First Characterization of Sphingomyeline Phosphodiesterase Expression in the Bumblebee, Bombus lantschouensis


  • lei Han Eastern Bee Research Institute, Yunnan Agricultural University;Institute of Apicultural Research, Chinese Academy of Agricultural Sciences
  • Shaoyu He Eastern Bee Research Institute, Yunnan Agricultural University
  • Jie Dong Institute of Apicultural Research, Chinese Academy of Agricultural Sciences
  • Ye Wang Institute of Apicultural Research, Chinese Academy of Agricultural Sciences
  • Jiaxing Huang Institute of Apicultural Research, Chinese Academy of Agricultural Sciences
  • Jie Wu Institute of Apicultural Research, Chinese Academy of Agricultural Sciences



Bombus lantschouensis, SMPD gene, gene clone, prokaryotic expression,


  The bumblebee (Bombus lantschouensis Vogt) is an important pollinator of wild plants. Sphingomyelin phosphodiesterase (SMPD) is a hydrolase that plays a major role in sphingolipid metabolism reactions. We report the preparation and characterization of a polyclonal antibody for bumblebee SMPD. We then use the polyclonal antiserum to detect the SMPD protein at different development stages and in different tissues. Our results showed that a 1228bp fragment homologous with the B. terrestris SMPD gene was successfully amplified. The molecular weight of the fusion protein was about 70 kDa by SDS-PAGE. An effective polyclonal antibody against SMPD was also obtained from mice and found to have a higher specificity for bumblebee SMPD. Western blotting detection showed that SMPD was expressed at a high level in queen ovaries, although expression was lower in the midgut and venom gland. SMPD expression decreased from the egg stage until the pdd stage. We interpret our results as showing that the development of an effective polyclonal antiserum for the SMPD protein of a bumblebee, which provides a tool for exploring the function of the SMPD gene. In addition, the work has confirmed that SMPD should be considered as an important enzyme during bumblebee egg and larval stages.


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How to Cite

Han, lei, He, S., Dong, J., Wang, Y., Huang, J., & Wu, J. (2017). First Characterization of Sphingomyeline Phosphodiesterase Expression in the Bumblebee, Bombus lantschouensis. Sociobiology, 64(1), 85–91.



Research Article - Bees