Induction and morphological and biochemical characterization of Hyptis leucocephala (Lamiaceae) calluses

Authors

  • Danilo Marcelo Santos Pereira
  • Lenaldo Muniz de Oliveira
  • Cristina Ferreira Nepomuceno
  • José Raniere Ferreira de Santana
  • Marília Lordelo Cardoso Silva
  • Maria Gabriela Bello Koblitz

DOI:

https://doi.org/10.13102/scb124

Abstract

Hyptis leucocephala is an aromatic herb endemic to the Brazilian semiarid region, with antimicrobial, antifungal,
cytotoxic, anti-inflammatory and anti-HIV properties. In vitro cultivation makes the production of high genetic and
sanitary quality cuttings possible for this species, allowing its sustainable exploitation. In the present study, the effect of 6-
benzilaminopurine (BAP) and naftalenoacetic acid (NAA) on callus induction in leaf segments of this species and
different organic additives to WPM (Wood Plant Medium) for the callus induction was evaluated. The combination of
BAP and NAA allowed the induction of compact and undifferentiated calluses. The highest rate of callus formation and
growth was obtained with the combination of 8.88 μM BAP and 21.48 μM NAA. Supplementing the culture medium with
coconut water, malt extract and yeast extract did not induce friable calluses. The levels of total sugars, reducing sugars,
protein, and amino acids were reduced in calluses maintained in culture medium supplemented with coconut water, malt
extract and yeast extract.

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Published

2012-06-27

How to Cite

Pereira, D. M. S., de Oliveira, L. M., Nepomuceno, C. F., de Santana, J. R. F., Silva, M. L. C., & Koblitz, M. G. B. (2012). Induction and morphological and biochemical characterization of Hyptis leucocephala (Lamiaceae) calluses. SITIENTIBUS série Ciências Biológicas, 12(1), 151–156. https://doi.org/10.13102/scb124

Issue

Vol. 12 No. 1 (2012)

Section

Artigos

License

Sitientibus série Ciências Biológicas (SCB) is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.