Genetic Divergence of Turkish Apis mellifera Subspecies Based on Sequencing of ND5 Mitochondrial Segment

Authors

  • Fulya Ozdil Selçuk University
  • Fatma İIlhan Selçuk University, Faculty of Agriculture, Department of Animal Science

DOI:

https://doi.org/10.13102/sociobiology.v59i1.679

Keywords:

Apis mellifera L., DNA sequence diversity, ND5, Turkey

Abstract

Mitochondrial DNA sequence variation can be used to infer honey bee evolutionary relationships. In this study, DNA sequence diversity in the ND5 region of the mitochondrial genome was investigated in 93 samples of Apis mellifera from 15 different populations in Turkey. Five novel haplotypes were revealed for the ND5 gene segment of Turkish honeybees. The number of variable sites found was 6 for this region while 2 were parsimony informative sites. The average pairwise genetic distances were 0.3% for ND5 gene. In this study, the NJ tree of ND5 gene segment were constructed with the published sequences of Apis mellifera haplotypes. This study expands the knowledge about the mitochondrial ND5 region in Apis mellifera and it is also the first comprehensive sequencing analysis of ND5 region in Turkish honeybees.

Downloads

Download data is not yet available.

References

Arias, M.C. & W.S. Sheppard 1996. Molecular phylogenetics of honey bee subspecies (Apis mellifera L.) inferred from mitochondrial DNA sequence. Molecular Phylogenetics and Evolution 5: 557–566.

Bouga, M., P.C. Harizanis, G. Kilias & S. Alahiotis 2005. Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR-RFLP analysis of three mtDNA segments. Apidologie 36: 335–344.

Bouga, M., C. Alaux, M. Bienkowska, R. Buchler, N.L. Carreck, E. Cauia, R. Chlebo, B. Dahle, R. Dall’olio, P. De La Rua, A. Gregorc, E. Ivanova, A. Kence, M. Kence, N. Kezic, H. Kiprijanovska, P. Kozmus, P. Kryger, Y. Le Conte, M. Lodesani, A. M. Murilhas, A. Siceanu, G. Soland, A. Uzunov & J. Wilde 2011. A review of methods for discrimination of honey bee populations as applied to European beekeeping. Journal of Apicultural Research 50(1): 51-84.

Crozier, R.H. & Y.C. Crozier 1993. The mitochondrial genome of the honeybee Apis mellifera: complete sequence and genome organization. Genetics 133: 97–117.

De la Rua, P., J. Galian, J. Serrano & R.F.A. Moritz 2001. Genetic structure and distinctness of Apis mellifera L. populations from the Canary Islands. Molecular Ecology 10: 1733–1742.

De la Rua, P., R. Hernandez-Garcia, B.V. Pedersen, J. Galian & J. Serrano 2004. Molecular diversity of honeybee Apis mellifera iberica L. (Hymenoptera: Apidae) from western Andalusia. Archiv Zootec 53: 195–203.

Franck, P., L. Garnery, M. Solignac & J.-M. Cornuet 2000. Molecular confirmation of a fourth lineage in honeybees from the near east. Apidologie 31: 167–180.

Franck, P., L. Garnery, A. Loiseau, B.P. Oldroyd, H.R. Hepburn, M. Solignac & J.-M. Cornuet 2001. Genetic diversity of the honeybee in Africa: microsatellite and mitochondrial data. Heredity 86: 420–430.

Garnery, L., J.-M. Cornuet & M. Solignac 1992. Evolutionary history of the honey bee Apis mellifera inferred from mitochondrial DNA analysis. Mol. Ecol. 1: 145–154.

Garnery, L., M. Solignac, G. Celebrano & J.-M. Cornuet 1993. A simple test using restricted PCR amplified mitochondrial DNA to study the genetic structure of Apis mellifera. Experientia 49: 1016–1021.

Hall, H.G. 1990. Parental analysis of introgressive hybridization between African and European honeybees using nuclear DNA RFLPs. Genetics 125: 611–621.

Hall, H.G. & D.R. Smith 1991. Distinguishing African and European honeybee matrilines using amplified mitochondrial DNA. Proc. Natl Acad. Sci. USA 88: 4548–4552.

Kandemir, I., M. Kence, W.S. Sheppard, A. Kence 2006. Mitochondrial DNA variation in honey bee (Apis mellifera L.) populations from Turkey. Journal of Apicultural Research and Bee World 45 (1): 33–38.

Kauhausen-Keller, D, F. Ruttner & R. Keller 1997. Morphometric studies on the microtaxonomy of the species Apis mellifera L. Apidologie 28: 295–307.

Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111–120.

Kozmus, P., J. Stevanović, Z. Stanimirović, V. Stojić, Z. Kulišić & V. Meglič 2007. Analysis of mitochondrial DNA in honeybees (Apis mellifera) from Serbia. Acta Vet. 57: 465–476.

Magnus, R. & A.L. Szalanski 2010. Genetic Evidence for Honey Bees (Apis mellifera L.) of Middle Eastern Lineage in the United States. Sociobiology 55: 285–296.

Martimianakis, S., E. Klossa-Kilia, M. Bouga & G. Kilias 2011. Phylogenetic relationships of Greek Apis mellifera subspecies based on sequencing of mtDNA segments (COI and ND5). Journal of Apicultural Research 50 (1): 42–50.

Muňoz, I., R. Dall’Olio, M. Lodesani & P. De la Rua 2009. Population genetic structure of coastal Croatian honeybees (Apis mellifera carnica). Apidologie 40: 617–626.

Nedić, N., L. Stanisavljević, M. Mladenović, J. Stanisavljević 2009. Molecular characterization of the honeybee Apis mellifera carnica in Serbia. Arch. Biol. Sci., Belgrade 61 (4): 587–598.

Ozdil ,F., M.A. Yıldız & H.G. Hall 2009a. Molecular Characterization of Turkish Honey Bee Populations (Apis mellifera L.) Inferred from Mitochondrial DNA RFLP and Sequence Results. Apidologie 40 (5): 570–576.

Ozdil, F., B. Fakhri, H. Meydan, M.A. Yıldız & H.G. Hall 2009b. Mitochondrial DNA variation in the COI-COII intergenic region among Turkish and Iranian Honey Bees (Apis mellifera L.). Biochemical Genetics 47: 717–721.

Ozdil,F., H. Meydan, M.A. Yıldız & H.G. Hall 2011. Genetic Diversity of Turkish Honey Bee Populations Based on RFLPs at a Nuclear DNA Locus. Sociobiology 58(3): 719–731.

Palmer, M.R., D.R. Smith & O. Kaftanoğlu 2000. Turkish honeybees: Genetic variation and evidence for a fourth lineage of Apis mellifera mtDNA. J. Hered. 91: 42–46.

Ruttner, F. 1988. Biogeography and Taxonomy of Honeybees.Springer-Verlag, Berlin. 284 pp.

Smith, D.R., A. Slaymaker, M. Palmer & O. Kaftanoğlu 1997. Turkish honeybees belong to the east Mediterranean mitochondrial lineage. Apidologie 28: 269–274.

Sušnik, S., P. Kozmus, J. Poklukar & V. Meglić 2004. Molecular characterization of indigenous Apis mellifera carnica in Slovenia. Apidologie 35: 623–636.

Szalanski,A.L. & R.M. Magnus 2010. Mitochondrial DNA characterization of Africanized honey bee (Apis mellifera L.) populations from the USA. Journal of Apicultural Research and Bee World 49(2): 177–185.

Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei & S. Kumar 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28(10): 2731–2739.

Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin & D.G. Higgins 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25: 4876–4882.

Downloads

Published

2014-10-22

How to Cite

Ozdil, F., & İIlhan, F. (2014). Genetic Divergence of Turkish Apis mellifera Subspecies Based on Sequencing of ND5 Mitochondrial Segment. Sociobiology, 59(1), 225–234. https://doi.org/10.13102/sociobiology.v59i1.679

Issue

Vol. 59 No. 1 (2012)

Section

Articles

License

Sociobiology is a diamond open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.

Authors who publish with this journal agree to the following terms:

 

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

 

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

 

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).