Period and Time of Harvest Affects the Apitoxin Production in Apis mellifera Lineu (Hymenoptera, Apidae) Bees and Expression of Defensin Stress Related Gene
Keywords:honeybees, collection, venom, welfare
The aims of this study were to determine the period effects (morning and afternoon) and harvest time (30 and 60 minutes) in the apitoxin production as well as the management effects in the expression of a stress related gene. Five Apis mellifera L. beehives were used. The harvest of apitoxin and honeybees occurred three times a week (morning and afternoon at 09h00 and 14h00), according to the following treatments (period and apitoxin harvest time): T1: morning/30 minutes, T2: morning/60 minutes; T3: afternoon/30 minutes; T4: afternoon/60 minutes. The apitoxin was collected by electric collectors. The stress level was monitored by defensin gene expression, using actin gene as control. The results were evaluated by ANOVA followed by Tukey-Kramer test (p ≤ 0.05). Its can be concluded that the better period and time to apitoxin harvest is in the morning for 60 minutes, associated to minor stress for honeybees.
Abreu, R. M., Moraes, R. L. M. S. & Mathias, M. I. C. (2010) Biochemical and cytochemical studies of the enzymatic activity of the venom glands of workers of honey bee Apis mellifera L. (Hymenoptera, Apidae). Micron. 41: 172-175. doi: 10.1016/j.micron.2009.09.003
Brandeburgo, M. A. M. & Gonçalves, L. S. (1990) Environmental influence on the aggressive (defense) behaviour and colony development of africanized bees (Apis mellifera). Cienc. e Cult. 42: 759-771. http://www.cabdirect.org/abstracts/19920510324.html (Accessed date: 28 June, 2014)
Lomele, R. L., Evangelista, A., Ito M. M., Ito, E. H., Gomes, S. M. A. & Orsi R. O. (2010) Natural products on the defensive behavior of Apis mellifera L. Acta. Scientarium Anim. Sci. 32: 285-291. doi: 10.4025/actascianimsci.v32i3.8486
Funari, S. R. C., Rocha, H. C., Sforcin, J. M. & Orsi, R. O. (2004) Influence of smoke and lemon grass (Cymbopogon citratus) on the defensive behavior of africanized bees and their hybrid European (Apis mellifera L). Bol. Indústria Anim. 61: 121-125. http://revistas.bvs-vet.org.br/bia/article/view/8704. (Accessed date: 28 June, 2014)
Leite, G. L. D. & Rocha, S. L. (2005) - Apitoxin. UniMont. Cient.-Montes Claros 7: 115-125. http://www.ruc.unimontes.br/index.php/unicientifica/article/viewArticle/145. (Accessed date: 28 June, 2014)
Malerbo-Souza, D. T. & Silva, F. A. S. (2011) Comportamento forrageiro da abelha africanizada Apis mellifera L. no decorrer do ano. Acta Scientarium Anim. Sci. 33: 183-190.
Pfaffl, M. (2001) A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Res. 45: 29. doi: 10.4025/actascianimsci.v33i2.9252
Scharlaken, B., Graaf, D. C., Goossens, K., Peelman, L. J. & Jacobs F. J. (2008) Differential gene expression in the honeybee head after a bacterial challenge. Dev. & Comp. Immunol. 32: 883-889. doi: 10.1016/j.dci.2008.01.010
Stort, A. C. (1975) Genetic study of the aggressiveness of two subspecies of Apis mellifera in Brazil. Behav. Genet. 3: 269-274. http://link.springer.com/article/10.1007/BF01066178. (Accessed date: 28 June, 2014)
Ursic-Bedoya, R. J. & Lowenberger, C.A. (2007) Rhodnius prolixus: Identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization. Dev. & Comp. Immunol. 31: 109–120. doi: 10.1016/j.dci.2006.05.008
Zar, J. H. (1996) Biostatistical analisys. 4th ed. New Jersey: Prentice Hall 663 p.
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