Litter Surface Temperature: A Driving Factor Affecting Foraging Activity in Dinoponera lucida (Hymenoptera: Formicidae)




optimal foraging, poneromorph ants, litter surface, microclimate, diet


Dinoponera lucida is a poneromorph ant endemic to the Atlantic Forest of Brazil. The species is classified as endangered in Brazil’s Red List due to its peculiar reproductive biology and high habitat fragmentation. Herein, we characterize D. lucida foraging activity and response to litter surface temperature in a lowland forest remnant in south-eastern Brazil. The mean flow of workers at nest openings was 3.8 ± 0.6 per hour, mean foraging trip was 14.2 ± 2.2 min, and mean foraging distance was 3.8 ± 0.4 m. The time spent per foraging trip and litter surface temperature were positively correlated. Flow of workers at nest openings was higher with mean temperature of litter surface between 21.0 and 27.0 °C. Our results show that D. lucida has a diurnal foraging activity related to habitat temperature. Our data contribute to the knowledge about the ecology of D. lucida and support the hypothesis of optimal food foraging regulated by habitat temperature. In addition, the better understanding of D. lucida activity patterns can assist on conservation planning of this endangered and endemic ant.


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Almeida, F.S. & Queiroz, J.M. (2015). Formigas poneromorfas como engenheiras de ecossistemas: Impactos sobre a biologia, estrutura e fertilidade dos solos. In: As formigas poneromorfas do Brasil (eds. Delabie, J.H.C., Feitosa, R.M., Serrão, J.E., Mariano, C.S.F. & Majer, J.D.). Editus, Ilhéus, pp. 439–449. DOI:

Altmann, J. (1974). Observational study of behavior: Sampling methods. Behaviour, 49, 227–267. DOI:

Alvares, C.A., Stape, J.L., Sentelhas, P.C., De Moraes Gonçalves, J.L. & Sparovek, G. (2014). Köppen’s climate classification map for Brazil. Meteorologische Zeitschrift, 22, 711–728. DOI:

Araujo, A., Medeiros, J.C., Azevedo, D.L.O., Medeiros, I.A., Neto, W.A.S. & Garcia, D. (2015). Poneromorfas sem rainhas - Dinoponera: Aspectos ecológico-comportamentais. In: As formigas poneromorfas do Brasil (eds. Delabie, J.H.C., Feitosa, R.M., Serrão, J.E., Mariano, C.S.F. & Majer, J.D.). Editus, Ilhéus, pp. 237–246. DOI:

Araújo, A. & Rodrigues, Z. (2006). Foraging behavior of the queenless ant Dinoponera quadriceps Santschi (Hymenoptera: Formicidae). Neotropical Entomology, 35, 159–164. DOI:

Azevedo, D.L.O., Medeiros, J.C. & Araújo, A. (2014). Adjustments in the time, distance and direction of foraging in Dinoponera quadriceps workers. Journal of Insect Behavior, 27, 177–191. DOI:

Bernstein, R.A. (1979). Schedules of foraging activity in species of ants. Journal Animal Ecology, 48, 921–930. DOI:

Boehnke, D., Gebhardt, R., Petney, T. & Norra, S. (2017). On the complexity of measuring forests microclimate and interpreting its relevance in habitat ecology: The example of Ixodes ricinus ticks. Parasites and Vectors, 10, 1–14. DOI:

Campiolo, S., Rosário, N.A., Strenzel, G.M.R., Feitosa, R. & Delabie, J.H.C. (2015). Conservação de poneromorfas no Brasil. In: As formigas poneromorfas do Brasil (eds. Delabie, J.H.C., Feitosa, R.M., Serrão, J.E., Mariano, C.S.F. & Majer, J.D.). Editus, Ilhéus, pp. 447–462. DOI:

Carvalho, C.B., Santos, R.A., Rocha, S.M., Caldas, F.L.S., Freitas, E.B., Silva, B.D., et al. (2012). Gymnodactylus geckoides (Naked-toed gecko) predation. Herpetological Bulletin, 121, 40–43.

Chown, S.L., Sørensen, J.G. & Terblanche, J.S. (2011). Water loss in insects: An environmental change perspective. Journal of Insect Physiology, 57, 1070–1084. DOI:

Colinet, H., Sinclair, B.J., Vernon, P. & Renault, D. (2015). Insects in fluctuating thermal environments. Annual Review of Entomology, 60, 123–140. DOI:

Emlen, J.M. (1966). The role of time and energy in food preference. The American Naturalist, 100, 611–617. DOI:

Escárraga, M.E., Lattke, J.E. & Azevedo, C.O. (2017). Discovery of the Dinoponera lucida male (Hymenoptera, Formicidae), a threatened giant ant from the Atlantic rain forest. Zootaxa, 4347, 128–136. DOI:

Fourcassié, V. & Oliveira, P.S. (2002). Foraging ecology of the giant Amazonian ant Dinoponera gigantea (Hymenoptera, Formicidae, Ponerinae): Activity schedule, diet and spatial foraging patterns. Journal of Natural History, 36, 2211–2227. DOI:

Fundação SOS Mata Atlântica & Instituto Nacional de Pesquisas Espaciais. (2019). Atlas dos remanescentes florestais da Mata Atlântica. 1st edn. São Paulo.

Giannotti, E. & Machado, V.L.L. (1991). Notes on the foraging of two species of Ponerinae ants: Food resources and daily hunting activities (Hymenoptera, Formicidae). Bioikos, 5, 7–17.

GraphPad Software. (2017). GraphPad Prism 7.00 for Windows.

Instituto Chico Mendes de Conservação da Biodiversidade. (2018). Livro Vermelho da Fauna Brasileira Ameaçada de Extinção: Volume I. 1st edn. Instituto Chico Mendes de Conservação da Biodiversidade, Brasília.

Jones, J.C. & Oldroyd, B.P. (2006). Nest thermoregulation in social insects. Advances in Insect Physiology, 33, 153–191. DOI:

Kempf, W.W. (1971). A preliminary review of the ponerine ant genus Dinoponera Roger (Hymenoptera: Formicidae). Studia Entomologica, 14, 369–394.

Kierulff, M.C.M., Avelar, L.H.S., Ferreira, M.E.S., Povoa, K.F. & Bérnils, R.S. (2014). Reserva Natural Vale: História e aspectos físicos. Ciência & Ambiente, 49, 7–40.

Lenhart, P., Dash, S.T. & Mackay, W.P. (2013). A revision of the giant Amazonian ants of the genus Dinoponera (Hymenoptera, Formicidae). Journal of Hymenoptera Research, 31, 119–164. DOI:

MacArthur, R.H. & Pianka, E.R. (1966). On optimal use of a patchy environment. The American Naturalist, 100, 603–609. DOI:

Magnago, L.F.S., Rocha, M.F., Meyer, L., Martins, S.V. & Meira-Neto, J.A.A. (2015). Microclimatic conditions at forest edges have significant impacts on vegetation structure in large Atlantic Forest fragments. Biodiversity and Conservation, 24, 2305–2318. DOI:

Mariano, C.S.F., Pompolo, S.D.G., Barros, L.A.C., Mariano-Neto, E., Campiolo, S. & Delabie, J.H.C. (2008). A biogeographical study of the threatened ant Dinoponera lucida Emery (Hymenoptera: Formicidae: Ponerinae) using a cytogenetic approach. Insect Conservation and Diversity, 1, 161–168. DOI:

May, M.L. (1979). Insect thermoregulation. Annual Review of Entomology, 24, 313–349. DOI:

Medeiros, J., Azevedo, D.L.O., Santana, M.A.D., Lopes, T.R.P. & Araújo, A. (2014). Foraging activity rhythms of Dinoponera quadriceps (Hymenoptera: Formicidae) in its natural environment. Journal of Insect Science, 14, 1–9. DOI:

Ministério do Meio Ambiente. (2014). Lista nacional oficial de espécies da fauna ameaçada de extinção. Portaria no 444, de 17 de dezembro de 2014. Diário Oficial da União. Brasil.

Morgan, R.C. (1993). Natural history notes and husbandry of the Peruvian giant ant Dinoponera longipes (Hymenoptera: Formicidae). In: SASI/ITAG Conference Tucson, Arizona, August 13-15, 1993. pp. vii, 140–151.

Norberg, R.A. (1977). An ecological theory on foraging time and energetics and choice of optimal food-searching method. The Journal of Animal Ecology, 46, 511–529. DOI:

Paiva, R.V.S. & Brandão, C.R.F. (1995). Nests, worker population, and reproductive status of workers, in the giant queenless ponerine ant Dinoponera Roger (Hymenoptera Formicidae). Ethology Ecology and Evolution, 7, 297–312. DOI:

Peixoto, A.L., Rosa, M.M.T. & Joels, L.C.M. (1995). Diagramas de perfil e de cobertura de um trecho da floresta de tabuleiro na Reserva Florestal de Linhares (Espírito Santo, Brasil). Acta Botanica Brasilica, 9, 177–193. DOI:

Peixoto, A.V., Campiolo, S. & Delabie, J.H.C. (2010). Basic ecological information about the threatened ant Dinoponera lucida Emery (Hymenoptera: Formicidae: Ponerinae), aiming its effective long-term conservation. In: Species Diversity and Extinction (ed. Tepper, G.H.). Nova Science Publishers, Inc., New York., pp. 183–213.

Peixoto, A.V., Campiolo, S., Lemes, T.N., Delabie, J.H.C. & Hora, R.R. (2008). Comportamento e estrutura reprodutiva da formiga Dinoponera lucida Emery (Hymenoptera, Formicidae). Revista Brasileira de Entomologia, 52, 88–94. DOI:

Perry, G. & Pianka, E.R. (1997). Animal foraging: past, present and future. Trends in Ecology & Evolution, 12, 360–364. DOI:

Pincebourde, S. & Suppo, C. (2016). The vulnerability of tropical ectotherms to warming is modulated by the microclimatic heterogeneity. Integrative and Comparative Biology, 56, 85–97. DOI:

Pyke, G.H. (1984). Optimal foraging theory: A critical review. Annual Review of Ecology and Systematics, 15, 523–575. DOI:

Resende, H.C., Yotoko, K.S.C., Delabie, J.H.C., Costa, M.A., Campiolo, S., Tavares, M.G., et al. (2010). Pliocene and Pleistocene events shaping the genetic diversity within the central corridor of the Brazilian Atlantic Forest. Biological Journal of the Linnean Society, 101, 949–960. DOI:

Ribeiro, L.B., Ribeiro, M.G. & Freire, E.M.X. (2011). Hemidactylus brasilianus (Amaral’s Brazilian gecko) and Cnemidophorus ocellifer predation. Herpetological Bulletin, 117, 31–32.

Santos, R.J., Koch, E.B.A., Leite, C.M.P., Porto, T.J. & Delabie, J.H.C. (2017). An assessment of leaf-litter and epigaeic ants (Hymenoptera: Formicidae) living in different landscapes of the Atlantic Forest biome in the state of Bahia, Brazil. Journal of Insect Biodiversity, 5, 1–19. DOI:

Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., et al. (2012). Fiji: an open-source platform for biological-image analysis. Nature Methods, 9, 676–682. DOI:

Schneider, C.A., Rasband, W.S. & Eliceiri, K.W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9, 671–675. DOI:

Silvestre, R., Souza, P.R., Silva, G.S., Trad, B.M. & Lopez, V.M. (2015). Notas sobre interações competitivas envolvendo formigas poneromorfas. In: As formigas poneromorfas do Brasil (eds. Delabie, J.H.C., Feitosa, R.M., Serrão, J.E., Mariano, C.S.F. & Majer, J.D.). Editus, Ilhéus, BA, pp. 173–193. DOI:

Simon, S.S., Schoereder, J.H. & Teixeira, M.C. (2020). Environmental response of Dinoponera lucida Emery 1901 (Hymenoptera: Formicidae), an endemic threatened species of the Atlantic Forest Central Corridor. Sociobiology, 67, 65–73. DOI:

Simon, S.S., Teixeira, M.D.C. & Salomão, T. (2016). The reallocation of the ant species Dinoponera lucida Emery (Formicidae: Ponerinae) population increasing its local genetic diversity. Sociobiology, 63, 1058. DOI:

Sousa, P.A.G. & Freire, E.M.X. (2010). Coleodactylus natalensis (NCN) predation. Herpetological Review, 41, 218.

Stangler, E.S., Hanson, P.E. & Steffan-Dewenter, I. (2015). Interactive effects of habitat fragmentation and microclimate on trap-nesting Hymenoptera and their trophic interactions in small secondary rainforest remnants. Biodiversity and Conservation, 24, 563–577. DOI:

Welch, L.E., Baudier, K.M. & Harrison, J.F. (2020). Warmer mid-day temperatures increase leaf intake by increasing forager speed and success in Atta colombica during the rainy season. Insectes Sociaux, 67, 213–219. DOI:

Willmer, P.G. (1982). Microclimate and the Environmental Physiology of Insects. In: Advances in insect physiology (eds. Berridge, M.J., Treherne, J.E. & Wigglesworth, V.B.). Academic Press, London, UK, pp. 1–57. DOI:

Willmer, P.G. & Corbet, S.A. (1981). Temporal and microclimatic partitioning of the floral resources of Justicia aurea amongst a concourse of pollen vectors and nectar robbers. Oecologia, 51, 67–78. DOI:

Ydenberg, R.C. (2007). Provisioning. In: Foraging: Behavior and ecology (eds. Stephens, D.W., Brown, J.S. & Ydenberg, R.C.). The University of Chicago Press, Chicago, pp. 273–303.




How to Cite

Curbani, F., Zocca, C., Ferreira, R. B., Waichert, C., Sobrinho, T. G., & Srbek-Araujo, A. C. (2021). Litter Surface Temperature: A Driving Factor Affecting Foraging Activity in Dinoponera lucida (Hymenoptera: Formicidae). Sociobiology, 68(1), e6030.



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