Characterizing the Mitogenome of the Endemic Bumblebee Subspecies from the Canary Islands for Conservation Purposes

Authors

DOI:

https://doi.org/10.13102/sociobiology.v68i3.5910

Keywords:

Bumblebee, mitogenome, conservation, endemic subspecies, Bombus terrestris canariensis, Canary Islands

Abstract

The taxonomic status of Bombus terrestris subspecies is complex and has deep implications in the management of commercial bumblebees for crop pollination as well as in the establishment of appropriate conservation plans. Herein, the complete mitogenome of the endemic Canary Islands subspecies Bombus terrestris canariensis is newly sequenced and compared with available mitochondrial sequences in order to shed light into its taxonomic status. The mitochondrial genome was 17,300 bp in length and contained 37 genes, including 13 protein-coding genes (PCGs), two rRNAs, and 22 tRNAs and a partial sequence of the AT rich control region. The phylogenetic analysis of PCGs of the mitogenome was congruent with its subspecific status and a close relationship with the North African subspecies africanus as previously suggested. The sequencing of the mitogenome of B. t. canariensis provides useful genetic information to study the conservation genetics and genetic diversity of these island bumblebee populations.

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Author Biographies

Carlos Ruiz, Dpto. Biología Animal, Edafología y Geología, Facultad de Ciencias, Universidad de La Laguna. La Laguna, 38206 Tenerife

Assitant Professor

Diego Cejas, Université de Mons, Laboratoire de Zoologie, Place du Parc 23, 7000 Mons

PhD student

Irene Muñoz, Dpto. de Zoología y Antropología Física, Facultad de Veterinaria, Universidad de Murcia. Espinardo, 30100 Murcia

Postdoctoral researcher

Pilar De la Rua, University of Murcia

Full Professor

References

Arbetman, M.P., Meeus, I., Morales, C.L., Aizen, M.A. & Smagghe, G. (2013). Alien parasite hitchhikes to Patagonia on invasive bumblebee. Biological Invasions, 15: 489 - 494. doi: 10.1007/s10530-012-0311-0

Bernt, M., Donath, A., Jühling, F., Externbrink, F., Florentz, C., Fritzsch, G., ... & Stadler, P. F. (2013). MITOS: improved de novo metazoan mitochondrial genome annotation. Molecular Phylogenetics and Evolution, 69: 313-319. doi: 10.1016/j. ympev.2012.08.023

Bertsch, A. (2010). A phylogenetic framework for the bumblebee species of the subgenus Bombus sensu stricto based on mitochondrial DNA markers, with a short description of the neglected taxon B. minshanicola Bischoff, 1936 n. status. (Hymenoptera: Apidae: Bombus). Beiträge Zur Entomologie, 60: 471-487. doi: 10.21248/contrib.entomol.60.2.471-487

Bartomeus, I., Molina, F.P., Hidalgo-Galiana, A. & Ortego, J. (2020). Safeguarding the genetic integrity of native pollinators requires stronger regulations on commercial lines. Ecological Solutions and Evidence, 1(1), e12012

Cejas, D., Ornosa, C., Muñoz, I. & De la Rúa, P. (2018). Searching for molecular markers to differentiate Bombus terrestris (Linnaeus) subspecies in the Iberian Peninsula. Sociobiology, 65: 558-565. doi: 10.13102/sociobiology.v65i4.3442

Cejas, D., López-López, A., Muñoz, I., Ornosa, C. & De la Rúa, P. (2020). Unveiling introgression in bumblebee (Bombus terrestris) populations through mitogenome-based markers. Animal Genetics, 51: 70 -77. doi: 10.1111/age.12874

De Meulemeester, T. (2012). Approche intégrative dans la systématique de taxons complexes: bourdons et abeilles fossiles. Disertation, Université de Mons Du, Q., Bi, G,. Zhao, E., Yang, J., Zhang, Z. & Liu, G. (2016). Complete mitochondrial genome of Bombus terrestris (Hymenoptera: Apidae). Mitochondrial DNA Part A, 27: 4455-4456. doi: 10.3109/19401736.2015.1089568

Erlandsson, S. (1979). Bombus canariensis Pérez, 1895 n. stat and B. maderensis n. sp. from the Macaronesian Islands. Insect Systematics and Evolution, 10: 187-192

Estoup, A., Solignac, M., Cornuet, J.M., Goudet, J. & Scholl, A. (1996). Genetic differentiation of continental and island populations of Bombus terrestris (Hymenoptera: Apidae) in Europe. Molecular Ecology, 5: 19-31

Goka, K., Okabe, K., Yoneda, M. & Niwa, S. (2001). Bumblebee commercialization will cause worldwide migration of parasitic mites. Molecular Ecology, 10: 2095-2099. doi: 10.1046/j.0962-1083.2001.01323.x

Ings, T.C., Ward, N.L.L. & Chittka, L. (2006). Can commercially imported bumble bees out-compete their native conspecifics? Journal of Applied Ecology, 43: 940-948. doi: 10.1111/j.1365-2664.2006.01199.x

Inoue, M.N., Yokoyama, J. & Washitani, I. (2008). Displacement of Japanese native bumblebees by the recently introduced Bombus terrestris (L.) (Hymenoptera: Apidae). Journal of Insect Conservation, 12: 135-146. doi: 10.1007/s10841-007-9071-z

Kondo, N.I., Yamanaka, D., Kanbe, Y., Kunitake, Y.K., Yoneda, M., Tsuchida, K. & Goka, K. (2009). Reproductive disturbance of Japanese bumblebees by the introduced European bumblebee Bombus terrestris. Naturwissenschaften, 96: 467-475. doi: 10.1007/s00114-008-0495-4

Lecocq, T., Brasero, N., De Meulemeester, T., Michez, D., Dellicour, S., Lhomme, P., et al (2015). An integrative taxonomic approach to assess the status of Corsican bumblebees: implications for conservation. Animal Conservation, 18: 236-248. doi: 10.1111/acv.12164

Lecocq, T., Coppée, A., Michez, D., Brasero, N., Rasplus, J.Y., Valterová, I. & Rasmont, P. (2016). The alien’s identity: consequences of taxonomic status for the international bumblebee trade regulations. Biological Conservation, 195: 169-176. doi: 10.1016/j.biocon.2016.01.004

Lin, G., Jiang, K., Su, T., He, B., Huang, Z. & Zhao, F. (2019). Complete mitochondrial genome of Bombus waltoni (Hymenoptera: Apidae). Mitochondrial DNA Part B, 4: 1484-1485. doi: 10.1080/23802359.2019.1601528

Matsumura, C., Yokoyama, J. & Washitani I. (2004). Invasion status and potential ecological impacts of an invasive alien bumblebee, Bombus terrestris L. (Hymenoptera: Apidae) naturalized in Southern Hokkaido, Japan Global Environmental Research, 8: 51-66.

Morales, C. L., Arbetman, M. P., Cameron, S. A., & Aizen, M. A. (2013). Rapid ecological replacement of a native bumble bee by invasive species. Frontiers in Ecology and the Environment, 11: 529-534. doi: 10.1890/120321

Nagamitsu, T., Yamagishi, H., Kenta, T., Inari, N. & Kato, E. (2010). Competitive effects of the exotic Bombus terrestris on native bumble bees revealed by a field removal experiment. Population Ecology, 52: 123-136. doi: 10.1007/s10144-009-0151-7

Nishimoto, M., Umezawa, M., Okuyama, H., Kumano, N., Nomura, T. & Takahashi, J.I. (2018). The complete mitochondrial genome of the bumblebee, Bombus hypocrite sapporensis (Insecta: Hymenoptera: Apidae) from Hokkaido Island, Japan. Mitochondrial DNA Part B, 3: 354-356. doi: 10.1080/23802359.2018.1450673

Rasmont, P., Coppée, A., Michez, D. & De Meulemeester, T. (2008). An overview of the Bombus terrestris (L. 1758) subspecies (Hymenoptera: Apidae). Annales de la Société Entomologique de France, 44: 243-250. doi: 10.1080/00379271.2008.10697559

Ronquist, F. & Huelsenbeck, J.P. (2003). MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19: 1572-1574. doi: 10.1093/bioinformatics/btg180

Schmid-Hempel, R., Eckhardt, M., Goulson, D., Heinzmann, D., Lange, C., Plischuk, S., et al (2014) The invasion of southern South America by imported bumblebees and associated parasites. Journal of Animal Ecology, 83: 823-837. doi: 10.1111/1365-2656.12185

Seabra, S.G., Silva, S.E., Nunes, V.L., Sousa, V.C., Martins, J., Marabuto, E. et al (2019). Genomic signatures of introgression between commercial and native bumblebees, Bombus terrestris, in western Iberian Peninsula - Implications for conservation and trade regulation. Evolutionary Applications, 12: 679-691. doi: 10.1111/eva.12732

Tsuchida, K., Yamaguchi, A., Kanbe, Y., & Goka, K. (2019). Reproductive interference in an introduced bumblebee: Polyandry may mitigate negative reproductive impact. Insects, 10: 59. doi: 10.3390/insects10020059

van den Eijnde, J. & de Ruijter, A. (2000). Bumblebees from the Canary Islands: mating experiments with Bombus terrestris L. from the Netherlands. Proceedings of the Section Experimental and Applied Entomology, 11: 159-161.

Velthuis, H.H. & van Doorn, A. (2004). The breeding, commercialization and economic value of bumblebees. In: Freitas BM, Pereira JOP (Eds) Solitary bees: Conservation, Rearing and Management in Pollination. Universitaria, Fortaleza, pp 135-149.

Velthuis, H.H. & Van Doorn, A. (2006). A century of advances in bumblebee domestication and the economic and environmental aspects of its commercialization for pollination. Apidologie, 37: 421-451. doi: 10.1051/apido:2006019

Widmer, A., Schmid-Hempel, P., Estoup, A. & Scholl, A. (1998). Population genetic structure and colonization history of Bombus terrestris s.l. (Hymenoptera: Apidae) from the Canary Islands and Madeira. Heredity, 81: 563-572.

Yoon, H.J., Kim, S.Y., Lee, K.Y., Lee, S.B., Park, I.G. & Kim, I.S. (2009). Interspecific hybridization of the bumblebees Bombus ignitus and B. terrestris. International Journal of Industrial Entomology, 18: 41-48

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Published

2021-08-14

How to Cite

Ruiz, C., Cejas, D., Muñoz, I., & De la Rua, P. (2021). Characterizing the Mitogenome of the Endemic Bumblebee Subspecies from the Canary Islands for Conservation Purposes. Sociobiology, 68(3), e5910. https://doi.org/10.13102/sociobiology.v68i3.5910

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Short Note