Comparative molecular cytogenetics in Melipona Illiger species (Hymenoptera, Apidae)

Vanderly Andrade-Souza, Olivia Maria Pereira Duarte, Cinthia Caroline Cardoso Martins, Igor Silva Santos, Márcio Gilberto Cardoso Costa, Marco Antonio Costa

Abstract


Cytogenetic studies in Melipona are scarce with only 24 species analyzed cytogenetically. Of these, six species had the rDNA sites physically mapped and characterized by Fluorescent in situ Hybridization (fish). The aim of this study was to perform karyotype analyzes on Melipona species from different regions of Brazil, with a greater sampling representative of the Amazonian fauna and using conventional, fluorochrome staining and FISH with heterologous rDNA probes. The predominant chromosome number was 2n = 18, however, the subspecies M. seminigra abunensis and M. s. pernigra showed 2n = 22 chromosomes. The karyotypes were symmetrical, however M. bicolor, M. quadrifasciata, M. flavolineata, M. fuscopilosa, M. nebulosa presented the first pair heteromorphic in length. CMA3+ blocks also exhibited heteromorphism of size and in almost all cases coincided with rDNA sites, except for M. crinita and M. nebulosa, which presented additional non-coincident CMA3+ blocks. The CMA/ rDNA sites were terminal and interstitial in species with high heterochromatic content, and pericentromeric in those species with low heterochromatic content. In addition to pointing out cytogenetic features of cytotaxonomic importance, the reorganization of the genome in Melipona is discussed.

Keywords


FISH; ribosomal genes; fluorochromes; heteromorphism; Amazonian bee fauna

Full Text:

PDF

References


Brito, R.M., Caixeiro, A.P.A., Pompolo, S.G. & Azevedo, G.G. (2003). Cytogenetic data of Partamona peckolti (Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/ CMA3 and DA/DAPI. Genetics and Molecular Biology, 26: 53-57. doi: 10.1590/S1415-47572003000100009

Cabral, J.S., Félix, L.P. & Guerra, M. (2006). Heterochromatin diversity and its colocalization with 5S and 45S rDNA sites in chromosomes of four Maxillaria species (Orchidaceae). Genetics and Molecular Biology, 29: 659-664. doi: 10.1590/S1415-47572006000400015

Cabrero, J. & Camacho, J.P.M. (2008). Location and expression of ribosomal RNA genes in grasshoppers: abundance of silent and cryptic loci. Chromosome Research, 16: 595-607. doi: 10.1007/s10577-008-1214-x

Camargo, J.M.F. & Pedro, S.R.M. (2013). Meliponini Lepeletier, 1836. In Moure, J. S., Urban, D. & Melo, G.A.R. (Orgs). Catalogue of Bees (Hymenoptera, Apoidea) in the Neotropical Region - online version. Available at http://www.moure.cria.org.br/catalogue. Accessed Apr/14/2018

Carabajal Paladino, L., Papeschi, A., Lanzavecchia, S., Cladera, J. & Bressa, M.J. (2013). Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent European. Journal of Entomology, 110: 401-409. doi: 10.14411/eje.2013.054

Fernandes, C.A. & Martins-Santos, I.C. (2006). Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil. Genetics and Molecular Biology, 29: 464-468. doi: 10.1590/S1415-47572006000300011

Fernandes-Salomão, T.M., Muro-Abad, J.I., Campos, L.A.O. & ARAÚJO, E.F. (2002). Mitochondrial and nuclear DNA characterization in the Melipona species (Hymenoptera, Meliponini) by RFLP analysis. Hereditas. 137, 229-233. doi: 10.1034/j.1601-5223.2002.01685.x

Francini, I.B., Gross, M.C., Nunes-Silva, C.G. & Carvalho-Zilse, G.A. (2011). Cytogenetic analysis of the Amazon stingless bee Melipona seminigra merrillae reveals different chromosome number for the genus. Scientia Agricola (Piracicaba, Braz.), 68: 592-593. doi: 10.1590/S0103-90162011000500012

Guerra, M. & Souza, M.J. (2002). Como Observar Cromossomos: Um Guia de Técnicas em Citogenética Vegetal Animal e Humana. FUNPEC Editora, Ribeirão Preto. 131 p.

Hanson, R.E., Islam-Faridi, M.N., Percival, E.A., Crane, C.F., Yuanfu, J.I., Mcknight, T.D., Stelly, D.M. & Price, H.J. (1996). Distribution of 5S and 18S–28S rDNA loci in a tetraploid cotton (Gossypium hirsutum L.) and its putative diploid ancestors. Chromosoma, 105: 55-61. doi: 10.1007/BF02510039

Hirai, H., Yamamoto, M., Taylor, R.W. & Imai, H.T. (1996). Genomic dispersion of 28S rDNA during karyotypic evolution in the ant genus Myrmecia (Formidae). Chromosoma, 105:190-196. doi: 10.1007/BF02529753

Imai, H.T., Taylor, R.W., Crosland, M.W. & Crozier, R.H. (1998). Modes of spontaneous chromosomal mutation and karyotype evolution in ants with reference to the minimum interaction hypothesis. Japanese Journal of Genetics, 63: 159-185. doi: 10.1266/jjg.63.159

Levan, A., Fredgam K. & Sandberg, A.A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52: 201-220. doi: 10.1111/j.1601-5223.1964.tb01953.x

Lopes, D.M., Pompolo, S.G., Campos, L.A.O. & Tavares, M.G. (2008). Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes staining. Genetics and Molecular Biology, 31: 49-52. 10.1590/S1415-47572008000100010

Lopes, D.M., Fernandes, A. Praça-Fontes, M.M. Werneck, H.A. & Resende, H.C. (2011). Cytogenetics of three Melipona species (Hymenoptera, Apidae, Meliponini). Sociobiology, 58: 185-194.

Loreto, V., Cabrero, J., López-León, M.D., Camacho, J.PM. & Sousa, M.J. (2008). Comparative analysis of rDNA location in five Neotropical gomphocerine grasshopper species. Genetica, v.132, p. 95-101, 2008. doi: 10.1007/s10709-007-9152-7

Mandrioli, M., Manicardi, G.C., Bizarro, D. & Bianchi, U. (1999). NOR heteromorphism within a parthenogenetic lineage of the aphid Megoura viciae. Chromosome Research, 7: 157-162. doi: 10.1023/A:1009215721904

Marthe, J.B., Pompolo, S.G., Campos, LAO, Fernandes-Salomão, T.M. & Tavares, M.G. (2010). Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes. Genetics and Molecular Biology, 33: 253-255. doi: 10.1590/S1415-47572010005000029

Martínez-Expósito, M.J., Méndez, J. & Pasantes, J.J. (1997). Analysis of NORs and NOR-associated heterochromatin in the mussel Mytilus galloprovincialis Lmk. Chromosome Research, 5: 268-273. doi: 10.1023/A:1018475804613

Michener, C.D. (2007). The bees of the world. 2nd ed. The John Hopkins University Press, Baltimore, Maryland. 953 p.

Moscone, E. A., Matzke, M.A & Matzke, A.J.M. (1996). The use of combined FISH/GISH in conjunction with DAPI counterstaining to identify chromosomes containing transgenes inserts in amphidiploid tobacco. Chromosoma, 105: 231-236. doi: 10.1007/BF02528771

Moure, J.S., Urban, D. & Melo, G.A.R. (Orgs). (2007) Catalogue of Bees (Hymenoptera, Apoidea) in the Neotropical Region. Online version. Available at http://www.moure.cria.org.br/catalogue. Accessed Jul/26/2018

Nguyen, P., Sahara, K., Yoshido, A. & Marec, F. (2010). Evolutionary dynamics of rDNA clusters on chromosomes of moths and butterflies (Lepidoptera). Genetica, 138: 343-354. doi: 10.1007/s10709-009-9424-5

Palomeque, T. & Lorite, P. (2008). Satellite DNA in insects: a review. Heredity, 100: 564-573. doi: 10.1038/hdy.2008.24

Pedro, S.R.M. (2014). The Stngless Bee Fauna In Brazil (Hymenoptera: Apidae). Sociobiology, 61: 348-354. doi: 10.13102/sociobiology.v61i4.348-354

Rafael, M.S., Tadei, W.P. & Pimentel, S.M.R. (2003). Location of Ribosomal Genes in the Chromosomes of Anopheles darlingi and Anopheles nuneztovari (Diptera, Culicidae) from the Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, 98: 629-635. doi: 10.1590/S0074-02762003000500008

Ramirez, S.R., Nieh, J.C., Quental, TB., Roubik, D.W., Imperatriz-Fonseca, V.L. & Pierce, N.E. (2010). A molecular phylogeny of the stingless bee genus Melipona (Hymenoptera: Apidae). Molecular Phylogenetics and Evolution, 56: 519-525. doi: 10.1016/j.ympev.2010.04.026

Ran, Y., Murray, B.G. & Hammett, K.R.W. (1999). Karyotype analysis of the genus Clivia by Giemsa and fluorochrome banding and in situ hybridization. Euphytica, 106: 139-147. doi: 10.1023/A:1003572705445

Rasmussen, C. & Cameron, S.A. (2010). Global stingless bee phylogeny supports ancient divergence, vicariance, and long distance dispersal. Biological Journal of the Linnean Society, 99: 206-232. doi: 10.1111/j.1095-8312.2009.01341.x

Rocha, M.P. & Pompolo S.G. (1998). Karyotypes and heterochromatin variation (C-bands) in Melipona species (Hymenoptera, Apidae, Meliponinae). Genetics and Molecular Biology, 21: 41-45. doi: 10.1590/S1415-47571998000100008

Rocha, M.P., Pompolo, S.G., Dergam, J.A., Fernandes, A. & Campos, L.A.O. (2002). DNA characterization and karyotypic evolution in the bee genus Melipona (Hymenoptera, Meliponini). Hereditas, 136: 19-27. doi: 10.1034/j.1601-5223.2002.1360104.x

Rocha, M.P., Cruz, M.P., Fernandes, A., Waldschmidt, A.M., Silva-Junior, J.C. & Pompolo, S. G. (2003). Longitudinal differentiation in Melipona mandacaia (Hymenoptera, Meliponini) chromosomes. Hereditas, 138: 133-137. doi: 10.1034/j.1601-5223.2003.01699.x

Rocha, M.P., Pompolo, S.G., Dergam, J.A., Fernandes, A & Campos, L.A. O. (2007). Melipona, Seis décadas de citogenética. (Melipona, six decades of cytogenetics). Bioscience Journal, 23: 111-117.

Roy, V., Monti-Dedieu, L., Chaminade, N., Siljak-Yakovlev, S., Aulard, S., Lemeunier, F. & Montchamp-Moreau, C. (2005). Evolution of the chromosomal location of rDNA genes in two Drosophila species subgroups: ananassae and melanogaster. Heredity, 94: 388-395. doi: 10.1038/sj.hdy.6800612

Salanitro, L.B., Massaccesi, A.C., Urbisaglia, S., Bressa, M.J. & Chirino, M.G. (2017). A karyotype comparison between two species of bordered plant bugs (Hemiptera, Heteroptera, Largidae) by conventional chromosome staining, C-banding and rDNA-FISH. Comparative Cytogenetics, 11: 239-248. doi: 10.3897/CompCytogen.v11i2.11683

Silveira, F.A., Melo, G.A.R. & Almeida, E.A.B. (2002). Abelhas do Brasil: Sistemática e Identificação. 1a. Ed. Belo Horizonte: edição dos Autores, 254 p.

Śliwińska-Jewsiewicka, A., Kuciński, M., Kirtiklis, L., Dobosz, S., Ocalewicz, K. & Jankun, M. (2015). Chromosomal characteristics and distribution of rDNA sequences in the brook trout Salvelinus fontinalis (Mitchill, 1814). Genetica. 143: 425-432. doi: 10.1007/s10709-015-9841-6

Sochorová, J., Garcia, S., Gálvez, F., Symonová, R. & Aleš Kovařík. (2018). Evolutionary trends in animal ribosomal DNA loci: introduction to a new online database. Chromosoma, 127: 141-150. doi: 10.1007/s00412-017-0651-8

Tavares, M.G., Lopes, D.M. & Campos, L.A.O. (2017). An overview of cytogenetics of the tribe Meliponini (Hymenoptera: Apidae). Genetica, 145:241-258. doi: 10.1007/s10709-017-9961-2.

Vershinina, A., Anokhin, B. & Lukhtanov, V. (2015). Ribosomal DNA clusters and telomeric (TTAGG)n repeats in blue butterflies (Lepidoptera, Lycaenidae) with low and high chromosome numbers. Comparative Cytogenetics, 9: 161-171. doi: 10.3897/CompCytogen.v9i2.4715

Vitturi, R., Colomba, M.S., Barbieri, R. & Zunino, M. (1999). Ribosomal DNA location in the scarab beetle Thorectes intermedius (Costa) (Coleoptera: Geotrupidae) using banding and fluorescent in-situ hybridization. Chromosome Research, 7: 255-260. doi: 10.1023/A:1009270613012.

Wanzenböck, E.M., Schöfer, C., Schweizer, D. & Bachmair, A. (1997). Ribosomal transcription units integrated via T-DNA transformation associate with the nucleolus and do not require upstream repeat sequences for activity in Arabidopsis thaliana. Plant Journal, 11: 1007-1016. doi: 10.1046/j.1365-313X.1997.11051007.x




DOI: http://dx.doi.org/10.13102/sociobiology.v65i4.3480

Refbacks

  • There are currently no refbacks.


JCR Impact Factor 2016: 0.699