Determination of acute lethal doses (LD50 and LC50) of imidacloprid for the native bee Melipona scutellaris Latreille, 1811 (Hymenoptera: Apidae)

Authors

  • Leticia Mariano da Costa Federal University of São Carlos
  • Tatiane Caroline Grella Federal University of São Carlos
  • Rodrigo Avelaira Barbosa São Paulo State University
  • Osmar Malaspina São Paulo State University
  • Roberta Cornélio Ferreira Nocelli Federal University of São Carlos

DOI:

https://doi.org/10.13102/sociobiology.v62i4.792

Keywords:

stingless bees, insect, neonicotinoid, toxicity

Abstract

The bee species Melipona scutellaris (L.) is native to Brazil, stingless and belongs to the Apidae family. In Brazil, stingless bees are responsible for 40 % to 90% of tree species pollination, depending on the considered ecosystem. However, their survival has been threatened since the country has been standing out as the biggest pesticides consumer in the world. Many of the pesticides used are considered toxic to bees, including imidacloprid (all existing studies were conducted with the Apis mellifera (L.) species). Although the bees are not the target of these substances, they are highly vulnerable to contamination. Thereby, the objective of this study was to establish the mean lethal dose (LD50) and the mean lethal concentration (LC50) of imidacloprid in the M. scutellaris fodder. In order to carry out this experiment, bees were collected and the test was performed according to OECD's protocol (1998a, 1998b), developed for A. mellifera. For the determination of LD50 and LC50, data was analyzed through the Probit method, using the BioStat software. The topical LD50 established in this study was 2.41 ng/bee for 24 hours and 1.29 ng/bee for 48 hours. The oral LC50 was 2.01 ng i.a./μL for 24 hours and 0.81 ng i.a./μL for 48 hours. This data showed that native bees of M. scutellaris species are more sensitive to this pesticide than A. mellifera. Thus, it is important to establish management methods which take this higher susceptibility into account to protect native species.

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References

Ballivián, J.M.P.P. (Org.). (2008). Abelhas nativas sem ferrão – Myg Pe – Indiginous Land, Guarita, RS. São Leopoldo: Oikos, 128p.

Decourtye, A., Devillers, J., Genecque, E., Le Menach, K., Budzinski, H., Cluzeau, S. & Pham-Delègue, M.H. (2005).

Comparative Sublethal Toxicity of Nine Pesticides on Olfactory Learning Performances of the Honeybee Apis mellifera. Archives of Environmental Contamination and Toxicology, 48: 242-250. doi: 10.1007/s00244-003-0262-7.

Del Sarto, M.C.L., Oliveira, E.E., Guedes, R.N.C. & Campos, L.A.O. (2014). Differential insecticide susceptibility of the neotropical stingless bee Melipona quadrifasciata and the honey bee Apis mellifera. Apidologie, 45: 626-636. doi: 10.1007/s13592-014-0281-6.

Finney, D.J. (1952). Probit analysis. Cambridge: University Press, 318 p. Imperatriz-Fonseca, V.L. & Santos, I.A. (2014). Meliponineos. http://www.webbee.org.br/beetaxon/. (access date: August 22th, 2014). Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis - IBAMA. (2012). Efeito dos agrotóxicos sobre as abelhas silvestres no Brasil. Brasília, 88p.

Iwasa, T., Motoyama, N., Ambrose, J.T. & Roe, R.M. (2004). Mechanism for the differential toxicity of neonicotinoid insecticides in the honeybee, Apis mellifera. Crop Protection, 5: 371-378. doi: 10.1016/j.cropro.2003.08.018.

Jacob, C.R.O., Soares, H.M., Carvalho, S.M., Nocelli, R.C.F. & Malaspina, O. (2013). Acute Toxicity of Fpronil to the Stingless Bee Scaptotrigona postica Latreille. Bulletin of Environmental Contamination and Toxicology, 90: 69-72. doi: 10.1007/s00128-012-0892-4.

Johansen, C.A. & Mayer, D.F. (1990). Pollinator protection: a bee and pesticide handbook. Cheshire: Wicwas Pr, 212 p.

Kerr, W.E., Carvalho, G.A., Silva, A.C. & Assis, M.G.P. (2001). Aspectos pouco mencionados da biodiversidade amazônica. Strategic Partnerships, 12: 20-41.

Lourenço, C.T., Carvalho, S.M., Malaspina, O. & Nocelli, R.C.F. (2012a). Determination of fpronil LD50 for the Brazilian bee Melipona scutellaris. Julius Kühn-Institut., 437: 174-178. doi: 10.5073/jka.2012.437.046.

Lourenço, C.T., Carvalho, S.M., Malaspina, O. & Nocelli, R.C.F. (2012b). Oral Toxicity of Fipronil Insecticide Against the Stingless Bee Melipona scutellaris (Latreille, 1811). Bulletin of Environmental Contamination and Toxicology, 89: 921-924. doi: 10.1007/s00128-012-0773-x.

Moraes, S.S., Bautista, A.R.L. & Viana, B.F. (2000). Avaliação da toxicidade aguda (DL50 e CL50) de inseticidas para Scaptotrigona tubiba (Smith) (Hymenoptera: Apidae): Via de Contato. Anais da Sociedade Entomologica do Brasil, 1: 31-37. doi: 10.1590/S0301-80592000000100004.

National Pesticide Information Center - NPIC. (2010). Imidacloprid: technical fact sheet. http://npic.orst.edu/ factsheets/fiptech.pdf. (access date: March 7th, 2015).

Nauen, R., Ebbinghaus-Kintscher, U. & Schmuck, R. (2001). Toxicity and nicotinic acetylcholine receptor interaction of imidacloprid and its metabolites in Apis mellifera (Hymenoptera: Apidae). Pest Management Science, 7: 577- 586. doi: 10.1002/ps.331

Nogueira-Neto, P. (1997). Vida e criação de abelhas indígenas sem ferrão. São Paulo: Nogueirapis, 445 p.

OECD Guidelines For The Testing Of Chemicals, Section 2, Effects On Biotic Systems. Honeybees, Acute Contact Toxicity Test, n.214, set. 1998a. 7p.

OECD Guidelines For The Testing Of Chemicals, Section 2, Effects On Biotic Systems. Honeybees, Acute Oral Toxicity Test, n.213. set. 1998b, 8p.

Ramalho, M. & Batista, M.A. (2005). Polinização na Mata Atlântica: perspectiva ecológica da fragmentação. In FRANKE, C. R. et al., Mata Atlântica e biodiversidade (pp. 93-142). Salvador: EDUFBA.

Schmuck R., Schoning R., Stork A. & Schramel O. (2001) Risk posed to honeybees (Apis mellifera L, Hymenoptera) by an imidacloprid seed dressing of sunflowers. Pest Management Science, 57: 225-238. doi:10.1002/ps.270

Schmuck, R., Nauen, R. & Ebbinghaus-Kintscher, U. (2003). Effects of imidacloprid and common plant metabolites of imidacloprid in the honeybee: toxicological and biochemical considerations. Bulletin of. Insectology, 56: 27-34.

Soares, H.M., Jacob, C.R.O., Carvalho, S.M., Nocelli, R.C.F. & Malaspina, O. (2015). Toxicity of imidacloprid to the stingless bee Scaptotrigona postica Latreille, 1807 (Hymenoptera: Apidae). Bulletin of Environmental Contamination and Toxicology, 94: 675-680. doi: 10.1007/ s00128-015-1488-6

Suchail, S., Debrauwer, L. & Belzunces, L.P. (2003). Metabolism of imidacloprid in Apis mellifera. Pest Management Science, 3: 291-296. doi: 10.1002/ps.772

Suchail, S., Guez, D. & Belzunces, L.P. (2000). Characteristics of imidacloprid toxicity in two Apis mellifera subspecies. Environmental Toxicology and Chemistry, 7: 1901-1905. doi: 10.1002/etc.5620190726.

Suchail, S., Guez, D. & Belzunces, L.P. (2001). Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environmental Toxicology and Chemistry, 20: 2482-2486.

Tingle, C.C., Rother, J.A., Dewhurst, C.F., Lauer, S. & King, W.J. (2003). Fipronil environmental fate, ecotoxicology and human health concerns. Rev. Environ. Contam. Toxicol., 176: 1-66. doi: 10.1007/978-1-4899-7283-5_1

Tomizawa, M. & Casida, J.E. (2003). Selective toxicity of neonicotinoids attributable to specificity of insect and mammalian nicotinic receptors. Annual Review of Entomology, 48: 339-364. doi: 10.1146/annurev. ento.48.091801.112731.

Valdovinos-Núñez, G.R., Quezada-Euán, J.J.G., AnconaXiu, P., Moo-Valle, H., Carmona, A. & Sánchez, E.R. (2009). Comparative toxicity of pesticides to stingless bees (Hymenoptera: Apidae: Meliponini). Journal of Economic Entomology, 102: 1737-1742. doi: http://dx.doi. org/10.1603/029.102.0502.

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Published

2015-12-30

How to Cite

Costa, L. M. da, Grella, T. C., Barbosa, R. A., Malaspina, O., & Nocelli, R. C. F. (2015). Determination of acute lethal doses (LD50 and LC50) of imidacloprid for the native bee Melipona scutellaris Latreille, 1811 (Hymenoptera: Apidae). Sociobiology, 62(4), 578–582. https://doi.org/10.13102/sociobiology.v62i4.792

Issue

Vol. 62 No. 4 (2015)

Section

Research Article - Bees

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