Molecular Cloning and Expression of an Estrogen Receptor-Related Receptor Gene in Apis mellifera (Hymenoptera:Apidae)

Gengsi Xi


Estrogen receptor-related receptors (ERRs) belong to a subfamily of orphan nuclear receptors where the proteins are closely related to the estrogen receptors (ERs) in structure. ERR homologs have been found in many animals and play an important role in the regulation of physiologic processes. In our study, the estrogen receptor-related receptor homolog gene (referred to as AmERR) was cloned from Apis mellifera Linnaeus. The full-length cDNA of the AmERR gene is 1779 bp, containing a 5’- untranslated region (5’- UTR) of 197 bp and a 3’-UTR of 277 bp. The open reading frame of 1,305 bp encodes a 434-amino acid protein. Using real-time quantitative RT-PCR to study AmERR mRNA expression patterns indicated that this gene is differentially expressed during honeybee development. There is a remarkable increase of relative expression of AmERR mRNA from pupa to adult , which may indicate that the gene plays an important role in the bee’s differentiation of tissues and development. A different expression in castes and abundance expression in adults of AmERR mRNA may suggest it is relevant to functions in social communities.


molecular cloning; estrogen receptor-related receptors(ERR); Apis mellifera; real-time quantitative RT-PCR

Full Text:



Cheung, C.P., S. Yu, K.B. Wong, L.W. Chan, F.M. Lai, X.H. Wang, M. Suetsugi, S. Chen & F.L. Chan 2005. Expression and functional study of estrogen receptor-related receptors in human prostatic cells and tissues. The Journal of Clinical Endocrinology and Metabolism 90:1830-1844.

Claeys, K.G., G.A. Orban, P. Dupont, S. Sunaert, P.V. Hecke & E.D. Schutter 2003. Involvement of multiple functionally distinct cerebellar regions in visual discrimination: a human functional imaging study. Neuroimage 20:840–854.

Escriva, H., F. Delaunay & V. Laudet 2000. Ligand binding and nuclear receptor evolution. Bioessays 22:717-727.

Fitzgerald, M. & T. Shenk 1981.The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs. Cell 24(1): 251-260

Giguere, V. 2002. To ERR in the estrogen pathway. Trends in Endocrinology and Metabolism 13: 220-225.

Giguere, V., N. Yang, P. Segui & R.M. Evans 1988. Identification of a new class of steroid hormone receptors. Nature 331: 91-94.

Guo, X.J. & G.S. Xi 2010. Molecular Cloning and Expression Analysis of the Gene Encoding MEF2 in the Ant Polyrhachis vicina (Hymenoptera:Formicidae). Sociobiology 56(1):235-248

Jeanmougin, F., J.D. Thompson, M. Gouy, D.G. Higgins & T.J. Gibson 1998. Multiple sequence alignment with Clustal X. Trends in Biochemical Science 23: 403-405.

Kraus, R.J., E.A. Ariazi, M.L. Farrell & J.E. Mertz 2002.Estrogen-related receptor alpha 1 actively antagonizes estrogen receptor-regulated transcription in MCF-7 mammary cells. The Journal of Biological Chemistry 277:24826-24834.

Lü, S. M., G.S. Xi & X.H. Wang 2008.Molecular cloning, characterization, and expression analysis of a QM homologue in the ant Polyrhachis vicina (Hymenoptera:Formicidae). The Canadian Entomologist 140:312-323.

Ouyang, X.H., G.S. Xi & C.P. Bu 2009. Molecular cloning and expression of an estrogen receptor-related receptor gene in the ant Polyrhachis vicina (Hymenoptera: Formicidae). Annals of the Entomological Society of America 102(2):295-302.

Pettersson, K., K. Svensson, R. Mattsson, B. Carlsson, R. Ohlsson & A. Berkenstam 1996. Expression of a novel member of estrogen response element-binding nuclear receptors is restricted to the early stages of chorion formation during mouse embryogenesis. Mechanisms of Development 54:211-223.

Simonet, G., J. Poels, I. Claeys, T.V. Loy, V. Franssens, A.D. Loof & J.V. Broeck 2004. Neuroendocrinological and molecular aspects of insect reproduction. Journal of Neuroendocrinology 16:649 -659.

SPSS Inc. 2004. SPSS version 13.0.SPSS Inc., Chicago.

Tamura, K., J. Dudley, M. Nei & S. Kumar 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596- 1599.

Vanacker, J.M., K. Petterson, J.A. Gustafsson & V. Laudet 1999a. Transcriptional activities of the orphan nuclear receptor ERR alpha (estrogen receptor-related receptor-alpha). Molecular Endocrinology 13: 764-773.

Vanacker, J.M., K. Petterson, J.A. Gustafsson & V. Laudet 1999b. Transcriptional targets shared by estrogen receptor-related receptors(ERRs) and estrogen receptor (ER)α, but not by (ER) β. The EMBO Journal 18: 4270-4279.

Yang, N., H. Shigeta, H. Shi & C.T. Teng 1996. Estrogen-related receptor, HERR1,modulates estrogen receptor-mediated response of human anlactoferrin gene promoter. The Journal of Biological Chemistry 271:5795-5804.

Zhang, Z. & C.T. Teng 2000. Estrogen receptor-related receptor alpha 1 interacts with coactivator and constitutively activates the estrogen response elements of the human lactoferrin gene. The Journal of Biological Chemistry 275:20837-20846.



  • There are currently no refbacks.

JCR Impact Factor 2018: 0.504