Risk of local extinction and genetic diversity of Melipona quadrifasciata (Apidae: Meliponini) in a possible Northeastern limit of its distribution in Brazil

Edilson D Araújo, Rosane Gomes Oliveira, Higor Cesar Meneses Calazans, Carina Caroline Silva França, Valdson SANTOS, Sona Arun Jain, Marcus Vinícius Aragão Batista, Lorena Andrade Nunes, Genésio Tamara Ribeiro

Abstract


Rapid loss of genetic diversity among eusocial bees, and extinction of their local population has become a major world concern. Populations of M. quadrifasciata have increasingly declined due to predatory extractivism and destruction of their habitat. Knowledge of their local population could give insights on the strategies for monitoring and conservation of this species. In this study, initially, 14 colonies (140 workers) from the Northern limit of the Northeastern sandbank of the Atlantic Forest were analyzed employing geometric morphometrics techniques. Then the cytochrome b gene sequences and ISSR primers were utilized for molecular analysis. The results were compared with the results of 30 M. quadrifasciata colonies (300 workers) found in the semiarid Caatinga region. The morphometrics and the molecular analysis suggested the formation of disjunct populations between the studied geographical zones. All the 14 colonies analyzed from the river São Francisco region were found on the Southern margin of the river São Francisco and they all belonged to one single haplotype. No colonies were found on the Northern bank of the river. We suggest that the river São Francisco is an effective georgraphical barrier for the distribution of this species and propose an urgent need for the development of a conservation program for the population of M. quadrifasciata as it represents a unique haplotype in the region. Also their nesting habit exclusively in the diseased coconut trees which probably will be replaced by new trees, create the risk of population extinction due to the lack of nesting site.


Keywords


Coconut trees; nesting site; geometric morphometrics; stingless bee; molecular marker

Full Text:

PDF

References


Antonini, Y., Costa, R. G., Martins, R. P. (2006). Floral preferences of a neotropical stingless bee, Melipona quadrifasciata Lepeletier (Apidae: Meliponina) in a urban forest fragment. Revista Brasileira de Biologia, 66 (2A): 463-471. doi:10.1590/S1519-69842006000300012.

Araújo, E.D., Costa, M., Chaud-Neto, J., Fowler, H.G. (2004). Body size and flight distance in stingless bees (Hymenoptera: Meliponini): Interference of flight range and possible ecological implications. Brazilian Journal of Biology, 64: 563-368. doi:10.1590/S1519-69842004000400003.

Araújo, E.D., Diniz-Filho, J.A.F., Oliveira, F.A. (2000). Extinção de populações locais do gênero Melipona (Hymenoptera: Meliponinae):efeito do tamanho populacional e da produção de machos por operárias. Naturalia, 25: 287-299.

Batalha-Filho, H., Melo, G.A.R., Waldschmidt, A.M., Campos, L.A.O., Fernandes-Salomão, T.M. (2009). Geographic distribution and spatial differentiation in the color pattern of abdominal stripes of the Neotropical stingless bee Melipona quadrifasciata (Hymenoptera, Apidae). Zoologia (Curitiba, Impresso), 26: 13-219. doi:10.1590/S1984-46702009000200003.

Batista, M.A., Ramalho, M., Soares, E.E.A. (2003). Nesting sites and abundance of Meliponini (Hymenoptera: Apidae) in heterogeneous habitats of the Atlantic Rain Forest, Bahia, Brazil. Lundiana, 4: 19-23.

Bandelt, H.J., Forster, P., Rohl, A. (1999). Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16: 37-48.

Beaumont, M.A., Bruford, M.W. (1999). Microssatélites in conservation genetics. In: Microssatélites: Evolution and Aplications (eds. D.B. Goldstein e C. schotterer), Oxf Univ Press, New York, pp.165-182.

Camargo, J.M.F., Pedro, S.M.R. (2007). Meliponini Lepelitier, 1836 pp272-578. In: JS Moure, DUR Bane GAR Melo (EDs) Catalogue off bee (Hymenoptera, Apoidae) in the Neotropical Region. Curitiba, Revista da Sociedade Brasileira de Entomologia, 21: 1558. doi:10.1590/s0101-81752002000500001.

Carvalho, G.A., Kerr, W., Nascimento, V.A. (1995). Sex determination in bees. XXXVII. Decrease of Xo hetero alleles in a finite population of Melipona scutellaris. (Apidae, Meliponini). Brazilian Journal of Genetics, 18: 13-16.

Chakrabortyr, R., Kimmel, M. (1999). Statistics of microssatélites loci: estimation of mutation rate and pattern of population expansion. “In” Microssatélites: Evolution and applications” (eds. D.B.Goldstein e C. schotterer), Oxf Univ Press, New York, pp.139-150.

Chen, K., Kinorr, C., Bornemann-Kolatzki, K., Ren, J., Huang, L., Rohrer, G.A., Brenig, B. (2005). Targeted oligonucleotide-mediated microsatellite identificatio (TOMMI) from large-insert library clones. BMC Genetics, 6: 54. doi:10.1186/1471-2156-6-54.

Crozier, Y.C., Koulianos, S., Crozier, R.H. (1991). An improved test for Africanized honey bee mitochondrial DNA. Experientia, 47: 968-969. doi:10.1007/BF01929894

Cuenca, M.A.G., Nazario, C.C. (2004). Evolução da cocoicultura no Estado e nos tabuleiros costeiros do Rio Grande do Norte no periodo de 1990 a 2002. Aracaju: Embrapa Tabuleiros Costeiros.

Diniz, F.M., Maclean, N., Paterson, I.G., Bentzen, P. (2004). Polymorphic tetranucleotide microsatellite markers in the Caribbean spiny lobster, Panulirus argus. Molecular and Ecology, 4: 327-329. doi:10.1111/j.1471-8286.2004.00683.x.

Diniz, F.M., Maclean, N., Ogawa, M., Paterson, I.G., Bentzen, P. (2005). Microsatellites in the overexploited spiny lobster, Panulirus argus : Isolation, characterization of loci and potential for intraspecific variability studies. Conservation Genetics, 6: 637-641. doi:10.1007/s10592-005-9018-4.

Evanno, G., Regnaut, S., Goudet, J. (2005). Detecting the number of clusters of individuals usingthe software STRUCTURE: a simulation study. Molecular and Ecology, 14: 2611-2620.

Excoffier, L., Laval, G., Schneider, S. (2005). Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics, 1: 47-50.

Hammer, O., Harper, D.A.T., Ryan, P.D. (2001). Past: Paleontological Statistics Software Package for Education and Data Analysis. Paleontologia Electronica, 4: 1-9.

Kerr, W.E., Vencovsky, R.(1982). Melhoramento genético em abelhas I. Efeito do número de colônias sobre o melhoramento. Revista Brasileira de Genetica, 5: 279-285.

Kerr, E.W., Carvalho, G.A., Nascimento, V.A. (1996). Abelha uruçú: Biologia, Manejo e Conservação. Editora Liber Liber, Belo Horizonte.

Klingenberg, P.C. (2010). Morphoj: an integrated software package for geometric Morphometrics. Molecular and Ecology Resources, 11: 353-357. doi:10.1111/j.1755-0998.2010.02924.x.

Librado, P., Rozas, J. (2009). DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25: 1451-1452. doi:10.1093/bioinformatics/btp187

Matioli, S.R., Passo-Bueno, M.R.S. (2001). Métodos baseados em PCR para análise de Polimorfismos de ácidos nucléicos. In: MATIOLI, S. R. (ed). Molecular Biology and Evolution, Holos, pp.153-161.

Mirisola -Filho, L.A. (2002). Cultivo de Coco Anão. Viçosa: Aprenda Fácil, pp344

Moritz, C., & Hills, D.M. (1996). Molecular systematics: context and controversies. “ In” Mol systematic. 2nd edition (eds.D.M. Hills; C. Moritz e B.K. Mable), sinauer Associates, Massachuttes. pp.1-13.

Nascimento, M.A., Batalha-Filho, H., Waldschmidt, A.M., Tavares, M.G., Campos, L.A.O., Fernandes-Salomão, T.M. (2010). Variation and genetic structure of Melipona quadrifasciata Lepeletier. Genetics Molecular and Biology, 33: 394–397. doi:10.1590/S1415-47572010005000052.

Ramalho, M. (2004). Stingless bees and mass flowering trees in the canopy of Atlantic Forest: a tight relationship. Acta Botanica Brasilica, 18: 37-47.doi:10.1590/S0102-33062004000100005

Ribeiro, M.C., Metzger, J. P., Martensen, A.M., Ponzoni, F.J., Hirota, M.M.(2009). The Brazilian Atlantic Forest: How much is left, and how is the remaining forest distributed: Implications for conservation. Biological Conservation, 142: 1141–1153. doi:10.1007/978-3-642-20992-5_21.

Rolfh, F.J. (2000). NTSYS-pc: numerical taxonomy and multivariate analysis system,

version 2.1. Exeter Software: State University of New York, Setauket.

Silveira, F.A., Melo, G.A.R., Almeida, E.A.B. (2002). Abelhas brasileiras: sistemática e identificação. Fundação Araucária, Belo Horizonte.

Siqueira, L.A., Aragão, W.M., Tupinambá, L.A. (2002). A introdução do coqueiro no Brasil, importância histórica e agronômica. 24p. (Embrapa Tabuleiros Costeiros). Documentos, 47. http://www.cpatc.embrapa.br/download/Documentos47.pdf. Acessed 22 August 2014.

Staden, R., Judge D.P., Bonfield, J.K. (2001). Sequence assembly and finishing methods. Methods Biochem Anal, 43: 303-322.

Souza, B.A., Carvalho, C.A., Alvez, M.O., Dias, C.S. (2009). Munduri: (Melipona asilvae) a abelha sestrosa. Grupo Insecta.UFRB: Cruz das Almas, Bahia.

Souza, C.B., Santos, D.M., Araújo, E.D. (2006). Nidificação de Mandaçaia (Melipona quadrifasciata quadrifasciata) em coqueirais da região da foz do rio São Francisco. Anais do XVI Congressso Brasileiro de Apicultura. Aracaju

Tamura, K., Dudley, J., Nei, M., Kumar, S. (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software versão 4.0. Molecular Biology and Evolution, 24: 1596-1599. doi:10.1093/molbev/msm092

Tavares, M.G., Pietrani, N.T., Durvale, M.C., Resende, H.C., Campos, L.A.O. (2013) Genetic divergence between Melipona quadrifasciata Lepeletier (Hymenoptera, Apidae) populations. Genetics and Molecular Biology, 36: 111-117.doi.10.1590/s1415-47572007000400027.

Waldschmidt, A.M., Marco-Júnior, P., Barros, E.G., Campos, L.A.O. (2002). Genetic Alalysis of Melipona quadrifasciata Lep. (Hymenoptera: Apidae, Meliponinae) With RAPD Markers. Brazilian Journal of Biology, 62: 923-928.

Waldschmidt, A.M., Barros, E.G., Campos, L.A.O. (2000). A molecular marker distinguishes the subspecies Melipona quadrifasciata quadrifasciata and Melipona quadrifasciata anthidioides (Hymenoptera, Apidae, Meliponinae). Genetics Molecular and Biology, 23: 609-611. doi:10.1590/S1415-47572000000300019.

Waldschmidt, A.M., Salomão, T.M.F., Barros, E.G., Campos, L.A.O. (1997). Methodology: Extration of genomic de DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae). Brazilian Journal of Genetics, 20: 421-423. doi:10.1590/S0100-84551997000300011.

Zietkiewicz, E., Ralfski, A., Labuda, D. (1994). Genome Frigerprinting by simple sequence (SSR) -Anchored Polymerase Chain Reaction Amplification. Genomics, 20: 176-183. doi:10.1006/geno.1994.1151.




DOI: http://dx.doi.org/10.13102/sociobiology.v63i2.946

Refbacks

  • There are currently no refbacks.


JCR Impact Factor 2018: 0.604