Molecular Marker to Identify Two Stingless Bee Species: Tetragonisca angustula and Tetragonisca fiebrigi (Hymenoptera, Meliponinae)


  • Ana Lúcia Paz Barateiro Stuchi Universidade Estadual de Maringá
  • Vagner de A. Arnaut de Toledo Universidade Estadual de Maringá
  • Denise Alves Lopes Universidade Estadual de Maringá
  • Liriana Belizário Cantagalli Universidade Estadual de Maringá
  • Maria Claudia Colla Ruvolo-Takasusuki Universidade Estadual de Maringá Departamento de Biotecnologia Genética e Biologia Celular



stingless bees, isoenzymes, inhibition, thermostability, taxonomy


Tetragonisca angustula and T. fiebrigi esterases were biochemically characterized by their inhibition pattern and thermostability. Workers of both species were collected from nests at the State University of Maringa. In T. fiebrigi three esterases were observed: EST-1 (β-esterase, cholinesterase I), EST-2 (α-esterase, cholinesterase II) and EST-4 (αβ-esterase, carboxylesterase). In T. angustula two esterases were detected: EST-3 (β-esterase, acetylesterase) e EST-4 (αβ-esterase, carboxylesterase). T. angustula EST-3 showed the highest thermostability, and it was not observed above 54°C, while in T. fiebrigi EST- 1 and EST-2 were not detected above 52°C. Through this characterization, it was observed that EST-4 of T. angustula and T. fiebrigi showed identical biochemical characteristics, and probably those esterases are encoded by the same gene in the two species. Together, the biochemical characterization and molecular markers show that the two species are differentiated and secondary contact between the populations can still be occurring. 


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Author Biography

Maria Claudia Colla Ruvolo-Takasusuki, Universidade Estadual de Maringá Departamento de Biotecnologia Genética e Biologia Celular

Associate professor at the State University of Maringa, Department of Biotechnology, Genetics and Cell Biology.
Develop research in Population genetics of social insects (bees), gene expression in bees and pollination.


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How to Cite

Stuchi, A. L. P. B., Toledo, V. de A. A. de, Lopes, D. A., Cantagalli, L. B., & Ruvolo-Takasusuki, M. C. C. (2014). Molecular Marker to Identify Two Stingless Bee Species: Tetragonisca angustula and Tetragonisca fiebrigi (Hymenoptera, Meliponinae). Sociobiology, 59(1), 123–134.




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