Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites

Authors

  • Yu-Lei Dang Northwest University
  • Hong-Gui Zhang Northwest University
  • Yu-Feng Meng Northwest University
  • Min Zhang Northwest University
  • Sha Zhao Northwest University
  • Ping You Shaanxi Normal University
  • Xiao-Hong Su Northwest University
  • Lian-Xi Xing Northwest University

DOI:

https://doi.org/10.13102/sociobiology.v64i3.1692

Keywords:

Subterranean termites, Reticulitermes, Microsatellite loci,  Genetic diversity

Abstract

We isolated 15 and 18 highly polymorphic genomic microsatellite markers from two subterranean termites, Reticulitermes aculabialis and R. labralis, respectively. A total of 53 alleles were detected in 15 microsatellite loci of R. aculabialis, and the alleles were 3.533±1.302 (mean±SD), while the corresponding data of R. labralis were 115 detected alleles in 18 microsatellite loci with 6.389±1.754 alleles. The observed and expected heterozygosity was 0.496±0.236 and 0.564±0.125 in R. aculabialis, and 0.368±0.263 and 0.702±0.115 in R. labralis, respectively. Seven loci were highly polymorphic (PIC>0.5) in R. aculabialis, and 15 loci were highly polymorphic (PIC>0.5) in R. labralis. All loci showed Hardy–Weinberg equilibrium. These polymorphic markers provide useful tools for population genetic and breeding system studies of subterranean termites.

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Author Biographies

Yu-Lei Dang, Northwest University

College of Life Sciences

Hong-Gui Zhang, Northwest University

College of Life Sciences

Lian-Xi Xing, Northwest University

College of Life Sciences

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Published

2017-10-17

How to Cite

Dang, Y.-L., Zhang, H.-G., Meng, Y.-F., Zhang, M., Zhao, S., You, P., Su, X.-H., & Xing, L.-X. (2017). Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites. Sociobiology, 64(3), 352–355. https://doi.org/10.13102/sociobiology.v64i3.1692

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Short Note