Quality of Honey Produced by Four Species Of Stingless Bees in the Central Region of the State of Tocantins
DOI:
https://doi.org/10.13102/sociobiology.v69i2.7322Keywords:
native bees, honey production, physicochemical analysisAbstract
Meliponiculture, the rational breeding of native stingless bees, is considered an excelente sustainable alternative to assist in the pollination process and is an economically viable activity. In the cerrado of Tocantins, the meliponine species that stand out most due to their wide distribution are: Scaptotrigona tubiba, Melipona fasciculata,M. rufiventris and Tetragonisca angustula. The bibliographic collection about these species is still little explored, hence there is a need for research to deepen the existing knowledge in the area. For this reason, the aim of this study was: a) to quantify the honey production of four meliponine species: T. angustula, M. fasciculata, M. rufiventris, and S. tubiba; b) to determine the physicochemical characteristics of the product; c) measure the biological parameters of the colony and d) evaluate the profile and sensory acceptance of honey in the municipalities, Palmas and Miracema, in the Tocantins. The study evaluated the biological parameters of the colony, honey production, and physicochemical analysis. The highest honey production came from the species T. angustula in the two collections for the municipality of Palmas. For Miracema, the species S. tubiba and M. fasciculata were evaluated, respectively. The physicochemical parameters evaluated fit the norms assigned to honey quality control. Results showed that honey from M. fasciculata was the sensory profile that obtained the best average among the characteristics observed in the study. There was a positive and negative correlation between the biological parameters, with a significant difference only between the characters’ height and diameter of the honey pot.
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References
Almeida-Muradian, L.B., Stramm, K.M., Horita, A., Barth, O.M., Freitas, A.S., Estevinho, L.M. (2013). Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis mellifera. International Journal Food Science Technology, 48: 1698-1706. doi: 10.1111/ijfs.12140.
Alves, R.M.O., Souza, B.A. & Carvalho, C.A.L. (2007). Notas sobre a bionomia de Melipona mandaçaia (Apidae: Meliponini). Magistra, 19: 177-264. doi: 10.1590/1676-06032015009714.
Alves, R.M.O., Carvalho, C.A.L. & Faquinello, P. (2012). Parâmetros biométricos e produtivos de colônias de Melipona scutellaris Latreille, 1811 (Hymenoptera: Apidae) em diferentes gerações. Magistra, 24: 05-111.doi:10.13140/RG.2.1.2447.9768.
Anacleto, D.D.A., Souza, B.D.A., Marchini, L.C. & Moreti, A.C.D.C.C. (2009). Composição de amostras de mel de abelha Jataí (Tetragonisca angustula Latreille, 1811). Ciência e Tecnologia de Alimentos, 29: 535-541. doi: 10.1590/S0101-20612009000300013.
Antonini, Y., Martins, R.P., Aguiar, L.M. & Loyola, R.D. (2012). Richness, composition and trophic niche of stingless bee assemblage in urban forest remnants. Urban Ecosystems, 16: 527-541. doi: 10.1007/s11252-012-0281-0.
Araújo, J.S., Chambó, E.D., Costa, M.A.P.C., Cavalcante, D.A., Silva, S.M.P., Carvalho, C.A.L. & Estevinho, L.M. (2017). Chemical composition and biological activities of monoand heterofloral bee pollen of different geographical origins. International Journal of Molecular Science, 18: e921. doi: 10.3390/ijms18050921.
Azeredo, L.C., Azeredo, M.A.A. & Beser, L.B.O. (2000). Características físico-químicas de amostras de méis de melíponas coletadas no Estado de Tocantins. In: Congresso Brasileiro de Apicultura. Florianópolis. doi: 10.1590/S0101-20612005000400004.
Bartelli, B.F. & Nogueira-Ferreira, F.H. (2014). Pollination services provided by Melipona quadrifasciata Lepeletier (Hymenoptera: Meliponini) in greenhouses with Solanum lycopersicum L. (Solanaceae). Sociobiology, 61: 510-516. doi: 10.13102/sociobiology.v61i4.510-516.
Braghini, F. (2016). Estabilidade de méis de abelhas sem ferrão (Meliponinae spp.) submetidos a diferentes condições térmicas. 156f. Dissertação (Mestrado) Programa de Pós-Graduação em Ciências dos Alimentos do Centro de Ciências Agrárias – Universidade Federal de Santa Catarina, Florianópolis-SC.
Brasil. Instrução Normativa 11 de 20 de outubro de 2000. Regulamento Técnico de Identidade e Qualidade do Mel. Diário Oficial da União. Legislação de Produtos Apícolas Derivados. Abastecimento, p. 23.
Camargo, R.C.R., Oliveira, K.L. & Berto, M.I. (2017). Mel de abelhas sem ferrão: proposta de regulamentação. Brazilian Journal of Food Technology, 20: e2016157. doi: 10.1590/1981-6723.15716.
Carvalho, C.A.L., Alves, R.M.O., Souza, B.A., Véras, S.O., Alves, E.M. & Sodré, G.S. (2013). Proposta de regulamento técnico de qualidade físico-química do mel floral processado produzido por abelhas do gênero Melipona. In: Stingless bees process honey and pollen in cerumen pots. Eds: Vit, P. & Roubik, D.W., p. 1-9. doi: 10.1590/1981-6723.15716.
Carvalho-Zilse, G.A. (2012). Meliponicultura na Amazônia. Manaus: [s.n.], 50p.
Codex Alimentarius Commission (2001). Codex Alimentarius Commission Standards. Codex Stan 12-1981, p. 1-8.
Costa, A.C.V., Sousa, J.M.B., Silva, M.A.A.P., Garruti, D.S. & Madruga, M.S. (2017). Sensory and volatile profiles of monofloral honeys produced by native stingless bees of the Brazilian semiarid region. Food Research International, 105: 110-120. doi: 10.1016/j.foodres.2017.10.043
Crane, E. (1985). O livro do mel, Livraria Nobel, São Paulo.
DaSilva, P.M., Gauche, C., Gonzaga, L.V., Costa, A.C.O. & Fett, R. (2016). Honey: Chemical composition, stability and authenticity. Food Chemistry, 196: 309-323. doi: 10.1016/j.foodchem.2015.09.051.
Evangelista-Rodrigues, A., Góis, G.C., Silva, C.M. Souza, D.L., Souza, D.N., Silva, P.C.C., Alves, E.l. & Rodrigues, M. L. (2008). Desenvolvimento produtivo de colméias de abelhas Melipona scutellaris. Revista Biotemas, 21: 59-64. doi: 10.5007/2175-7925.2008v21n1p59
Faquinello, P., Brito, B.B.P., Carvalho, C.A.L., Paula-Leite, M.C. & Alves, R.M.O. (2013). Correlação entre parâmetros biométricos e produtivos em colônias de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera: Apidae). Ciência Animal Brasileira, 14: 312-317. doi: 10.5216/cab.v14i3.18603.
Ferreira, E.L., Lencioni, C., Benassi, M.T., Barth, M.O. & Bastos, D.H.M. (2009). Descriptive sensory analysis and acceptance of stingless bee honey. Food Science and Technology International, 15: 251-258. doi: 10.1177/1082013209341136.
Ferrufino, U. & Vit, P. (2013). Pot-honey of six Meliponines from Amboro National Park, Bolivia. In: Vit, P.; Pedro, S.R.M. & Roubik, D. Pot-honey: a legacy of stingless bees. New York: Springer. p. 409-416. doi: 10.1007/978-1-4614-4960-7_29.
Guerrini, A., Bruni., R., Maietti, S., Poli, F., Rossi, D., Paganetto, G., Muzzoli, M., Scalvenzi, L. & Sacchetti, G. (2009). Ecuadorian stingless bee (Meliponinae) honey: A chemical and functional profile of an ancient health product. Food Chemistry, 114: 1413-1420. doi: 10.1016/j.foodchem.2008.11.023.
Hilário, S.D., Imperatriz-Fonseca, V.L. & Kleinert, A.M.P. (2001). Responses to climatic factors by foragers of Plebeia pugnax Moure (in litt.) (Apidae, Meliponinae). Revista Brasileira de Biologia, 61: 191-196. doi: 10.1590/S0034-7108 2001000200003.
IHC (1997). Harmonized methods of the International Honey Commission. IHC responsible for the methods: Stefan. Anals. p. 10-16.
Lacerda, J.J.J., Santos, J.S., Santos, A.S., Rodrigues, G.B. & Santos, M.L.P. (2010). Influência das características físico-químicas e composição elementar nas cores de méis produzidos por Apis mellífera no sudoeste da Bahia utilizando análise multivariada. Química Nova, 33: 1022-1026. doi: 10.1590/S0100-40422010000500003.
Larana (1981). Laboratório Nacional de Referência Animal. Métodos analíticos oficiais para controle de produção, controle de produtos de origem animal e seus ingredientes. II - Métodos físicos e químicos. Mel. Brasília: Ministério da Agricultura, v.2, cap. 25, p. 1-15.
Lira, A.F., Sousa, J.P.L.M., Lorenzon, M.C.A.,Vianna, C.A.F.J. & Castro, R.N. (2014). Estudo comparativo do mel de Apis mellífera com méis de meliponíneos. Acta Veterinaria Brasilica, 8: 169-178. doi: 10.21708/AVB.2014.8.3.3560.
Michener, C.D. (2000). The bees of the world. Johns Hopkins Univ. Press, p. 872.
Migliato, K.F. (2005). Syzygium cumini (L.) Skeels - Jambolão: estudo farmacognóstico, otimização do processo extrativo, determinação da atividade antimicrobiana do extrato e avaliação da atividade antisséptica de um sabonete líquido contendo o referido extrato. Dissertação de Mestrado em Ciências Farmacêuticas. Araraquara, 179 p.
Moraes, R.M. de & Texeira, E.W. (1998). Análise de mel. Pindamonhangaba: Instituto de Zootecnia. Manual Técnico, 41 p.
Nascimento, A.S., Marchini, L.C., Carvalho, C.A.L., Araújo, D.F.D., Olinda, R.A. & Silveira, T.A. (2015). Physical-Chemical Parameters of Honey of Stingless Bee (Hymenoptera: Apidae). Chemical Science International Journal, 7: 139-149. doi: 10.9734/ACSJ/2015/17547.
Pereira, F.M. et al. (2012). Manejo de colônias de abelhas-sem-ferrão. Teresina: Embrapa Meio-Norte. 31p.
Pereira, F.M., Souza, B.A. & Lopes, M.T.R. (2021). Criação de abelhas sem ferrão. http://ainfo.cnptia.embrapa.br/digital/bitstream/item/166288/1/CriacaoAbelhaSemFerrao.pdf. Acessed: January 2021.
Ramón-Sierra, J.M., Ruiz-Ruiz, J.C. & Ortiz-Vázquez, E.D.L.L. (2015). Electrophoresis characterization of protein as a method to establish the entomological origin of stingless bee honeys. Food Chemistry, 183: 43-48. doi: 10.1016/j.foodchem.2015.03.015.
Ribeiro, M.F., Santos-Filho, P.S. & Imperatriz-Fonseca, V.L. (2006). Size variation and egg laying performance in Plebeia remota queens (Hymenoptera, Apidae, Meliponini). Apidologie, 37: 191-206. doi: 10.1051/apido:2006046.
Ribeiro, R.O.R., Mársico, E.T., Carneiro, C.S., Monteiro, M.L.G., Júrnior, C.A.C., Mano, S. & Jesus, E.F.O. (2014). Classification of Brazilian honeys by physical and chemical analytical methods and low field nuclear magnetic resonance. LWT – Food Science and Technology, 55: 90-95. doi: 10.1016/ j.lwt.2013.08.004.
Rodrigues, C.S., Ferasso, D.C., Prestes, O.D., Zanella, R., Grando, R.C., Treichel, H., Coelho, G.C. & Mossi, A.J. (2018). Quality of Meliponinae honey: Pesticides residues, pollen identity, and microbiological profiles. Environmental Quality Management, 27: 39-45. doi: 10.1002/tqem.21547.
Roubik, D.W. (2018a). The pollination of cultivated plants. A compendium for practitioners. Vols. 1 & 2. (Ed.) Rome: FAO, 324 p.
Se, K.W., Ibrahim, R.K.R.,Wahab, R.A. & Ghoshal, S.K. (2018). Accurate evaluation of sugar contents in stingless bee (Heterotrigona itama) honey using a swift scheme. Journal of Food Composition and Analysis, 66: 46-54. doi: 10.1016/j.jfca.2017.12.002.
Shadan, A.F., Mahat, N.A.,Wan Ibrahim, W.A., Ariffin, Z. & Ismail, D. (2018). Provenance establishment of stingless bee honey using multi-element analysis in combination with chemometrics techniques. Journal of Forensic Sciences, 63: 80-85.doi: 10.1111/1556-4029.13512.
Silva, A.S., Alves, C.N., Fernandes, K.G. & Müller, R.C.S. (2013). Classification of honeys from Pará State (Amazon region, Brazil) produced by three different species of bees. Journal Brazilian Chemical Society, 24: 1135-1145. doi: 10.5935/0103-5053.20130147.
Silveira, F.A., Melo, G.A.R., Campos, L.A.O., Marini Filho, O.J., Menezes-Pedro, S.R. (2018). Melipona rufiventris Lepeletier, 1836. In: ICMBio (Org), Livro vermelho da fauna ameaçada de extinção.V. 7. Invertebrados.
Singh, A.K. (2016). Traditional Meliponiculture by Nagha tribes in Nagaland, India. Indian Journal of Traditional Knowledge, 154: 693-699.
Sodré, G.S., Carvalho, C.A.L., Fonseca, A.A.O., Alves, R.M.O. & Souza, B.A. (2008). Perfil sensorial e aceitabilidade de méis de abelhas sem ferrão submetidos a processos de conservação. Ciência e Tecnologia dos Alimentos, 28: 72-77. doi: 10.1590/S0101-20612008000500012
Sousa, J.M.B., Souza, I.A., Magnani, M. & Albuquerque, J.R. (2013). Physicochemical aspects and sensory profile of stingless bee honeys from Seridó region, State of Rio Grande do Norte, Brazil. Semina: Ciências Agrárias, 34: 1765-1774. doi: 10.54 33/1679-0359.2013v34n4p1765
Sousa, J.M.B., Souza, E.L., Marques, G., Benassi, M.T., Gullón, B., Pintado, M.M. & Magnani, M. (2016). Sugar profile, physicochemical and sensory aspects of monofloral honeys produced by different stingless bee species in Brazilian semi-arid region. LWT-Food Science and Technology, 65: 645-651. doi: 10.1016/j.lwt.2015.08.058.
Souza, B., Roubik, D., Barth, O.,Heard, T., Enríquez, E., Carvalho, C., Villas-Bôas, J. Marchini, L.C., Locatelli, J., Persano-Oddo, L., Almeida-Muradian, L. Bogdanov, S. & Vit, P. (2006). Composition of stingless bee honey: Setting quality standards. Interciência, 31: 867-875.
Souza, S.G.X.,Teixeira, A.F.R., Neves, E.L. & Melo, A.M.C. (2005). As abelhas sem ferrão (Apidae: Meliponina) residentes no campus Federação/Ondina da Universidade Federal da Bahia, Salvador, Bahia, Brasil. Candombá. Revista Virtual, 1: 57-69.
Venturieri, G.C. (2008b). Criação de abelhas indígenas sem ferrão. Belém: Embrapa Amazônia Oriental, 60 p.
Villas-Bôas, J.K. & Malaspina, O. (2005). Parâmetros físico-químicos propostos para o controle de qualidade do mel de abelhas indígenas sem ferrão no Brasil. Mensagem Doce, 82: 6-16.
Vit, P. (2013). Melipona favosa Pot-Honey from Venezuela. In: Vit, P., Pedro, S.R.M. & Roubik, D.W. (Eds.); Pot-Honey A legacy of stingless bees. 1st ed., p. 363-373.
Vit, P., Pedro, S.R.M. & Roubik, D.W. (2013). PotHoney: A Legacy of Stingless Bees. Springer, N. Y. 655 p.
Zielinski, A.A.F., Ávila, S., Ito., V., Nogueira, A., Wosiacki, G., Windson, C. & Haminiuk, I. (2014). The association between chromaticity, phenolics, carotenoids, and in vitro antioxidant activity of frozen fruit pulp in Brazil: An application of chemometrics. Journal of Food Science, 79: C510-C516. doi: 10.1111/1750-3841.12389
Zuccato, V., Finotello, C., Menegazzo, I., Peccolo, G. & Schievano, E. (2017). Entomological authentication of stingless bee honey by 1 H NMR-based metabolomics approach. Food Control, 82: 145-153. doi: 10.1016/j.foodcont.2017.06.024
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