Identification of the Tandem Running Pheromone in Diacamma sp. from Japan (Hymenoptera, Formicidae)


  • Naoki Fujiwara Tsujii National Institute of Agrobiological Sciences
  • Kotone Tokunaga Chemical Ecology laboratory, Kyoto Institute of Technology
  • Toshiharu Akino Chemical Ecology laboratory, Kyoto Institute of Technology
  • Kazuki Tsuji Faculty of Agriculture, University of the Ryukyus
  • Ryohei Yamaoka Chemical Ecology laboratory, Kyoto Institute of Technology



tandem running, nest emigration, chemical communication, Formicidae, ( )-8-heptadecene.


The Japanese queenless ponerine ant Diacamma sp. from Japan employs tandem running during nest relocation, in which a leader ant guides nestmate followers one at a time. We replicated this process by presenting one entire abdominal part of a leader, except for the petiole to followers. When the abdominal part had been rinsed with n-hexane, however, it attracted significantlyfewer followers. This suggests that chemicals on the leader’s abdominal part evoke tandem running. Dissection of abdominal major exocrine glands revealed that the Dufour’s gland was the source of this chemical signal. The chemicals were eluted in the hydrocarbon fraction by silica-gel column chromatography, and the quantitatively major component was estimated as heptadecene (C17:1) through gas chromatograph-mass spectrometer (GCMS) analysis. The position of the double bond was estimated to be between the 8th and 9th carbons through analysis of the epoxidized compound. Only (Z)-isomers of 8-heptadecene evoked tandem running in the followers. We identified the tandem running pheromone of this ant species to be (Z)-8- heptadecene. (163)


Bestmann, H.-J., E. Janssen, F. Kern, B. Liepold, B. Hölldobler & T. Boveri 1995. All-transgeranylgeranyl acetate and geranylgeraniol, recruitment pheromone components in the Dufour gland of poneromorph ant Ectatomma ruidum. Naturwissenschaften 82: 334-336.

Blatrix, R., C. Schulz, P. Jaisson, W. Francke & A. Hefetz 2002. Trail pheromone of the ponerine ant Gnamptogenys striatula: 4-methylgeranylesters from Dufour’s gland. Journal of Chemical Ecology 28: 2557-2567.

Fukumoto, Y. & T. Abe 1983. Social organization of colony movement in the tropical ponerine ant, Diacamma rugosum (Le Guillou). Journal of Ethology 1: 101-108.

Hölldobler, B. & E.O. Wilson 1978. The multiple recruitment systems of the African weaver ant Oecophyla longinoda (Latreille) (Hymenoptera: Formicidae). Annals of the Entomological Society of America 76: 235-238.

Hölldobler, B. & E.O. Wilson 1990. The Ants. Harvard University Press, Cambridge, MA.

Hölldobler, B. & J.F.A. Traniello 1980a. Tandem running pheromone in ponerine ants. Naturwissenschaften 67: 360.

Hölldobler, B. & J.F.A. Traniello 1980b. The pygidial gland and chemical recruitment communication in Pachycondyla (=Termitopone) laevigata. Journal of Chemical Ecology 6: 883-893.

Howard, R.W. 1993. Cuticular hydrocarbons and chemical communication. In: Stanley- Samuelson, D.W. & D.R. Nelson (eds.) Insect Lipids: Chemistry, Biochemistry and Biology. University of Nebraska Press, Lincoln, NE: 179–226.

Maschwitz, U., K. Jessen & S. Knecht 1986. Tandem recruitment and trail laying in the ponerine ant Diacamma rugosum: signal analysis. Ethology 71: 20-41.

Möglich, M., U. Maschwitz & B. Hölldobler 1974. Tandem calling: A new signal in ant communication. Science 186: 1046-1047.

Morgan, E.D. 2009. Trail pheromone of ants. Physiological Entomology 34: 1-17.

Swarn, D. 1953. Epoxidation and hydroxylation of ethylenic compounds with organic peracids. Organic reaction 7: 378-380.

Traniello, J.F.A. & B. Hölldobler 1984. Chemical communication during tandem running in Pachycondyla obscuricornis (Hymenoptera: Formicidae). Journal of Chemical Ecology 10: 783-794.

Vander Meer, R.K., F.D. Williams & C.S. Lofgren 1981. Hydrocarbon components of the trail recruitment pheromone of the red imported fire ant, Solenopsis invicta. Tetrahedron Letters 22: 1651-1654.

Vander Meer, R.K., F.M Alvarez & C.S. Lofgren 1988. Isolation of the trail recruitment pheromone of Solenopsis invicta. Journal of Chemical Ecology 14: 825-838.

Vander Meer, R.K., C.S. Lofgren & F.M Alvarez 1990. The orientation inducer pheromone of the fire ant Solenopsis invicta. Physiological Entomology 15: 483-488.

Vander Meer, R.K. & L. Morel 1998. Pheromone directed behavior in ants. In: Vander Meer, R.K., M.D. Breed, M.L. Winston & K.E. Espelie (eds.) Pheromone Communication in Social Insects: Ants, Wasps, Bees, and Termites. Westview Press, Boulder, Colorado: 176-182.

Wilson, E.O. 1971. The Insect Societies. Belknap Press of Harvard Univ. Press, Cambridge.




How to Cite

Tsujii, N. F., Tokunaga, K., Akino, T., Tsuji, K., & Yamaoka, R. (2014). Identification of the Tandem Running Pheromone in Diacamma sp. from Japan (Hymenoptera, Formicidae). Sociobiology, 59(4), 1281–1295.