Assessing the Utility of a PCR Diagnostics Marker for the Identification of Africanized Honey Bee, Apis mellifera L., (Hymenoptera: Apidae) in the United States.

Authors

  • Allen Szalanski University of Arkansas
  • Amber Tripodi University of Arkansas

DOI:

https://doi.org/10.13102/sociobiology.v61i2.234-236

Keywords:

Apis mellifera, Africanized honey bee, mtDNA, molecular diagnostics

Abstract

An assessment of a molecular diagnostic technique for distinguishing Africanized honey bees from European honey bees in the United States was conducted. Results from multiplex PCR diagnostics of a mitochondrial DNA cyt-b marker corresponded with results based on COI-COII sequencing analysis, but differed from morphometric analysis results. We suggest utilizing both multiplex PCR and morphometric methods for Africanized honey bee diagnostics in the United States, when possible.

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Author Biography

Allen Szalanski, University of Arkansas

Professor

References

Anonymous (2011). Map of the spread of Africanized honey bee by year. http://www.ars.usda.gov/research/docs.htm?docid=11059&page=6.

Berry, J. (2011). African honey bees in Georgia. Bee Culture 139(2): 53-55.

Crozier, Y. C., S. Koulianos, & R. H. Crozier (1991). An improved test for Africanized honey bee mitochondrial DNA. Experientia 47(9): 968-969. DOI: https://doi.org/10.1007/BF01929894

Franck, P., L. Garnery, A. Loiseau, B. P. Oldroyd, H. R. Hepburn, M. Solignac, & J-M. Cornuet (2001). Genetic diversity of the honey bee in Africa: microsatellite and mitochondrial data. Hered. 86(4): 420-430. DOI: https://doi.org/10.1046/j.1365-2540.2001.00842.x

Francoy T. M., D. Wittmann, M. Drauschke, S. Muller, V. Steinhage, M. A. Bezerra Laure, D. De Jong, & L. S. Gonçalves. (2008). Identification of Africanized honey bees through wing morphometrics: two fast and efficient procedures. Apidologie 39(5): 488-494. DOI: https://doi.org/10.1051/apido:2008028

Magnus, R. M., & A. L. Szalanski. (2010). Genetic evidence of honey bees belonging to the Middle East lineage in the United States. Sociobiol. 55(1B): 285-296.

Meixner M. D., M. A. Pinto, M. Bouga, P. Kryger, E. Ivanova, & S. Fuchs. (2013). Standard methods for characterising subspecies and ecotypes of Apis mellifera (Paper 52.04.05). COLOSS BEEBOOK, Vol. 1 Special Issue, J. Apicul. Res. 52(4):1-27. DOI: https://doi.org/10.3896/IBRA.1.52.4.05

Pinto, M. A., J. S. Johnson, W. L. Rubink, R. N. Coulson, J. C. Patton, & W. S. Sheppard. (2003). Identification of Africanized honey bee (Hymenoptera: Apidae) mitochondrial DNA: validation of a rapid polymerase chain reaction-based assay. Ann. Entomol. Soc. Amer. 96(5): 679-684. DOI: https://doi.org/10.1603/0013-8746(2003)096[0679:IOAHBH]2.0.CO;2

Rinderer, T. E., S. M. Buco, W. L. Rubink, H. V. Daly, J. A. Stelzer, R. M. Riggio, & F. C. Baptista. (1993). Morphometric identification of Africanized and European honey bees using large reference populations. Apidologie 24(6): 569-585. DOI: https://doi.org/10.1051/apido:19930605

Ruttner, F. (1987). Biogeography and taxonomy of honeybees. Springer-Verlag, Berlin. 284 pp. DOI: https://doi.org/10.1007/978-3-642-72649-1

Sheppard, W. S., & D. R. Smith. (2000). Identification of African-derived bees in the Americas: a survey of methods. Ann. Entomol. Soc. Amer. 93(2): 159-176. DOI: https://doi.org/10.1603/0013-8746(2000)093[0159:IOADBI]2.0.CO;2

Sugden, D. A., & K. R. Williams. (1990). October 15: the day the bee arrived. Gleanings in Bee Culture 119: 18-21.

Sylvester, H. A., & T. E. Rinderer. (1987). Fast Africanized bee identification system (FABIS) manual. Amer. Bee J. 127(7): 511-516.

Szalanski, A. L., & J. A. McKern. (2007). Multiplex PCR-RFLP diagnostics of the Africanized honey bee (Hymenoptera: Apidae). Sociobiol. 50(3): 939-945.

Szalanski, A. L., & R. M. Magnus. (2010). Mitochondrial DNA characterization of Africanized honey bee (Apis mellifera L.) populations from the USA. J. Apicul. Res. 49(2): 177-185. DOI: https://doi.org/10.3896/IBRA.1.49.2.06

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Published

2014-07-01

How to Cite

Szalanski, A., & Tripodi, A. (2014). Assessing the Utility of a PCR Diagnostics Marker for the Identification of Africanized Honey Bee, Apis mellifera L., (Hymenoptera: Apidae) in the United States. Sociobiology, 61(2), 234–236. https://doi.org/10.13102/sociobiology.v61i2.234-236

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Short Note