Development and Isolation of 17 polymorphic Microsatellite Loci in Coptoterms formosanus (Isoptera: Rhinotermitidae)

Zhi-Qiang Li

Abstract


Seventeen polymorphic microsatellite DNA loci for Copototermes formosanus were isolated and characterized. Polymorphism of these loci was assessed in a sample of 32 unrelated C. formosanus individuals. An average of 4.6 alleles per locus (3-8 alleles) was detected. Observed and expected heterozygosities ranged from 0.2500 to 1.0000 and from 0.5591 to 0.8562, respectively. Six loci were found to have deviated from Hardy-Weinberg equilibrium in the sampled population after Bonferroni correction. No significant linkage disequilibrium was detected. These markers will be useful in population genetics, phylogenies and other relevant studies of C. formosanus.


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Austin, J.W., et al. 2006. Genetic evidence for two introductions of the Formosan subterranean termite, Coptotermes formosanus (Isoptera:Rhinotermitidae), to the United States. Florida Entomologist 89:183-193.

Fang, R., L. Huang & J.H. Zhong 2008. Surprising low levels of genetic diversity offormosanu subterranean termites in south China as revealed by the COII gene (Isoptera: Rhinotermitidae) sociobiology 51(1): 1-20.

Jenkins, T.M., R.E. Dean & B.T. Forschler 2002. DNA technology, interstate commerce, and the likely origin of formosan subterranean termite (Isoptera: Rhinotermitidae) infestation in Atlanta, Georgia. J. Econ. Entomol. 95:381-389.

Raymond, M., F. Rousset 1995. Genepop (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248-249.

Ross, K.G. 2001. Molecular ecology of social behaviour: analyses of breeding systems and genetic structure. Mol. Ecol. 10: 265-284.

Su, N.Y, M. Tamashiro 1987. An overview of the Formosan subterranean termite (Isoptera: Rhinotermitidae) in the world. In: Biology and Control of the Formosan Subterranean Termite. College of Agriculture and Human Resources, 3-15.

Vargo, E.L. & G. Henderson 2000. Identification of polymorphic microsatellite loci in the Formosan subterranean termite Coptotermes fomosanus Shiraki. Mol. Ecol. 9:1935–1938.

Vargo, E.L., C. Husseneder & J.K. Grace 2003. Colony and population genetic structure of the Formosan subterranean termite, Coptotermes formosanus, in Japan. Mol. Ecol. 12: 2599-2608.

Varshney, R.K., A. Graner & M.E. Sorrells 2005. Genetic microsatellite markers in plants: features and applications. Trends Biotechnol. 23:48–55.

Yu, D.M., B. Ma, Y.H. Sun, & J.J. Peng 2010. Isolation and characterization of 16 microsatellite loci in an endangered fish Ussuri cisco, Coregonus usssruensis. Conserv Genet 11:1107-1109.

Zhang, D.X. & G.M. Hewitt 1998. Isolation of animal cellular total DNA. Molecular Tools for Screening Biodiversity (eds. A. Karp, P.G. Isaac & D.S. Ingram), Chapman & Hall, London, pp. 5-9.




DOI: http://dx.doi.org/10.13102/sociobiology.v59i4.494

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