Molecular Characterization of Acute Bee Paralysis Virus (ABPV) and Black Queen Cell Virus (BQCV) in Honeybees (Apis mellifera L. (Hymenoptera: Apidae)) from the Campinas Region

Authors

  • Márcia Furlan Nogueira Embrapa Pantanal (CPAP), Corumbá-MS & Embrapa Environment (CNPMA), Jaguariúna-SP, Brazil
  • Camila Dantas Malossi Universidade Estadual Paulista “Julio de Mesquita Filho” (UNESP), Instituto de Biotecnologia (IBTEC), Botucatu - SP, Brazil
  • Maria Giulia Britto Frediani Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Meio Ambiente (CNPMA), Laboratório de Quarentena "Costa Lima"/ Laboratório de Entomologia e Fitopatologia, Jaguariúna, SP, Brazil
  • David Pereira Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Meio Ambiente (CNPMA), Laboratório de Quarentena "Costa Lima"/ Laboratório de Entomologia e Fitopatologia, Jaguariúna, SP, Brazil
  • Simone de Souza Prado Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Meio Ambiente (CNPMA), Laboratório de Quarentena "Costa Lima"/ Laboratório de Entomologia e Fitopatologia, Jaguariúna, SP, Brazil
  • João Pessoa Araujo Jr. Universidade Estadual Paulista “Julio de Mesquita Filho” (UNESP), Instituto de Biotecnologia (IBTEC), Botucatu, SP, Brazil
  • Cristiano Menezes Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Meio Ambiente (CNPMA), Laboratório de Quarentena "Costa Lima"/ Laboratório de Entomologia e Fitopatologia, Jaguariúna, SP, Brazil

DOI:

https://doi.org/10.13102/sociobiology.v71i2.10306

Keywords:

ABPV, BQCV, molecular detection, Sanger sequencing, bee health

Abstract

The occurrence of Colony Collapse Disorder (CCD) has prompted extensive research on the role of viruses, including Deformed Wing Virus (DWV), Acute Bee Paralysis Virus (ABPV), and Black Queen Cell Virus (BQCV), in honeybee health. This study investigated the presence and the genetic characteristics of DWV, ABPV, and BQCV in Apis mellifera colonies in a Brazilian apiary in Jaguariúna, SP, Brazil. A total of 11 apparently healthy colonies were sampled, and adult honeybees were submitted to a multiplex PCR. Results showed that six studied colonies tested positive for ABPV in at least one sampling, while one tested positive for BQCV. DWV was not detected, nor were co-infections observed in the sampled colonies. Although the infected colonies did not exhibit clinical signs of disease, the fluctuating presence of ABPV and BQCV suggests temporal variations in viral dynamics, possibly influenced by environmental and nutritional factors. The absence of DWV detection may be attributed to low infestation levels of the Varroa destructor mite in the sampled apiary. Phylogenetic analysis revealed close genetic relationships between the obtained ABPV and BQCV sequences and strains from South America. These findings contribute to the limited knowledge of viral epidemiology in Brazilian honeybee populations.

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References

Alonso-Prados, E., González-Porto, A.V., Bernal, J.L., Bernal, J., Martín-Hernández, R. & Higes, M. (2021). A case report of chronic stress in honey bee colonies induced by pathogens and acaricide residues. Pathogens, 10: 955.

Amiri, E., Strand, M.K., Rueppell, O. & Tarpy, D.R. (2017). Queen quality and the impact of honey bee diseases on queen health: potential for interactions between two major threats to colony health. Insects, 8: 48.

Beaurepaire, A., Piot, N., Doublet, V., Antunez, K., Campbell, E., Chantawannakul, P., Chejanovsky, N., Gajda, A., Heerman, M., Panziera, D., Smagghe, G., Yañez, O., de Miranda, J.R. & Dalmon, A. (2020). Diversity and global distribution of viruses of the western honey bee, Apis mellifera. Insects, 11: 239.

Benjeddou, M., Leat, N., Allsopp, M. & Davison, S. (2001). Detection of acute bee paralysis virus and black queen cell virus from honeybees by reverse transcriptase PCR. Applied and Environmental Microbiology, 67: 2384-2387.

de Miranda, J.R., Cordoni, G. & Budge, G. (2010). The Acute bee paralysis virus-Kashmir bee virus-Israeli acute paralysis virus complex. Journal of Invertebrate Pathology, 103 (Suppl 1): S30-S47.

Flores, J.M., Gámiz, V., Jiménez-Marín, Á., Flores-Cortés, A., Gil-Lebrero, S., Garrido, J.J. & Hernando, M.D. (2021). Impact of Varroa destructor and associated pathologies on the colony collapse disorder affecting honey bees. Research in Veterinary Science, 135: 85-95.

Guimarães-Cestaro, L., Martins, M.F., Martínez, L.C., Alves, M.L.T.M.F., Guidugli-Lazzarini, K.R., Nocelli, R.C.F., Malaspina, O., Serrão, J.E. & Teixeira, E.W. (2020). Occurrence of virus, microsporidia, and pesticide residues in three species of stingless bees (Apidae: Meliponini) in the field. The Science of Nature, 107: 16.

Hristov, P., Shumkova, R., Palova, N. & Neov, B. (2020). Factors associated with honey bee colony losses: a mini-review. Veterinary Sciences, 7: 166.

Jolivet, P. & Foley, J.W. (2015). Solutions for purifying nucleic acids by solid-phase reversible immobilization (SPRI). Ludmer Centre for Neuroinformatics and Mental Health. https://seaphages.org/media/forums/attachments/70027108-9277-4186-8413-29868d706794.pdf (accessed date: 12 July 2023).

Johnson, M., Zaretskaya, I., Raytselis, Y., Merezhuk, Y., McGinnis, S. & Madden, T.L. (2008). NCBI BLAST: a better web interface. Nucleic Acids Research, 36 (Web Server issue): W5-W9.

Martin, S.J. & Brettell, L.E. (2019) Deformed Wing Virus in honeybees and other insects. Annual Review of Virology, 6: 49-69.

Message, D., Ball, B.V., Allen, M. (1996). Ocorrência de viroses em abelhas no Brasil e na Argentina. In: Congresso Brasileiro de Apicultura, 11., 1996, Teresina. Anais. Teresina: Confederação Brasileira de Apicultura, 1996. p. 399.

Peixoto, C.M., França, S.O., Mercês, C.C., Correia-Oliveira, M.E. & de Carvalho, C.A.L. (2021). Occurrence of pathogenic viruses in africanized honey bees in Brazil. Journal of Apicultural Research, 63: 128-135.

Souza, F.S., Kevill, J.L., Correia-Oliveira, M.E., de Carvalho, C.A.L. & Martin, S.J. (2019). Occurrence of deformed wing virus variants in the stingless bee Melipona subnitida and honey bee Apis mellifera populations in Brazil. Journal of General Virology, 100: 289-294.

Teixeira, E.W., Chen, Y., Message, D., Pettis, J. & Evans, J.D. (2008). Virus infections in Brazilian honey bees. Journal of Invertebrate Pathology, 99: 117-119.

Teixeira, E.W., Ferreira, E.A., da Luz, C.F.P., Martins, M.F., Ramos, T.A. & Lourenço, A.P. (2020). European foulbrood in stingless bees (Apidae: Meliponini) in Brazil: old disease, renewed threat. Journal of Invertebrate Pathology, 172: 107357.

Ueira-Vieira, C., Almeida, L.O., de Almeida, F.C., Amaral, I.M.R., Brandeburgo, M.A.M. & Bonetti, A.M. (2015). Scientific note on the first molecular detection of the acute bee paralysis virus in Brazilian stingless bees. Apidologie, 46: 628-630.

Ullah, A., Gajger, I.T., Majoros, A., Dar, S.A., Khan, S., Kalimullah, Shah, A.H., Khabir, M.N., Hussain, R., Khan, H.U., Hameed, M. & Anjum, S.I. (2021). Viral impacts on honey bee populations: a review. Saudi Journal of Biological Sciences, 28: 523-530.

vanEngelsdorp, D., Evans, J.D., Saegerman, C., Mullin, C., Haubruge, E., Nguyen, B.K., Frazier, M., Frazier, J., Cox-Foster, D., Chen, Y., Underwood, R., Tarpy, D.R. & Pettis, J.S. (2009). Colony collapse disorder: a descriptive study. PLoS One, 4: e6481.

Ueno, T., Nakaoka ,T., Takeuchi, H. & Kubo, T. (2009). Differential gene expression in the hypopharyngeal glands of worker honeybees (Apis mellifera L.) associated with an age-dependent role change. Zoological Science, 26: 557-63.

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Published

2024-06-25

How to Cite

Nogueira, M. F., Malossi, C. D., Frediani, M. G. B., Pereira, D., Prado, S. de S., Araujo Jr., J. P., & Menezes, C. (2024). Molecular Characterization of Acute Bee Paralysis Virus (ABPV) and Black Queen Cell Virus (BQCV) in Honeybees (Apis mellifera L. (Hymenoptera: Apidae)) from the Campinas Region. Sociobiology, 71(2), e10306. https://doi.org/10.13102/sociobiology.v71i2.10306

Issue

Vol. 71 No. 2 (2024)

Section

Short Note

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Copyright (c) 2024 Márcia Furlan Nogueira, Camila Dantas Malossi, Maria Giulia Britto Frediani, David Pereira, Simone de Souza Prado, João Pessoa Araujo Jr., Cristiano Menezes

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