Wasmannia Forel (Hymenoptera: Formicidae: Myrmicinae) in Argentina: Systematics and Distribution


  • Fabiana del Carmen Cuezzo Instituto Superior de Entomologia (INSUE) Facultad de Ciencias Naturales e IML UEL (CONICET) Miguel Lillo 205 San Miguel de Tucumán, ARGENTINA
  • Luis Calcaterra Fundación para el Estudio de Especies Invasivas-CONICET, Bolívar 1559 (B1686EFA) Hurlingham, Buenos Aires, Argentina
  • Lucila Chifflet 3Universidad de Buenos Aires-CONICET, Facultad de Ciencias Exactas y Naturales, Grupo de Investigación en Filogenias Moleculares y Filogeografía. Intendente Güiraldes 2160 (C1428EGA), Ciudad Universitaria, Ciudad de Buenos Aires, Argentina
  • Peter Follet USDA-ARS, Pacific Basin Agricultural Research Center, PO Box 4459, Hilo, Hawaii 96720




Wasmannia longiseta n. sp., little fire ant, biogeography, taxonomy, phylogeny


The ant genus Wasmannia is endemic to the Neotropics, with 10 species occurring within the presumptive native range for the genus from Mexico to Argentina. Only the little fire ant, Wasmannia auropunctata is widely distributed being present from central-eastern Argentina to Bermuda, and has become infamous due to its recent worldwide expansion and status as an invasive pest. The objective of this work was to study the systematics and distribution of Wasmannia in its southern limit of distribution in Argentina. Out of the three species reported so far for Argentina, only W. auropunctata was found to be widely distributed, but abundant only in disturbed habitats mostly in the Northeast. Herein, the distribution of Wasmannia auropunctata is extended and its queen and male castes are redescribed, along with descriptions of gynandromorphs (specimens with left side of the head similar to a male and right side similar to a queen). Wasmannia sulcaticeps and W. williamsoni are much less common and widespread. W. sulcaticepsis mostly found in mountain forests in northwestern Argentina, whereas W. williamsoni is only found in shrublands and grasslands in central eastern Argentina, and most frequently in mountain grasslands. Both species overlap with W. auropunctata, which is more common in the lowlands, between approximately 400 and 1000 m elevation. The queen of W. williamsoni is described and queen and male of W. sulcaticeps are redescribed.  A new species, Wasmannia longiseta n. sp. Cuezzo and Calcaterra, recently found in northeastern Argentina is described based on worker morphology. Wasmannia rochai is recorded for the first time in Misiones, extending its distribution range from São Paulo (Brazil) to Misiones in northeastern Argentina. A key to the worker caste is provided. A cladistic analysis based on discrete and continuous morphological characters is presented as a first attempt to clarify the phylogenetic relationships between the known species of Wasmannia.


Berndt, K. P. & Kremer, G. (1982). Heat Shock-Induced Gynandromorphism in the Pharaoh's Ant Monomorium pharaonis (L.).Experientia 38:798–799.

Bolton, B. (2003). Synopsis and Classification of Formicidae. Mem. Am. Entomol. Inst.71: 1-370.

Cabrera, A.L. & A. Willink, A. (1980). Biogeografía de América Latina. Segunda edición. Monografía 13, serie biología. Programa Regional de Desarrollo Científico y Tecnológico, Organización de los Estados Americanos. 122 pp. + 1 map.

Calcaterra, L.A., Coulin, C., Briano, J.A. & Follett, P.A. (2012). Acute Exposure to Low Dose Radiation Disrupts Reproduction and Reduces Longevity in Wasmannia auropunctata (Hymenoptera: Formicidae) Queens. J. Econ. Entomol. 105: 817-822.

Cranston, P.S. & Humphries, C.J. (1988). Cladistics and Computers: a Chironomid Conundrum?. Cladistics 4: 72-92. http://dx.doi.org/10.1111/j.1096-0031.1988.tb00469.x

Cuezzo, F. (1998). Formicidae. In Biodiversidad de Artrópodos de la Argentina. Morrone, J.J. & S. Coscarón Eds. La Plata. Argentina, 1998: pp. 452-462.

De Fina, A.L. (1992). Aptitud Agroclimática de la República Argentina. Acad. Nac. Agron. y Vet., 402 pp.

De Souza, A.L.B., Delabie, J.H.C. & Fowler, H.G. (1998). Wasmannia spp. (Hym. Formicidae) and Insect Damages to Cocoa in Brazilian Farms. J. App. Entomol. 122: 339-341.

Emery, C. (1894). Studi Sulle Formiche della Fauna Neotropica. VI-XVI. Bull. Soc.Entomol. Italiana, 26: 137-241.

Fernández, F. (2003). The Myrmicine Ant Genera Ochetomyrmex Mayr and Tranopelta Mayr (Hymenoptera: Formicidae). Sociobiology 41: 633-661.

Fernández, F. (2007). The Myrmicine Ant Genus Allomerus Mayr (Hymenoptera: Formicidae). Caldasia 29: 159-175.

Fournier, D., Estoup, A., Orivel, J., Foucaud, J., Jourdan, H., Breton, J., & Keller, L. (2005). Clonal Reproduction by Males and Females in the Little Fire Ant. Nature 435: 1230-1234. http://dx.doi.org/10.1038/nature03705.

Foucaud, J., Fournier, D., Orivel, J., Delabie, J., Loiseau, A., Le Breton, J., Kergoat, G. & Estoup, A. (2007). Sex and Clonality in the Little Fire Ant. Molec. Biol. & Evol. 24: 2465–2473. http://dx.doi.org/10.1093/molbev/msm180.

Foucaud, J., Orivel, J., Fournier, D., Delabie, J.H.C., Loiseau, A., Le Breton, J., Cerdan, P., & Estoup, A. (2009). Reproductive System, Social Organization, Human Disturbance, and Ecological Dominance in Native Populations of the Little Fire Ant, Wasmannia auropunctata. Mol. Ecol. 18: 5059-5073. http://dx.doi.org/10.1111/j.1365-294X.2009.04440.x.

Foucaud, J., Orivel, J., Loiseau, A., Delabie, J.H.C, .Jourdan, H., Konghouleux, D., Vonshak, M., Tindo, M., Mercier, J.-L., Fresneau, D., Mikissa, J.-B., McGlynn, T., Mikheyev, A.S.,Oettler, J. & Estoup, A. (2010). Worldwide Invasion by the Little Fire Ant: Routes of Introduction and Eco-Evolutionary Pathways. Evol. Appl. 3:363-374. http://dx.doi.org/10.1111/j.1752-4571.2010.00119.x.

Forel, A. (1893). Formicides de l’Antille St. Vincent, Récoltées par Mons. H. H. Smith. Trans. Entomol. Soc. of London, 1893: 333-418.

Forel, A. (1908). Ameisen aus São Paulo (Brasilien), Paraguay etc. gesammelt von Prof. Herm. v.

Ihering, Dr. Lutz, Dr. Fiebrig, etc. Verh. der Kaiserlich-Königlichen Zool.-Bot. Ges. in Wien 58: 340-418.

Forel, A. (1912). Formicides Néotropiques. Part IV. 3me sous-famille Myrmicinae Lep. (suite). Mém. . Soc. Entomol. . Belg. 20: 1-32.

Fuentes, M.B., Cuezzo, F. & Di Iorio, O. (1988). Ant Species of the Natural Reserve of Otamendi, Buenos Aires, Argentina (Hymenoptera: Formicidae). Giornale It. Entomol. 9: 97-98.

Goloboff, P.A., Farris J.S., Källersjö, M., Oxelmann, B., Ramirez, M., and Szumik, C. (2003). Improvements to Resampling Measures of Group Support. Cladistics 19: 324-332. http://dx.doi.org/10.1111/j.1096-0031.2003.tb00376.x.

Goloboff, P.A., Mattoni C.I., Quinteros, A.S. (2006). Continuous Characters Analyzed as Such. Cladistics 22: 589-601. http://dx.doi.org/10.1111/j.1096-0031.2006.00122.x.

Goloboff, P.A., Farris, J.S., Nixon, K.C. (2008). TNT (Tree analysis using new technology) (BETA) ver. 1.1. Published by the authors, Tucumán, Argentina.

Goncalves, C.R. (1961). O Genero Acromyrmex no Brasil (Hym. Formicidae). Studia Entomol. (N. S.) 4: 113-180.

Jones, S.R., & Philips, S.A. Jr. (1985). Gynandromorphism in the Ant Pheidole dentate Mayr (Hymenoptera: Formicidae). Proc. Entomol. Soc. Wash. 87: 583–586.

Kempf, W.W. (1961). A Survey of the Ants of the Soil Fauna in Surinam (Hymenoptera: Formicidae). Stud.Entomol. 4: 481-524.

Kempf, W.W. (1965). A Revision of the Neotropical Fungus-Gowing Ants of the Genus Cyphomyrmex Mayr. Part II. Group of rimosus (Spinola) (Hym. Formicidae). Studia Entomol. 8: 161-200.

Kempf, W.W. (1967). Three New South American Ants (Hym. Formicidae). Studia Entomol. 10: 353-360.

Kusnezov, N. (1952). El género Wasmannia en la Argentina (Hymenoptera, Formicidae). Acta Zool. Lilloana, 10: 173-182.

Longino, J.T. & Fernández, F. (2007). Taxonomic Review of the Genus Wasmannia. In: Snelling, R.R., B.L. Fisher, and P.S. Ward (eds). Advances in Ant Systematics (Hymenoptera: Formicidae): homage to E. O. Wilson – 50 years of contributions. Mem. Am. Entomol. Inst. 80: 271-289.

Mikheyev, A. & Mueller, U. (2007). Genetic Relationships Between Native and Introduced Populations of the Little Fire Ant Wasmannia auropunctata. Diver. & Distrib. 13: 573-579. http://dx.doi.org/10.1111/j.1472-4642.2007.00370.x.

Orivel, J., Grangier J., & Foucaud J. (2009). Ecologically Heterogeneous Populations of the Invasive Ant Wasmannia auropunctata Within its Native and Introduced Ranges. Ecol. Entomol. 34:504-512.

Pearcy, M., Goodisman, M.A.D. & Keller, L. (2011). Sib Mating without Inbreeding in the Longhorn Crazy Ant. Proc. R. Soc. B, 278: 2677–2681. http://dx.doi.org/10.1098/rspb.2010.2562

Pimentel, R A. & Riggins, R. (1987). The Nature of Cladistic Data. Cladistics 3: 201-209. http://dx.doi.org/10.1111/j.1096-0031.1987.tb00508.x.

Rae, T.C. (1998). The Logical Basis for the Use of Continuous Characters in Phylogenetic Systematics. Cladistics 14: 221-228. http://dx.doi.org/10.1111/j.1096-0031.1998.tb00335.x.

Rey, O., Estoup, A., Vonshak, M., et al. (2012). Where do adaptive shifts occur during invasion? A multidisciplinary approach to unravelling cold adaptation in a tropical ant species invading the Mediterranean area. Ecol. Letters 15:1266–1275. doi: 10.1111/j.1461-0248.2012.01849.x.

Rigaud, T. & Juchault, P. (1993). Conflict Between Feminizing Sex Ratio Distorters and an Autosomal Masculinizing Gene in the Terrestrial Isopod Armadillidium vulgare Latr. Genetics 133: 247-252.

Roger, J. (1863). Die neu aufgeführten Gattungen und Arten meines Formiciden-Verzeichnissesnebst Ergänzung einiger früher gegebenen Beschreibungen. Berl. Entomol. Zeits. 7: 131-214.

Santschi, F. 1929. Nouvelles fourmis de la République Argentine et du Brésil. An. Soc. Cient. Arg. 107: 273-316.

Santschi, F. (1929). Nouvelles fourmis de la République Argentine et du Brésil. An. Soc. Cient. Arg.107: 273-316.

Schultz, T. R. & Meier, R. (1995). A Phylogenetic Analysis of the Fungus-Growing Ants (Hymenoptera: Formicidae: Attini) Based on Morphological Characters of the Larvae. Syst. Entomol. 20: 337-370. http://dx.doi.org/10.1111/j.1365-3113.1995.tb00100.x.

Sellés-Martínez, J. (2001). The Geology of Ventania (Buenos Aires Province, Argentina). J. Iberian Geol., 27: 43-69.

Serna, F. & Mackay, W. (2010). A Descriptive Morphology of the Ant Genus Procryptocerus (Hymenoptera: Formicidae). J. Insect Sc. 10 (111):1-36. doi: 10.1673/031.010.11101.

Serna, F., Bolton, B. & Mackay, W. (2011). On the Morphology of Procryptocerus. Some Comments and corrigenda. Zootaxa 2923: 67-68.

Snelling, R R. & Longino, J.T. (1992). Revisionary Notes of the Fungus Growing Ants of the Genus Cyphomyrmex rimosus Group (Hymenoptera: Formicidae: Attini). In Quintero, D. & A. Aiello (Eds.) Insects of Panamá and Mesoamerica: selected studies. Oxford University Press, Oxford. 692 pp.

Souza, A.L.B., Tavares, M.G., Serrao, J.E., & Delabie, J.H.C. (2009). Genetic Variability of Native Populations of Wasmannia Forel (Hymenoptera: Formicidae) and their Biogeographical Implications. Neotropical Entomology 38(3):376-383.

Stevens, P.F. (1991). Character States, Morphological Variation, and Phylogenetic Analysis: a Review. Syst. Bot. 16: 553-583. http://dx.doi.org/10.2307/2419343.

Ulloa Chacón, D. & Cherix, D. (1990). The Llittle Fire Ant Wasmannia auropunctata (Roger) (Hymenoptera: Formicidae). Pp. 281-289. In: Vander Meer, R. K., Jaffe, K. & Cedeno, A.(eds). Applied Myrmecology: a World Perspective: 741 pp. Westview Press, Boulder,Colorado, USA.

Vittar, F. & Cuezzo, F. (2008). Hormigas (Hymenoptera: Formicidae) de la Provincia de Santa Fe, Argentina. Rev. Soc. Entomol. Argent. 67: 175-178.

Ward, P.S., Brady, S.G., Fisher, B.L. & Schultz, T.R. (2014). The evolution of Myrmicine ants: phylogeny and biogeography of a hyperdiverse ant clade (Hymenoptera: Formicidae). Systematic Entomology. doi: 10.1111/syen.12090.

Wheeler, G.C. & Wheeler, J. (1976).Ant larvae: Review and synthesis. Entomological Society of Washington,108 pp.

Wheeler, G.C. & Wheeler, J. (1991). The Larva of Blepharidatta (Hymenoptera: Formicidae). Journal of the New York Entomological Society 99: 132-137.

Wheeler, W.M. (1915). Two New Genera of Myrmicinae Ants from Brazil. Bull. Mus. Comp. Zool., Harvard LIX 7: 482-491.

Wcislo, W.T., González, V.H. & Arneson, L.( 2004). A Review of Deviant Phenotypes in Bees in Relation to Brood Parasitism, and a Gynandromorph of Megalopta genalis (Hymenoptera:

Halictidae). J. Nat. Hist. 38: 1443–1457. http://dx.doi.org/10.1080/0022293031000155322.

Wiens, J.J. (2001). Character Analysis in Morphological Phylogenetics: Problems and Solutions. Syst. Biol. 50: 689-699. http://dx.doi.org/10.1080/106351501753328811.

Wetterer, J.K. & Porter, S.D. (2003). The Llittle Fire Ant, Wasmannia auropunctata: Distribution, Impact, and Control. Sociobiology 42: 1-41.




How to Cite

Cuezzo, F. del C., Calcaterra, L., Chifflet, L., & Follet, P. (2015). Wasmannia Forel (Hymenoptera: Formicidae: Myrmicinae) in Argentina: Systematics and Distribution. Sociobiology, 62(2), 246–265. https://doi.org/10.13102/sociobiology.v62i2.246-265



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