Population Diversity of Odontotermes formosanus (Shiraki) (Termitidae, Macrotermitinae) from Different Geographic Locations in Anhui Province, China

Authors

  • Yun Qiu Yang State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China
  • Long Jun Pu College of Life Science, Anhui Agricultural University, Hefei, China;Peking-Tsinghua Joint Center for Life Sciences, Peking University, Beijing, China
  • Qian Wang Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
  • Zhong Wang Institute of Termites Control of Bengbu, Bengbu, China
  • Zheng Ping Pang Institute of Termites Control of Wujin, Changzhou, China
  • Yan Hua Long College of Life Science, Anhui Agricultural University, Hefei, China

DOI:

https://doi.org/10.13102/sociobiology.v65i3.1146

Keywords:

genetic diversity, genetic variation, inter-simple sequence repeat, Odontotermes formosanus

Abstract

Genetic differentiation, genetic exchange, and influence of natural geographic barrier on the genetic structure of 20 geo-populations of Odontotermes formosanus sampled from different regions in Anhui province, China were detected using ISSR. Seventy-nine polymorphic loci were detected with nine ISSR primers, and the percentage of polymorphic bands was 87.78%. The average number of alleles per locus was 1.8778 ± 0.3294, and the effective number of alleles was 1.4741 ± 0.3438. The Nei′s gene diversity and Shannon information index were 0.2832 ± 0.1696 and 0.4307 ± 0.2274, respectively. All the populations were divided into two groups through UPGMA clustering analysis based on Nei’s genetic distance. One group comprised geo-populations A, C, and J, and the other group consisted of the remaining clusters. Mantel test results revealed no significant correlation between genetic similarity and geographical distance, as well as between elevation. High levels of genetic diversity, genetic mutation, and genetic differentiation were also detected among the geo-populations of O. formosanus. This study revealed the gene flow and possible migration paths of O. formosanus, which are necessary for continuous monitoring and prevention of this species.

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References

Aanen, D.K., Eggleton, P., Rouland–Lefevre, C., Guldberg– Froslev, T., Rosendahl, S. & Boomsma, J.J. (2002). The evolution of fungus-growing termites and their mutualistic fungal symbionts. Proceedings of the National Academy of Sciences of the United States of America 99: 14887-14892.

Aanen, D.K., Fine Licht, H.H., Debets, A.J.M., Kerstes, N.A.G., Hoekstra, R.F. & Boomsma J.J. (2009). High Symbiont Relatedness Stabilizes Mutualistic Cooperation in Fungus-Growing Termites. Science, 326(5956), 1103-1106. Doi: 10.1126/science.1173462

Appel, G.A., Hu, X.P., Zhou, J., Qin, Z., Zhu, H., Chang, X., Wang, Z., Liu, X. & Liu M. (2012). Observations of the Biology and Ecology of the Black-Winged Termite, Odontotermes formosanus Shiraki (Termitidae: Isoptera), in Camphor, Cinnamomum camphora (L.) (Lauraceae). Psyche, 5 pages, doi:10.1155/2012/123102.

Barbosa, N.C.C.P., Freitas, S., Morales, A.C. (2014). Distinct genetic structure in populations of Chrysoperla externa (Hagen) (Neuroptera, Chrysopidae) shown by genetic markers ISSR and COI gene. Revista Brasileira de Entomologia, 58(2), 203-211.

Beckenbach, A.T., Wei, Y.W. & Liu, H. (1993). Relationships in the Drosophila obscura Species Group, Inferred from Mitochondrial Cytochrome Oxidase II Sequences. Molecular Biology and Evolution, 10(3), 619−634. doi: 10.1093/Oxford journals.molbev.a040034

Black, H.I.J. & Okwakol, M.J.N. (1997). Agricultural intensification, soil biodiversity and agroecosystem function in the tropics: the role of termites. Applied Soil Ecology, 6(1), 37−53. doi:10.1016/S0929-1393(96)00153-9

Botstein, D., White, R.L., Skolnick, M. & Davis, R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3), 314−331.

Cao, P.J., Yao, Q.F., Ding, B.Y., Zeng, H.Y., Zhong, Y.X., Fu, C.X. & Jin, X.F. (2006). Genetic diversity of Sinojackia dolichocarpa (Styracaceae), a species endangered and endemic to China, detected by inter-simple sequence repeat (ISSR). Biochemical Systematics and Ecology, 34, 231−239. doi: 10. 1016/j.bse.2005.11.001

Duan, J.W., Liu, J., Ma, X.L., Zhang, Y., Wang, X.H. & Zhao, K. (2017). Isolation, Identification, and Expression of Microbial Cellulases from the Gut of Odontotermes formosanus. Journal of Microbiology and Biotechnology, 27(1), 122-129.

Dutta, S.R., Kar, P.K., Srivastava, A.K., Sinha, M.K., Shankar, J. & Ghosh, A.K. (2012). Identification of RAPD and SCAR markers associated with yield traits in the Indian tropical tasar silkworm Antheraea mylitta drury. Genetics and Molecular Biology, 35(4): 743–751. doi: 10.1590/S1415- 47572012005000059

Esselman, E.J., Jianqiang, L., Crawford, D.J., Windus, J.L. & Wolfe, A.D. (1999). Clonal diversity in the rare Calamagrostis porteri ssp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Molecular Ecology, 8(3): 443−451. doi: 10.1046/j.1365-294X.1999.00585.x

Ge, J.Y., Ju, P.C., Wang, X.B., Yan, A.P. & Jin, F. (2008). Current Situation of the Termites’ Damage on Garden Plant in Three Nanjing’s Parks. Journal of Jinling Institute of Technology, 24(4), 89−91. doi: 10.16515/j.cnki.32-1722/n.2008.04.016

Goodisman, M.A.D., Evans, T.A., Ewen, J.G. & Crozier, R.H. (2001). Microsatellite markers in the primitive térmite Mastotermes darwiniensis. Molecular Ecology Notes, 1: 250- 251. doi: 10.1046/j.1471-8278.2001.00094.x

Hall, T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/ NT. Nucleic Acids Symposium Series, 41, 95−98.

Harry, M., Roose, C.L., Garnier-Sillam, E. & Solignac, M. (2001). Microsatellite markers in soil-feeding termites (Cubitermes subarquatus, Isoptera, Termitidae, Termitinae). Molecular Ecology Notes, 1: 226-228. doi: 10.1046/j.1471- 8278.2001.00080.x

Hayashi, Y., Kitade, O. & Kojima, J.I.( 2002). Microsatellite loci in the Japanese subterranean termite, Reticulitermes speratus. Molecular Ecology Notes, 2: 518-520. doi: 10.1046/ j.1471-8278.2002.00299.x

Hu, J., Zhong, J.H., & Guo, M.F. (2007). Alate Dispersal Distances of the Black-Winged Subterranean Termite Odontotermes formosanus (Isoptera: Termitidae) in Southern China. Sociobiology, 50: 1−8.

Hu, B.J., Xu, L.N., Zhou, Z.Y., Hu, F., Luan, F.G., Chen, X., Li, Z.Z. (2015). Molecularr tracing of white muscardine in Asian corn borer using inter-simple sequence repeat (ISSR) analysis. Genetics and Molecular Research, 14(4), 18720-18730. doi: 0.4238/2015.December.28.21

Huang, F. S., Zhu, S. M., Ping, Z. M., He, X. S., Li., G. X. & Gao, D. R. (2000). Fauna Sinica: Insecta, vol. 17: Isoptera. Science Press, Beijing, China.

Huang, Q.Y., Lei, C.L. & Xue D. (2006). Field evaluation of a fipronil bait against subterranean termite Odonototermes formosanus (Isoptera: Termitidae). Journal of Economic Entomology, 99: 455-461.

Huang, Q.Y., Wang, W.P., Mo, R.Y. & Lei, C.L. (2008). Studies on feeding and trophallaxis in the subterranean termite Odontotermes formosanus using rubidium chloride. Entomologia Experimentalis et Applicata, 129: 120-125. doi: 10.1111/j.1570-7458.2008.00764.x

Huang, Q., Li, G, Husseneder, C. & Lei, C. (2013). Genetic Analysis of Population Structure and Reproductive Mode of the Termite Reticulitermes chinensis Snyder. PLOS ONE, 8(7): e69070. doi: 10.1371/journal.pone.0069070

Husseneder, C., Simms, D.M., Delatte, J.R., Wang, C., Grace, J.K. & Vargo, E.L. (2012). Genetic diversity and colony breeding structure in native and introduced ranges of the formosan subterranean termite, Coptotermes formosanus. Biological Invasions, 14: 419-437. doi: 10.1007/s10530-011-0087-7

Husseneder, C., Garner, S.P., Huang, Q., Booth, W. & Vargo, E.L. (2013). Characterization of microsatellites for population genetic analyses of the fungus-growing termite Odontotermes formosanus (Isoptera: Termitidae). Environmental Entomology, 42(5):1092-1099. doi: 10.1603/EN13059

Kaib, M., Hacker, M., Over, I., Hardt, C., Epplen, J.T., Bagine, R.K.N. & Brandl, R. (2000). Microsatellite loci in Macrotermes michaelseni (Isoptera: Termitidae). Molecular Ecology, 9: 502-504. doi: 10.1046/j.1365-294x.2000.00871-9.x

Li, J.H., Simon, G., Su, Z.H. & Pang, H. (2009). Genetic diversity of populations of Harmonia axyridis (Coleoptera: Coccinellidae) from USA and China revealed by ISSR-PCR. Journal of Environmental Entomology, 31(4): 311−319. doi: 10.3969/j.issn.1674-0858.2009.04.004

Liu, H. & Beckenbach, A.T. (1992). Evolution of the mitochondrial cytochrome oxidase II gene among 10 orders of insects. Molecular Phylogenetics And Evolution, 1: 41−52. doi: 10.1016/1055-7903(92)90034-E

Lo, N., Kitade, O., Miura, T., Constantino, R. & Matsumoto, T. (2004). Molecular phylogeny of the Rhinotermitidae. Insectes Sociaux, 51: 365−371. doi: 10.1007/s00040-004-0759-8

Long, Y.H., Xiang, H., Xie, L., Yan, X., Fan, M.Z. & Wang, Q. (2009). Intra- and Interspecific Analysis of Genetic Diversity and Phylogeny of Termites (Isoptera) in East China Detected by ISSR and COII Markers. Sociobiology, 53: 411−430.

Lu, N.H., Zheng, W.M., Wang, J.F., Zhan, G.M., Huang, L.L. & Kang, Z.S. (2009). SSR analysis of population genetic diversity of Puccinia striiformis f.sp. tritici in Longnan region of Gansu, China. Scientia Agricultura Sinica, 42: 2763−2770. doi: 10.3864/j.issn.0578-1752.2009.08.015

Menzies, J.G., Bakkeren, G., Matheson, F., Procunier, J.D. & Woods, S. (2003). Use of inter-simple sequence repeats and amplified fragment length polymorphisms to analyze genetic relationships among small grain-infecting species of Ustilago. Phytopathology, 93(2): 167−175. doi: 10.1094/PHYTO.2003.93.2.167.

Mühlemann, R.A., Bignell, D.E., Veivers, P.C., Leuthold, R.H. & Slaytor, M. (1995). Morphological, microbiological and biochemical studies of the gut flora in the fungusgrowing termite Macrotermes subhyalinus. Journal of Insect Physiology, 41: 929−940. doi: 10.1016/0022-1910(95)00062-Y

Nagaoka, T. & Ogihara, Y. (1997). Applicability of intersimple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theoretical and Applied Genetics, 94(5): 597−602. doi: 10.1007/s001220050456

Naik, A., Prajapat, P., Krishnamurthy, R., Pathak, J.M. (2017). Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers. 3 Biotech, 7: 70. doi: 10.1007/s13205-017-0667-z

Nybom, H. (2004). Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology, 13(5): 1143−1155. doi: 10.1111/j.1365-294X.2004.02141.x

Osiemo, Z.B., Marten, A., Kaib, M., Gitonga, L.M., Boga, H.I. & Brandl R. (2010). Open relationships in the castles of clay: high diversity and low host specificity of Termitomyces fungi associated with fungus-growing termites in Africa. Insectes Sociaux, 57, 351-363. doi: 10.1007/s00040-010-0092-3

Qian, W., Ge, S. & Hong, D.Y. (2001). Genetic variation within and among populations of a wild rice Oryza granulate from China detected by RAPD and ISSR markers. Theoretical and Applied Genetics, 102: 440−449. doi: 10.1007/s001220051665

Sperling, Felix A.H., Anderson, G.S. & Hickey, D.A. (1994). A DNA-Based Approach to the Identification of Insect Species Used for Postmorten Interval Estimation. Journal of Forensic Science, 39: 418−427. doi: 10.1520/JFS13613J

Su., L.J., Yang, L.L., Huang, S., Su, X.Q., Li, Y., Wang, F.Q., Wang, E.T., Kang, N., Xu, J., & Song, A.D. (2016). Comparative Gut Microbiomes of Four Species Representing the Higher and the Lower Termites. Journal of Insect Science, 16(1): 97; 1–9. doi: 10.1093/jisesa/iew081

Tanya, P., Taeprayoon, P., Hadkam, Y. & Srinives, P. (2011). Genetic Diversity Among Jatropha and Jatropha-Related Species Based on ISSR Markers. Plant Molecular Biology Reporter, 29, 252−264. doi: 10.1007/s11105-010-0220-2

Thompson, G.J., Lenz, M. & Crozier, R.H. (2000). Microsatellites in the subterranean, mound-building térmite Coptotermes lacteus (Isoptera : Rhinotermitidae). Molecular Ecology, 9: 1932-1934. doi: 10.1046/j.1365-294x.2000.01080-9.x

Tsumura, Y., Ohba, K. & Strauss, S.H. (1996). Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica). Theoretical and Applied Genetics, 92(1), 40−45. doi: 10.1007/BF00222949

Vargo, E. L. (2000). Polymorphism at trinucleotide microsatellite loci in the subterranean termite Reticulitermes flavipes. Molecular Ecology, 9: 817-820. Vargo, E.L. & Henderson, G. (2000). Identification of polymorphic microsatellite loci in the Formosan subterranean termite Coptotermes formosanus Shiraki. Molecular Ecology, 9: 1935-1938. doi: 10.1046/j.1365-294x.2000.0090111935.x

Vargo, E. L. (2003a). Genetic structure of Reticulitermes flavipes and R. virginicus (Isoptera: Rhinotermitidae) colonies in an urban habitat and tracking of colonies following treatment with hexaflumuron bait. Environmental Entomology, 32: 1271–1282. doi: 10.1603/0046-225X-32.5.1271

Vargo, E. L. (2003b). Hierarchical analysis of colony and population genetic structure of the eastern subterranean termite, Reticulitermes flavipes, using two classes of molecular genetic markers. Evolution, 57: 2805-2818.

Vargo, E.L., Husseneder, C. & Grace, J.K. (2003). Colony and population genetic structure of the Formosan subterranean termite, Coptotermes formosanus, in Japan. Molecular Ecology, 12: 2599-2608. doi: 10.1046/j.1365-294X.2003.01938.x

Wallman, J.F. & Donnellan, S.C. (2001). The utility of mitochondrial DNA sequences for the identification of forensically important blowflies (Diptera:Calliphoridae) in southeastern Australia. Forensic Science International, 120, 60−67. doi: 10.1126/science.3018931

Yang, J., Tian, L., Xu, B., Xie, W., Wang, S., Zhang, Y., Wang, X. & Wu, Q. (2015). Insight into the Migration Routes of Plutella xylostella in China Using mtCOI and ISSR Markers. PLoS ONE, 10(6), e0130905. doi: 10.1371/journal.pone.0130905

Yashiro, T., Matsuura, K. & Tanaka, C. (2011). Genetic diversity of termite-egg mimicking fungi “termite balls” within the nests of termites. Insectes Sociaux, 58: 57-64. doi: 10.1007/s00040-010-0116-z

Yeh, F.C., Yang, R.C., Boyle, T., Timothy, B.J., Ye, Z.H. & Mao, J.X. (1997). POPGENE, the user-friendly shareware for population genetic analysis. Molecular biology and biotechnology centre, university of Alberta, Canada.

Zhao, G.H., Li, J., Zou, F.C., Mo, X.H., Yuan, Z.G., Lin, R.Q., Weng, Y.B. & Zhu, X.Q. (2009). ISSR, an effective molecular approach for studying genetic variability among Schistosoma japonicum isolates from different provinces in mainland China. Infection Genetics and Evolution, 9(5), 903−907. doi: 10.1016/j.meegid.2009.06.006

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Published

2018-10-02

How to Cite

Yang, Y. Q., Pu, L. J., Wang, Q., Wang, Z., Pang, Z. P., & Long, Y. H. (2018). Population Diversity of Odontotermes formosanus (Shiraki) (Termitidae, Macrotermitinae) from Different Geographic Locations in Anhui Province, China. Sociobiology, 65(3), 497–505. https://doi.org/10.13102/sociobiology.v65i3.1146

Issue

Vol. 65 No. 3 (2018)

Section

Research Article - Termites

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