Suitability of Substrate for Laboratory Studies with the Subterranean Termite Reticulitermes grassei (Clément) (Isoptera: Rhinotermitidae)


  • Patricia Gallardo University of Córdoba
  • Ana M Cardenas Ana M. Cardenas University of Cordoba Spain termites, biology, foraging
  • Raquel Murillo TAGESA



Rearing cultures, Suitability of substrate, Termite survival rate


Two substrates, sand and vermiculite, were tested in rearing laboratory experiments with Reticulitermes grassei at different culture periods (2, 4 and 6 weeks). Although information is available regarding procedures to keep species of Reticulitermes in lab, none of them is referred to the Mediterranean termite R. grassei. The suitability of substratum was assessed in terms of survival of termites. The highest survival rate was in the experiment with sand and short-time duration, but after six weeks exposure, survival was significantly lower on this substrate. These differences among substrates were not significant for any of the treatments.


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Author Biography

Patricia Gallardo, University of Córdoba

Department of Zoology.


Alcaide, M.E. (2010). Estudio del control integral de una plaga urbana de termitas en el sur de España. Dissertation, Universidad de Córdoba, España.

Arinana, L., Tsunoda, K., Herliyana, E.N. & Hadi, Y.S. (2012). Termite-susceptible species of wood for inclusion as a reference in Indonesian standardized laboratory testing. Insects, 3: 396-401. doi: 10.3390/insects3020396.

Cárdenas, A.M., Moyano, L., Gallardo, P. & Hidalgo, J.M. (2012). Field activity of Reticulitermes grassei (Isoptera: Rhinotermitidae) in oak forests of the Southern Iberian Peninsula. Sociobiology, 59(1): 493-509. doi: 10.13102/sociobiology.v59i2613.

Clément, J.L., Bagnères, A.G., Uva, P., Wilfert, L., Quintana, A., Reinhard, J. & Dronnet, S. (2001). Biosystematics of Reticulitermes termites in Europe: morphological, chemical and molecular data. Insect. Soc., 48: 202-215. doi: 10.1007/PL00001768.

EN 118 (2013). Wood preservatives: Determination of preventive action against Reticulitermes species (European termites) (laboratory method). European Committee for Standardization, BSI Standards Publication, CEN-CENELEC, Brussels, Belgium.

Fougerousse, M. (1973). Some aspects of laboratory and field testing methods of antitermite wood preservatives. Holzforsch, 27(4): 137-142. doi: 10.1515/hfsg.1973.27.4.137.

Gaju, M., Notario, M.J., Mora, R., Alcaide, E., Moreno, T., Molero, R. & Bach, C. (2002). Termite damage to buildings in the province of Córdoba, Spain. Sociobiology, 40: 75-85.

Gaju, M., Moyano, L., Alcaide, E., Patiño, C., Diz, J., Nunes, L., Bach, C. & Molero, R. (2010). Laboratory study of fipronil baits against Reticulitermes grassei (Isoptera: Rhinotermitidae). Proceeding of 41st Annual Meeting the International Research group on wood protection, Biarritz, France.

Gallardo, P., Cárdenas, A.M. & Gaju, M. (2010). Ocurrence of Reticulitermes grassei (Isoptera: Rhinotermitidae) on cork oaks in the southern Iberian Peninsula: identification, description and incidence of the damage. Sociobiology, 56(3): 675-687.

Haverty, M.I. (1979). Selection of tunnelling substrates for laboratory studies with three subterranean termite species. Sociobiology, 4(3): 315-320.

Haverty, M.I. & Howard, R.W. (1981). Production of soldiers and maintenance of soldier proportions by laboratory experimental groups of Reticulitermes flavipes (Kolar) and Reticulitermes virginicus (Banks) (Isoptera: Rhinotermitidae). Insect. Soc., 28: 32-39. doi: 10.1007/BF02223620.

Ho, T.H. & Kirton, L.G. (2007). Effects of different substrates and activated charcoal on the survival of the subterranean termite Coptotermes curvignathus in laboratory experiments (Isoptera: Rhinotermitidae). Sociobiology, 50: 479-497.

Lenz, M. (2005). Laboratory bioassays with termites-the importance of termite biology. Proceeding the 2ª Conference of Pacific Rim Termite Research Group on Wood Protection, Bangkok, Thailand.

Munizaga, M.J. (2007). Estudio de alimentación y de sustancias tóxicas potenciales para Reticulitermes flavipes (Kollar) (Isoptera: Rhinotermitidae). Dissertation, University of Chile.

Munizaga, M.J., Araya, J.E., Karsulovic, T. & Bozo, A. (2008). ¿Afecta la exposición previa a ninfas el consumo de madera de pino radiata de Reticulitermes flavipes (Kollar) (Isoptera: Rhinotermitidae)?. Bol. San. Veg.: Plagas, 34: 533-536.

Nobre, T., Nunes, L. & Bignell, D.E. (2007). Tunnel geometry of the subterranean termite Reticulitermes grassei (Isoptera: Rhinotermitidae) in response to sand bulk density and the presence of food. Insect. Sci. 14: 511-518. doi: 10.1111/j.1744-7917.2007.00180.x

Perkins, G.D. (2012). Reticulitermes feeding preference and experimental design. Dissertation, University of Georgia.

Smythe, R.V. & Carter, F.L. (1970). Feeding responses to sound wood by Coptotermes formosanus, Reticulitermes flavipes, and R. virginicus (Isoptera: Rhinotermitidae). Ann. Entomol. Soc. Am., 63: 841-847. doi:

SPSS Inc. (2011). SPSS 20.0 for Windows Use Manual (version 20.0).




How to Cite

Gallardo, P., Cardenas, A. M., & Murillo, R. (2016). Suitability of Substrate for Laboratory Studies with the Subterranean Termite Reticulitermes grassei (Clément) (Isoptera: Rhinotermitidae). Sociobiology, 63(2), 855–857.



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