Male Orchid Bees (Hymenoptera: Apidae: Euglossini) in Canopy and Understory of Amazon Várzea Floodplain Forest. I. Microclimatic, Seasonal and Faunal Aspects

Patricia dos Santos Vilhena


Várzea floodplain forests are important ecosystems of the Amazon basin. Our goal was to verify whether orchid bee males have stratum preference in a forest with a dynamic understory. Traps were placed at 25 m and 1.5 m with aromatic scents to lure the bees. Data loggers were used to register temperature and relative humidity in both heights. Of 835 sampled males belonging to 28 species, 645 males from 23 species were collected at the understory. Temperature was 1.4º C higher at the canopy and relative humidity was 11.58% higher at the understory. Temperature, relative humidity, wind and tidal levels were the most important abiotic variables influencing mainly the canopy assemblage. The understory is a more stable environment, presenting good conditions to survival of the males. The species Euglossa cognata Moure, Eulaema meriana Olivier and Euglossa ignita Smith were closely related to strata and seasons.


Amapá; vertical stratification; euglossine bees; Euglossini

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Abrahamczyk, S., Gottleuber, P., Matauschek, C. & Kessler, M. (2011). Diversity and community composition of euglossine bee assemblages (Hymenoptera: Apidae) in western Amazonia. Biodiversity and Conservation, 20: 2981-3001. doi: 10.1007/s10531-011-0105-1

Ackerman, J.D. (1983) Diversity and seasonality of male euglossine bees (Hymenoptera: Apidae) in Central Panamá. Ecology, 64: 274-283. doi: 10.2307/1937075

Bawa, K.S., Bullock, S.H., Perry, D.R., Coville, R.E. & Grayum, M.H. (1985). Reproductive Biology of Tropical Lowland Rain Forest Trees. II. Pollination Systems. American Journal of Botany, 72: 346-356.

Cameron, S.A. & Ramírez, S. (2001). Nest architecture and nest ecology of the orchid bee Eulaema meriana (Hymenoptera: Apidae: Euglossini). Journal of the Kansas Entomologycal Society, 74: 142-165.

Casey, T.M., May, M.L. & Morgan, K.R. (1985). Flights energetics of euglossinae bees in relation to morphology and wing stroke frequency. Journal of Experimental Biology, 116: 571-289.

Dressler, R.L. (1982). Biology of the orchid bees (Euglossini). Annual Review of Ecology and Systematics, 13:373-94. doi: 10.1146/

Drummond, J.A., Dias, T.C.A. C. & Brito, D.M.C. (2008). Atlas das Unidades de Conservação do Estado do Amapá. GEA/SEMA, MMA/IBAMA-AP, Macapá. 128pp.

Dudley, N. (2008). Guidelines for Applying Protected Area Management Categories. Gland, Switzerland. 86 pp.

Eltz, T., Roubik, D.W., Whitten, W.M. (2003). Fragrances, male display and mating behaviour of Euglossa hemichlora: a flight cage experiment. Physiological Entomology 28: 251-260. doi: 10.1111/j.1365-3032.2003.00340.x

Erwin, T.L. (1982). Tropical Forest: Their richness in Coleoptera and other arthopods species. The Coleopterists Bulletin, 36: 74-75.

Ferreira, M.G., Pinho, O.C., Balestieri, J.B.P. & Faccenda, O. (2011). Fauna and Stratification of Male Orchid Bees (Hymenoptera: Apidae) and their Preference for Odor Baits in a Forest Fragment. Neotropical Entomolgy, 40: 639-646. doi: 10.1590/S1519-566X2011000600002

Gama, J.R.V., Souza, A.L, Martins, S.V. & Souza, D.R. (2005). Comparação entre florestas de várzea e de terra firme do Estado do Pará. Revista Árvore, 29: 607-161. doi: 10.1590/S0100-67622005000400013

Garófalo, C.A., Camillo, E., Serrano, J.C. & Rebêlo, J.M.M. (1993). Utilization of trap-nests by Euglossini species (Hymenoptera: Apidae). Revista Brasileira de Biologia, 53: 177-187.

Giovanni, F.D., Cerretti, P., Mason, F., Minari, E. & Marini, L. (2015). Vertical stratification of ichneumonid wasp communities: the effects of forest structure and life-history traits. Insect Science, 22: 688-699. doi: 10.1111/1744-7917.12153

Hiura, A.L., Sarquis, R.S.F.R. (2008). Características morfológicas de espécies arbóreas na floresta de várzea da APA da Fazendinha, Macapá/AP. Revista Pesquisa e Iniciação Científica – Amapá, 4º Sessão: Botânica Estrutural, 1: 35-39.

Inouye, D.W. (1975) Flight temperature of male euglossine bees. Journal of the Kansas Entomologycal Society, 48: 366- 370.

Kamke, R., Zillikens, A., Heinle, S. & Steine, J. (2008). Natural Enemies and Life Cycle of The Orchid Bee Eufriesea smaragdina (Hymenoptera: Apidae) Reared from Trap Nests. Journal of the Kansas Entomological Society, 81: 101-109.

Magurran, A.E. (2004) Measuring biological diversity. Oxford: Blackwell Publishing, USA. 256 p.

Martins, C.F.& Souza, A.K.P. (2005). Estratificação vertical de abelhas Euglossini (Hymenoptera, Apidae) em uma área de Mata Atlântica, Paraíba, Brasil. Revista Brasileira de Zoologia, 22: 913-918. doi: 10.1590/S0101-81752005000400016

Mateus, S., Andrade-Silva, A.C.R. & Garófalo, C.A. (2015). Diversity and Temporal Variation in the Orchid Bees Community (Hymenoptera: Apidae) of a Remnant of a Neotropical Seasonal Semi-deciduous Forest. Sociobiology, 62: 571-577. doi: 10.13102/sociobiology.v62i4.391

May, M.L. & Casey, T.M. (1983). Thermoregulation and heat exchange in euglossine bees. Physiological Zoology, 56: 541-551.

Olesen, J.M. (1988). Nest structure of a Euglossa sp. n. in a fruit of Theobroma subincanum from Ecuadorean Amazonas. Acta Amazonica, 183: 327-330. doi: 10.1590/1809-43921988183330

Oliveira, M.L. & Campos, L.A.O. (1995). Abundância, riqueza e diversidade de abelhas Euglossinae (hymenoptera, apidae) em florestas contínuas de terra firme na Amazônia Central, Brasil. Revista Brasileira de Zoologia, 13: 547-556. doi:

Oliveira, M.L. & Campos, L.A.O. (1996). Preferência por estratos florestais e por substâncias odoríferas em abelhas Euglossini (Hymenoptera, Apidae). Revista Brasileira de Zoologia, 13: 1075-1085.

Oliveira, M.L. (1999). Sazonalidade e horário de atividade de abelhas Euglossinae (Hymenoptera, Apidae) em florestas de terra firme na Amazônia Central. Revista Brasileira de Zoologia, 16: 83-90.

Oliveira, R., Pinto, E.C. & Schlindwein, C. (2015). Two common species dominate the species-rich Euglossine bee fauna of an Atlantic Rainforest remnant in Pernambuco, Brazil. Brazilian Journal of Biology, 75(4): S1-S8. doi: 10.1590/1519-6984.18513

Otero, J.T. & Sallenave, A. (2003). Vertical stratification of euglossine bees (Hymenoptera: Apidae) in an Amazonian forest. The Pan-Pacific Entomologist, 79: 151-154.

Peel, M.C., Finlayson, B.L. & McMahon, T.A. (2007). Updated world map of the Koppen-Geiger climate classification. Hydrology and Earth System Sciences, 11: 1633–1644.

Peruquetti, R.C. & Campos, L.A. (1997). Aspectos da biologia de Euplusia violacea (Blanchard) (Hymenoptera, Apidae, Euglossini). Revista Brasileira de Zoologia, 14(1): 91-97. doi: 10.1590/S0101-81751997000100009

Piedade, M.T.F., Worbes, M. & Junk, W.J. (2001). Geo-ecological controls on elemental fluxes in communities of higher plants in Amazonian floodplains, in: McClain, M.E.; Victoria, R. L., Richey, J. E. (Eds). The Biogeochemistry of the Amazon Basin. Oxford University Press, New York, pp. 209-234.

Pinedo-Vasquez, M., Ruffino, M.L., Padoch, C., & Brondízio, E.S. (2010). The Amazon Várzea. The decade past and the decade ahead. Springer, London. 528 p.

Prance, G.T. (1979). Notes on the vegetation of Amazonia III. The terminology of Amazonian forest types subject to inundation. Brittonia, 3: 26–38. doi: 10.2307/2806669

Rebêlo, J.M.M. & Garófalo, C.A. (1997). Comunidades de machos de Euglossini (Hymenoptera: Apidae) em matas semidecíduas do nordeste do estado de São Paulo. Anais da Sociedade Entomológica do Brasil, 26: 243-255. doi: 0.1590/S0301-80591997000200005

Rocha-Filho, L.C. & Garófalo, C.A. (2014). Phenological Patterns and Preferences for Aromatic Compounds by Male Euglossine Bees (Hymenoptera, Apidae) in Two Coastal Ecosystems of the Brazilian Atlantic Forest. Neotropical Entomology, 43:9–20. doi: 10.1007/s13592-014-0322-1

Roubik, D.W. (1993). Tropical pollinators in the canopy and understory – Field data and teory of stratum preferences. Journal of Insect Behavior 6: 659-673. doi: 10.1007/BF01201668

Santos, M.L. & Garófalo, C.A. (1994). Nesting biology and nest re-use of Eulaema nigrita (Hymenoptera: Apidae: Euglossini). Insectes Sociaux, 41: 99-110.

Sobek, S.,Tscharntke, T., Scherber, C., Schiele, S. & Steffan-Dewenter, I. (2009). Canopy vs. understory: Does tree diversity affect bee and wasp communities and their natural enemies across forest strata? Forest Ecology and Management, 258: 609-615. doi: 10.1016/j.foreco.2009.04.026

Stangler, E.S., Hanson, P.E. & Steffan-Dewenter, I. (2016). Vertical diversity patterns and biotic interactions of trap-nesting bees along a fragmentation gradient of small secondary rainforest remnants. Apidologie, 47: 527-538. doi: 10.1007/s13592-015-0397-3

Stern, D.L. (1991). Male territoriality and alternative male behaviors in the euglossine bee, Eulaema meriana (Hymenoptera: Apidae). Journal of the Kansas Entomological Society, 64: 421-437.

Storck-Tonon, D., Morato, E.F. & Oliveira, M.L. (2009). Fauna de Euglossini (Hymenoptera: Apidae) da Amazônia Sul-Ocidental, Acre, Brasil. Acta Amazonica, 39: 693-706. doi: 10.1590/S0044-59672009000300026

Stork, N.E. & Grimbacher, P.S. (2006). Beetle assemblages from an Australian tropical rainforest show that the canopy and the ground strata contribute equally to biodiversity. Proceedings of the Royal Society B, 273: 1969–1975. doi: 10.1098/rspb.2006.3521

Tilman, D. (1990). Constraints and tradeoffs: toward a predictive theory of competition and succession. Oikos, 58: 3-15. doi: 10.2307/3565355

Ulyshen, M.D. (2011). Arthropod vertical stratification in temperate deciduous forests: implications for conservation-oriented management. Forest Ecology and Management, 261: 1479–1489. doi: 10.1016/j.foreco.2011.01.033

Ulyshen, M.D., Soon, V. & Hanula, J.L. (2010). On the vertical distribution of bees in a temperate deciduous forest. Insect Conservation and Diversity, 3: 222-228. doi: 10.1111/j.1752-4598.2010.00092.x

Zar, J.H. (2014). Biostatistical analysis. Fifty Edition. Pearson, England. 960 p.

Zucchi, R., Sakagami, S.F. & Camargo, J.M.F. (1969). Biological observations on a Neotropical parasocial bee, Eulaema nigrita, with a review of the biology of Euglossinae (Hymenoptera, Apidae): a comparative study. Journal of the Faculty of Sciences of the Hokkaido University, 17: 271-380.



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