Defensive repertoire of the stingless bee Melipona flavolineata Friese (Hymenoptera: Apidae)

Authors

  • Túlio Marcos Nunes NPPNS, Departamento de Física e Química, FCFRP, Universidade de São Paulo, Brazil
  • Lucas Garcia von Zuben Departamento de Biologia, FFCLRP, Universidade de São Paulo, Brazil
  • Luciano Costa Empresa Brasileira de Pesquisa Agropecuária - Embrapa Amazônia Oriental, Belém, Pará, Brazil.
  • Giorgio Cristino Venturieri Empresa Brasileira de Pesquisa Agropecuária - Embrapa Amazônia Oriental, Belém, Pará, Brazil.

DOI:

https://doi.org/10.13102/sociobiology.v61i4.541-546

Keywords:

Meliponini, pheromones, mandibular gland, Lestrimelitta limao

Abstract

Despite the fact that Meliponini bee lost their sting apparatus (stingless bees), they did not lose their ability to defend themselves. A variety of defensive strategies is described for the group, such as bitting and resin deposition. Defensive behaviours are usually mediated by chemical communication such as alarm pheromones. This study describe the defensive strategies of the stingless bee Melipona flavolineata Friese 1900 towards known pheromone sources, the head secretions of the robber bee Lestrimelitta limao and the mandibular gland extract of conspecifics M. flavolineata workers. The pheromones provoked different defensive reactions. The head secretions of the robber bee repelled returning foragers, elicited the enclosing of the nest entrance tube with batumen balls and agglomeration of workers outside of the box. In contrast, the mandibular gland extract elicited aggression towards the pheromone site, transport of resin and generalised agitated flights. The results confirm the mandibular gland as a source of alarm pheromone for this specie and also the chemical triggering of defensive response for the known cleptoparasite L. limao.

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References

Camargo, J.M.F. & Pedro, S.R.M. (2007). Meliponini Lepeletier, 1836. In J.S. Moure, J.S., D. Urban & G.A.R. Melo (Eds), Catalogue of bees (Hymenoptera, Apoidea) in the Neotropical region. Curitiba: Sociedade Brasileira de Entomologia.

Contrera, F.A.L., Menezes, C. & Venturieri, G.C. (2011). New horizons on stingless beekeeping (Apidae, Meliponini). Rev. Bras. Zootecn. 40: 48-51.

Cortopassi-Laurino, M., Imperatriz-Fonseca, V.L., Roubik, D.W., Dollin, A., Heard, T., Aguilar, I., Venturieri, G.C., Eardley, C. & Nogueira-Neto, P. (2006). Global meliponiculture: challenges and opportunities. Apidologie 37: 275-292.

Couvillon, M.J., Wenseleers, T., Imperatriz-Fonseca, V.L., Nogueira- Neto, P. & Ratnieks, F.L.W. (2008). Comparative study in stingless bees (Meliponini) demonstrates that nest entrance size predicts traffic and defensivity. J. Evolution. Biol. 21: 194-201. doi: 10.1111/j.1420- 9101.2007.01457.x

Cruz & López, L., Aguilar, S., Malo, E., Rincón, M., Guzman, M. & Rojas, J.C. (2007). Electroantennogram and behavioral responses of workers of the stingless bee Oxytrigona mediorufa to mandibular gland volatiles. Entomol. Exp. Appl. 123(1): 43-47. doi: 10.1111/j.1570-7458.2007.00522.x

Greco, M.K., Hoffmann, D., Dollin, A., Duncan, M., Spooner- Hart, R. & Neumann, P. (2010). The alternative Pharaoh approach: stingless bees mummify beetle parasites alive. Naturwissenschaften 97(3): 319-323. doi: 10.1007/s00114-009-0631-9

Grüter, C., Menezes, C., Imperatriz-Fonseca, V.L. & Ratnieks, F.L.W. (2012). A morphologically specialized soldier caste improves colony defense in a neotropical eusocial bee. P. Natl. Acad. Sci. USA 109: 1182-1186. doi: 10.1073/pnas.1113398109

Johnson, L.K., Haynes, L.W., Carlson, M.A., Fortnum, H.A. & Gorgas, D.L. (1985). Alarm substances of the stingless bee, Trigona silvestriana. J. Chem. Ecol. 11: 409-416. doi: 10.1007/BF00989552.

Kerr, W.E. (1951). Bases para o estudo da genética de populações dos Hymenoptera em geral e dos Apinae sociais em particular. An. ESALQ 8: 219-354.

Magalhães, T.L. & Venturieri, G.C. (2010). Aspectos econômicos da criação de abelhas indígenas sem ferrão (Apidae: Meliponini) no nordeste paraense. Série documentos EMBRAPA 364: 1-36.

Michener, C.D. (1946). Notes on Panamanian species of stingless bees. J. New York Entomol. S. 54: 179-197.

Michener, C.D. (2000). The bees of the world. Baltimore: John Hopkins University Press.

Nogueira-Neto, P. (1997). Vida e criação de abelhas indígenas sem ferrão. São Paulo: Nogueirapis.

Nunes, T.M., Nascimento, F.S., Turatti, I.C., Lopes, N.P. & Zucchi, R. (2008). Nestmate recognition in a stingless bee: does the similarity of chemical cues determine guard acceptance? Anim. Behav. 75:1165- 1171. doi: 0.1016/j.anbehav.2007.08.028

Oliveira, P.S., Müller, R.C.S., Dantas, K.D.G.F., Alves, C.N., Vasconcelos, M.A.M.D. & Venturieri, G.C. (2012). Phenolic acids, flavonoids and antioxidant activity in honey of Melipona fasciculata, M. flavolineata (Apidae, Meliponini) and Apis mellifera (Apidae, Apini) from the Amazon. Quim. Nova 35: 1728-1732.

Portugal-Araujo, V.D. (1978). Um artefato de defesa em colônias de Meliponineos. Acta Amaz.

Roubik, D.W., Smith, B.H. & Carlson, R.G. (1987). Formic acid in caustic cephalic secretions of stingless bee, Oxytrigona (Hymenoptera: Apidae). J. Chem. Ecol., 13: 1079-1086. doi: 10.1007/BF01020539.

Sakagami, S.F., Roubik, D.W. & Zucchi, R. (1993). Ethology of the robber stingless bee, Lestrimelitta limao (Hymenoptera: Apidae). Sociobiology 21: 237-277.

Sakagami, S.F. (1982) Stingless bees. In H.R. Hermann (Ed.), Social Insects (pp. 361-423), New York: Academic Press.

Sakagami, S.F. & Laroca, S. (1963). Additional Observations on the habits of the cleptobiotic stingless bees, the genus Lestrimelitta Friese (Hymenoptera, Apoidea). J. Fac. Sci. Hokkaido Univ. Series Zool. 15: 319-339.

Schorkopf, D.L.P., Hrncir, M., Mateus, S., Zucchi, R., Schmidt, V.M. & Barth, F.G. (2009). Mandibular gland secretions of meliponine worker bees: further evidence for their role in interspecific and intraspecific defence and aggression and against their role in food source signalling. J. Exp. Biol. 212: 1153- 1162. doi: 10.1242/jeb.021113.

Smith, B.H. & Roubik, D.W. (1983). Mandibular glands of stingless bees (Hymenoptera: Apidae): Chemical analysis of their contents and biological function in two species of Melipona. J. Chem. Ecol. 9: 1465-1472.

Venturieri, G.C., Raiol, V.D.F.O. & Pereira, C.A.B. (2003). Avaliação da introdução da criação racional de Melipona fasciculata (Apidae: Meliponina), entre os agricultores familiares de Bragança-PA, Brasil. Biota Neotropica 3: 1-7.

Venturieri, G.C. (2008) Caixa para a criação de uruçu-amarela Melipona flavolineata Friese, 1900. Embrapa Amazônia Oriental. Comunicado técnico.

Venturieri, G.C. (2008). Criação de abelhas indígenas sem ferrão. Embrapa Amazônia Oriental. 2nd Ed.

Zuben, L.G. (2012) Determinantes bionômicos e eco-químicos do cleptoparasitismo de Lestrimelitta limao Smith (Hymenoptera: Apidae, Meliponini), Monographs: Universidade de São Paulo.

Wilson, E.O. (1971) The insect societies. Cambridge: Harvard University Press.

Wittmann, D. (1985) Aerial defense of the nest by workers of the stingless bee Trigona (Tetragonisca) angustula (Latreille) (Hymenoptera: Apidae). Behav. Ecol. Sociobiol. 16: 111-114. doi: 10.1007/BF00295143

Wittmann, D., Radtke, R., Zeil, J., Lübke, G. & Francke, W. (1990). Robber bees (Lestrimelitta limao) and their host chemical and visual cues in nest defense by Trigona (Tetragonisca) angustula (Apidae: Meliponinae). J. Chem. Ecol. 16: 631-641. doi: 10.1007/BF01021793.

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Published

2014-12-29

How to Cite

Nunes, T. M., von Zuben, L. G., Costa, L., & Venturieri, G. C. (2014). Defensive repertoire of the stingless bee Melipona flavolineata Friese (Hymenoptera: Apidae). Sociobiology, 61(4), 541–546. https://doi.org/10.13102/sociobiology.v61i4.541-546

Issue

Vol. 61 No. 4 (2014)

Section

Research Article - Bees

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