, B.D., & Ellis, M.D. (2012). Comparative toxicity of acaricides to honey bee (Hymenoptera: Apidae) workers and queens. Journal of Economic Entomology, 105: 1895-1902. doi: 10.1155/2014/179691

Della Lucia, T., Gandra, L.C. & Guedes, R.N. (2014). Managing leaf cutting ants: peculiarities, trends and challenges. Pest Management Science, 70: 14-23. doi: 10.1002/ps.3660

Ecobichon, D.J. (2001). Pesticide use in developing countries. Toxicology, 160: 27-33. doi: 10.1016/S0300-483X(00)00452-2

Fürst, M.A., McMahon, D.P., Osborne, J.L., Paxton, R.J. & Brown, M.J.F. (2014). Disease associations between honeybees and bumblebees as a threat to wild pollinators. Nature, 506:364-366. doi: 10.1038/nature12977

Gallai, N., Salles, J.M., Settele, J., & Vaissière, B.E. (2009). Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecological Economics, 68: 810-821. doi: 10.1016/j.ecolecon.2008.06.014

Giannini, T.C., Cordeiro, G.D., Freitas, B.M, Saraiva, A.M. & Imperatriz-Fonseca, V.L. (2015). The dependence of crops for pollinators and the economic value of pollination in Brazil. Journal of Economic Entomology, 108: 849-857. doi:10.1093/jee/tov093

Genersch, E. (2010). Honey bee pathology: current threats to honey bees and beekeeping. Applied Microbiology and Biotechnology, 87: 87-97. doi: 10.1007/s00253-010-2573-8

Goulson D. (2015) Neonicotinoids impact bumblebee colony fi tness in the fi eld; a reanalysis of the UK’s Food & Environment Research Agency 2012 experiment. PeerJ 3:e854. doi: 10.7717/peerj.854

Guedes, R.N.C., Smagghe, G., Stark, J.D. & Desneux, N. (2016) Pesticide induced stress in arthropod pests for optimized integrated pest management programs. Annual Review of Entomology, 61: 1–20. doi: 10.1146/annurevento-010715-023646

Ingram, E.M., Agustin, J., Ellis, M.D., Siegfried, B.D. (2015). Evaluating sub-lethal effects of orchard-applied pyrethroids using video-tracking software to quantify honey bee behaviors. Chemosphere, 135: 272-277. doi: 10.1016/j.chemosphere.2015.04.022

Imperatriz-Fonseca, V.L. & Nunes-Silva, P. (2010). As abelhas, os serviços ecossistêmicos e o Código Florestal Brasileiro/Bees, ecosystem services and the Brazilian Forest Code. Biota Neotropica, 10: 59. doi: 10.1590/S1676-06032010000400008

Johnson, R.M., Ellis, M.D., Mullin, C.A., Frazier, M. (2010). Pesticides and honey bee toxicity–USA. Apidologie, 4: 312- 331. doi: 10.1051/apido/2010018

Kerr, W.E., Carvalho, G.A. & Nascimento, V.A. (1996). Abelha uruçu: biologia, manejo e conservação. Paracatu: FundaçãoAcangaú. 143 p.

Kinasih, I., Nugraha, R.S., Putra, R.E., Permana, A.D., & Rosmiati, M. (2017). Toksisitas beberapa jenis fungisida komersial pada serangga penyerbuk, Trigona (Tetragonula) laeviceps Smith. Jurnal Entomologi Indonesia, 14: 29. doi: 10.5994/jei.14.1.29

Li-Byarlay, H., Rittschof, C.C., Massey, J. H., Pittendrigh, B. R. & Robinson, G. E. (2014). Socially responsive effects of brain oxidative metabolism on aggression. Proceedings of the National Academy of Sciences, 111:12533-12537. doi: 10.1073/pnas.1412306111

Leblois, A., Damette, O. & Wolfersberger, J. (2016). What has Driven Deforestation in Developing Countries Since the 2000s? Evidence from New Remote-Sensing Data. World Development, 92: 82-102. doi: 10.1016/j.worlddev.2016.11.012

Lonsdorf, E., Kremen, C., Ricketts, T., Winfree, R., Williams, N. & Greenleaf, S. (2009). Modelling pollination services across agricultural landscapes. Annals of Botany, 103: 1589-1600. doi: 10.1093/aob/mcp069

Luttik, R., Arnold, G., Boesten, J.J.T.I., Cresswell, J., Hart, A., Pistorius, J. & Thompson, H. (2012). Scientifi c Opinion on the science behind the development of a risk assessment of Plant Protection Products on bees (Apis mellifera, Bombus spp. and solitary bees). European Food Safety Authority

Journal, 10: 2668. doi: 10.2903/j.efsa.2012.2668

MAPA. Ministério da Agricultura, Pecuária e Abastecimento. (2018). Agrofi t: sistema de agrotóxicos fi tossanitários. http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons>. (accessed date: 20 July, 2018).

Mesnage, R., Bernay, B., & Séralini, G.E. (2013). Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity. Toxicology, 313:122-128. doi: 10.10

/j.tox.2012.09.006

Mesnage, R., Defarge, N., Spiroux de Vendômois, J. & Séralini, G.E. (2014). Major pesticides are more toxic to human cells than their declared active principles. BioMed research international, 2014:8. doi: 10.1155/2014/179691

Martinello, M., Baratto, C., Manzinello, C., Piva, E., Borin, A., Toson, M., Mutinelli, F. (2017). Spring mortality in honey bees in northeastern Italy: detection of pesticides and viruses in dead honey bees and other matrices. Journal of Apicultural Research, 56: 239-254. doi: 10.1080/00218839.2017.1304878

Michener, C.D. (2007). The Bees of the World. Baltimore: Johns Hopkins, 953p.

Morais, C.R., Travençolo, B.A.N., Carvalho, S.M., Beletti, M.E., Santos, V.S.V., Campos, C.F., de Campos Júnior, E.O., Pereira, B.B., Carvalho Naves, M.P., de Rezende, A.A.A. & Spanó, M.A., Vieira, C.U., Bonetti, A.M. (2018). Ecotoxicological effects of the insecticide fi pronil in Brazilian native stingless bees Melipona scutellaris (Apidae: Meliponini). Chemosphere, 206: 632-642. doi: 10.1016/j.

chemosphere.2018.04.153

Mullin, C.A. (2015). Effects of ‘inactive’ingredients on bees. Current Opinion in Insect Science, 10: 194-200. doi: 10.1016/j.cois.2015.05.006

Mullin, C.A., Fine, J.D., Reynolds, R.D. & Frazier, M.T. (2016). Toxicological risks of agrochemical spray adjuvants: organosilicone surfactants may not be safe. Frontiers in Public Health, 4. doi: 10.3389/fpubh.2016.00092

Putra, R.E., Permana, A.D. & Kinasih, I. (2014). Application of Asiatic honey bees (Apis cerana) and stingless bees (Trigona laeviceps) as pollinator agents of hot pepper (Capsicum annuum L.) at local Indonesia farm system. Psyche: A Journal of Entomology, 17: 86-91. doi: 10.1155/2014/687979

R Development Core Team R (2016). A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna (2016). http://www.R-project.org/. (accessed date: 8 february, 2017).

Raven, P.H., Evert, R.F. & Eichhorn, S.E. (2001). Biologia Vegetal. Rio de Janeiro: Guanabara Koogan, 906p.

Rondeau, G., Sánchez-Bayo, F., Tennekes, H.A., Decourtye, A., Ramírez-Romero, R., & Desneux, N. (2014). Delayed and time-cumulative toxicity of imidacloprid in bees, ants and termites. Scientifi c Reports, 4: 5566. doi: 10.1038/srep05566

Sandrock, C., Tanadini, M., Tanadini, L.G., Fauser-Misslin, A., Potts, S.G. & Neumann, P. (2014). Impact of chronic neonicotinoid exposure on honeybee colony performance and queen supersedure. PLOS one, 9: e103592. doi: 10.1371/journal.pone.0103592

Schreinemachers, P. & Tipraqsa, P. (2012) Agriculture pesticides and land use intensifi cation in high, middle and low income countries. Food Policy 37:616–626. doi: 10.1016/j.foodpol.2012.06.003

Silva, I.P., Oliveira, F.A.S., Pedroza, H.P., Gadelha, I.C.N., Melo, M.M. & Soto-Blanco, B. (2015). Pesticide exposure of honeybees (Apis mellifera) pollinating melon crops. Apidologie, 46:703-715. doi: 10.1007/s13592-015-0360-3

Silva, M. B., Nocelli, R.C.F., Soares, H.M., Malaspina, O. 2016. Efeitos do imidacloprido sobre o comportamento das abelhas Scaptotrigona postica Latreille, 1807 (Hymenoptera, Apidae). Revista Ciência, Tecnologia & Ambiente, 3: 21-28. Retrived from: http://www.revistacta.ufscar.br/index.php/revistacta/article/view/21

Syromyatnikov, M.Y., Kokina, A.V., Lopatin, A.V., Starkov, A.A., & Popov, V.N. (2017). Evaluation of the toxicity of fungicides to fl ight muscle mitochondria of bumblebee (Bombus terrestris L.). Pesticide Biochemistry and Physiology, 135: 41-46. doi: 10.1016/j.pestbp.2016.06.007

Whitehorn, P.R., O’connor, S., Wackers, F.L., & Goulson, D. (2012). Neonicotinoid pesticide reduces bumble bee colony growth and queen production. Science, 336: 351-2

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Published

2018-10-11

How to Cite

Leite, D. T., Sampaio, R. B., dos Santos, C. O., dos Santos, J. N., Chambó, E. D., de Carvalho, C. A. L., & Sodré, G. da S. (2018). Toxicity of Fenpyroximate, Difenoconazole and Mineral Oil on Apis mellifera L. Introduction. Sociobiology, 65(4), 737–743. https://doi.org/10.13102/sociobiology.v65i4.3416

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Section

Research Article - Bees

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