Intraspecific Variation of the Composition of Linear Alkanes in Social Wasp Mischocyttarus consimilis


  • Ana Cristina Ferreira Universidade Federal do Triangulo Mineiro
  • Erika Fernandes Neves Instituto Federal de Mato Grosso do Sul
  • Thiago dos Santos Montagna Universidade Estadual de Mato Grosso do Sul
  • Angélica Mendonça Universidade Federal da Grande Dourados Faculdade de Ciências Biológicas e Ambientais
  • Claudia Andrea Lima Cardoso Universidade Estadual de Mato Grosso do Sul
  • William Fernando Antonialli Junior Universidade Estadual de Mato Grosso do Sul



Chemical signature, castes, cuticular hydrocarbons, gas chromatography, population variation


An important attribute of the evolution and maintenance of sociality in insects is their ability to distinguish members of their own colonies by means of chemical signals during their interactions. Of this type of signals, the cuticular hydrocarbons, responsible among other functions, for intraspecific recognition stand out. Linear alkanes are indicated as the class of compounds that would be most involved in water retention in the body of insects, however, some studies have investigated their role as mediators of interactions. Thus it is possible that there is significant intraspecific variation of its composition, so the objective of this study was to test the hypothesis that linear alkanes vary significantly among populations, castes and sexes, as well as between newly emerged females of different ages of the Mischocyttarus consimilis Zikán 1949 wasp. The samples were analyzed by gas chromatography with flame ionization detector and gas chromatography coupled to mass spectrometry. The results show that there is a significant variation between the chemical cuticular profiles of samples from different populations, as well as between castes, sex and newly emergent workers of different ages. Therefore, it is possible to infer that this class of compounds may vary according to genetic differences between populations, but also by different environmental conditions. The differences between castes, sex and ages suggest that these compounds may also be involved in mediating interactions between nestmates.


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Abdalla, F. C., Jones, G. R., Morgan, E. D. & Cruz-Landim, C. (2003). Comparative study of the cuticular hydrocarbon composition of Melipona bicolor Lepeletier, 1836 (Hymenoptera, Meliponini) workers and queens. Genetics and Molecular Research, 2: 191-199.

Antonialli-Junior, W. F., Lima, S. M., Andrade, L. H. C. & Súarez, Y. R. (2007). Comparative study of the cuticular hydrocarbon in queens, workers and males of Ectatomma vizotti (Hymenoptera: Formicidae) by Fourier transform-infrared photoacoustic spectroscopy. Genetics and Molecular Research, 6: 492-499.

Antonialli-Junior, W. F., Súarez, Y. R., Izida, T., Andrade, L. H. C., & Lima, S. M. (2008). Intra-and interspecific variation of cuticular hydrocarbon composition in two Ectatomma species (Hymenoptera: Formicidae) based on Fourier transform infrared photoacoustic spectroscopy. Genetics and Molecular Research, 7: 559-566.

Arathi, H. S., Shakarad, M. & Gadagkar, R. (1997). Factors affecting the acceptance of alien conspecifics on nests of the primitively eusocial wasp, Ropalidia marginata (Hymenoptera: Vespidae). Journal of Insect Behavior, 10: 343-353.

Armold, M. T. & Regnier, F. E. (1975). A developmental study of the cuticular hydrocarbons of Sarcophaga bullata. Journal of Insect Physiology, 21: 1827–1833. doi:10.1016/0022-1910(75)90249-8

Bernardi, R. C., Firmino, E. L. B., Pereira, M. C., Andrade, L. H. C., Cardoso, C. A. L., Súarez, Y. R., Antonialli-Junior, W. F. & Lima, S. M. (2014). Fourier transform infrared photoacoustic spectroscopy as a potential tool in assessing the role of diet in cuticular chemical composition of Ectatomma brunneum. Genetics and Molecular Research, 13: 10035-10048.

Billen, J. & Morgan, D. (1998). Pheromone Comunication in social insects: sources and secretions. In Vander Meer, R. K., Breed, M., Winston, M. & Espelie, K. E. (Eds.), Pheromone comunication in social insects: ants, wasps, bees and termites (p. 3-33.). Westview Press: Bolder, CO.

Blomquist, G. & Bagnères, A. G. (2010). Insect hydrocarbons: biology, biochemistry and chemical ecology. Cambridge: Cambridge University Press, 506 p.

Bonavita-Cougourdan, A., Clement, J. L. & Lange, C. (1987). Nestmate recognition: the role of cuticular hydrocarbons in the ant Camponotus vagus Scop. Journal of Entomological Science, 22: 1–10.

Borges, A. A., Ferreira-Caliman, M. J., Nascimento, F. S., Campos, L. A. O., & Tavares, M. G. (2012). Characterization of cuticular hydrocarbons of diploid and haploid males, workers and queens of the stingless bee Melipona quadrifasciata. Insectes sociaux, 59: 479-486. doi:10.1007/s00040-012-0242-x

Butts, D. P., Camann, M. A. & Espelie, K. E. (1995). Workers and queens of the European hornet Vespa crabro L. have colony-specific cuticular hydrocarbon profiles (Hymenoptera: Vespidae). Insectes Sociaux, 42: 45-55. doi:10.1007/BF01245698

Chapman, R. F., Espelie, K. E. & Swords, G. A. (1995). Use of cuticular lipids in grasshopper taxonomy: A study of variation in Schistocerca shoshone (Thomas). Biochemical Systematics and Ecology, 23: 383–398. doi:10.1016/0305-1978(95)00032-P

Cotoneschi, C., Dani, F. R., Cervo, R., Scala, C., Strassmann, J. E., Queller, D. C., & Turillazzi, S. (2009). Polistes dominulus (Hymenoptera, Vespidae) larvae show different cuticular patterns according to their sex: workers seem not use this chemical information. Chemical Senses, 34: 195-202. doi:10.1093/chemse/bjn079

Dani, F. R., Morgan, E. D., & Turillazzi, S. (1996). Dufour gland secretion of Polistes wasp: chemical composition and possible involvement in nestmate recognition (Hymenoptera: Vespidae). Journal of Insect Physiology, 42: 541-548. doi:10.1016/0022-1910(95)00136-0

Dapporto, L., Theodora, P., Spacchini, C., Pieraccini, G., & Turillazzi, S. (2004a). Rank and epicuticular hydrocarbons in different populations of the paper wasp Polistes dominulus (Christ) (Hymenoptera, Vespidae). Insectes Sociaux, 51: 279-286. doi:10.1007/s00040-004-0738-0

Dapporto, L., Palagi, E., & Turillazzi, S. (2004b). Cuticular hydrocarbons of Polistes dominulus as a biogeographic tool: a study of populations from the Tuscan Archipelago and surrounding areas. Journal of chemical ecology, 30: 2139-2151. doi:10.1023/B:JOEC.0000048779.47821.38

Espelie, K. E., Butz, V. M., Dietz, A. (1990). Decyl decanoate: a major component of the tergite glands of honeybee queens (Apis mellifera L.). Journal of Apicultural Research, 29: 15–19. doi: 10.1080/00218839.1990.11101192

Ferreira, A. C., Cardoso, C. A. L., Neves, E. F., Súarez, Y. R., & Antonialli-Junior, W. F. (2012). Distinct linear hydrocarbon profiles and chemical strategy of facultative parasitism among Mischocyttarus wasps. Genetics and Molecular Research, 11: 4351-4359.

Gamboa, G.J., H.K. Reeve, I.D. Ferguson & T.L. Wacker, (1986). Nestmate recognition in social wasps: origin and acquisition of recognition odours. Animal Behavior, 34: 685–695.

Gamboa, G. J. (1996). Kin recognition in social wasps. In Turillazi, S. & West-Eberhard, M. J. (Eds.), Natural history and evolution of paper-wasps (pp 161-177). Oxford: University Press.

Gamboa, G. J. (2004). Kin recognition in eusocial wasps. In Annales Zoologici Fennici (pp. 789-808). Finnish Zoological and Botanical Publishing Board.

Gibbs, A. G. (1998). Water-proofing properties of cuticular lipids. American Zoologist, 38: 471–482. doi:10.1093/icb/38.3.471

Gibbs, A. G. (2002). Lipid melting and cuticular permeability: New insights into an old problem. Journal of Insect Physiology, 48: 391–400. doi:10.1016/S0022-1910(02)00059-8

Hefetz, A. (2007). The evolution of hydrocarbon pheromone parsimony in ants (Hymenoptera: Formicidae) – interplay of colony odor uniformity and odor idiosyncrasy. Myrmecological News, 10: 59-68.

Hora, R. R., Ionescu-Hirsh, A., Simon, T., Delabie, J., Robert, J., Fresneau, D., & Hefetz, A. (2008). Postmating changes in cuticular chemistry and visual appearance in Ectatomma tuberculatum queens (Formicidae: Ectatomminae). Naturwissenschaften,95: 55-60. doi:10.1007/s00114-007-0287-2

Le Moli, F., Mori, A. & Grasso, D. A. (1992). Nestmate and conspecific non nestmate recognition in Formica cunicularia Latr.: the effect of diet differences. In Billen, J. (Eds.) Biology and evolution of social insects (pp161–165). Leuven, Belgium: Leuven University Press.

Leal, W. S. (2005). Pheromone Reception. In Balzani, V., Houk, K. N., Kessler, H. & Lehn, J. M. (Eds.). Topics in Current Chemistry (1-36). Springer: Heidelberg.

LeConte, Y. & Hefetz, A. (2008). Primer pheromones in social Hymenoptera. Annual Review of Entomology, 53: 523–542. doi:10.1146/annurev.ento.52.110405.091434

Lenoir, A., Cuisset, D., Hefetz, A. (2001). Effects of social isolation on hydrocarbon pattern and nestmate recognition in the ant Aphaenogaster senilis (Hymenoptera, Formicidae). Insectes Sociaux 48:101-109. doi:10.1007/PL00001751

Lenoir, A., Depickere, S., Devers, S., Christides, J. P., & Detrain, C. (2009). Hydrocarbons in the ant Lasius niger: From the cuticle to the nest and home range marking. Journal Chemical Ecology, 35: 913–921. doi:10.1007/s10886-009-9669-6

Liang, D. & Silverman, J. (2000). “You are what you eat”: Diet modifies cuticular hydrocarbons and nestmate recognition in the Argentine ant, Linepithema humile. Naturwissenschaften, 87: 412-416. doi:10.1007/s001140050752

Lommelen, E., Johnson, C. A., Drijfhout, F. P., Billen, J., Wenseleers, T., Gobin, B. (2006). Cuticular hydrocarbons provide reliable cues of fertility in the ant Gnamptogenys striatula. Journal Chemical Ecology, 32: 2023–2034. doi:10.1007/s10886-006-9126-8

Lorenzi, M. C., Cometto, I. & Marchisio, G. (1999). Species and colony components in the recognition odor of young social wasps: their expression and learning (Polistes biglumis and P. atrimandibularis; Hymenoptera: Vespidae). Journal of Insect Behavior, 12: 147-158. doi:10.1023/A:1020906631121

Lorenzi, M. C., Sledge, M. F., Laiolo, P., Sturlini, E. & Turillazzi, S. (2004). Cuticular hydrocarbon dynamics in young adult Polistes dominulus (Hymenoptera: Vespidae) and the role of linear hydrocarbons in nestmate recognition systems. Journal of Insect Physiology, 50: 935-941. doi:10.1016/j.jinsphys.2004.07.005

Lorenzi, M. C., Azzani, L., & Bagnéres, A. G. (2014). Evolutionary consequences of deception: Complexity and informational content of colony signature are favored by social parasitism. Current zoology, 60: 137-148. doi:10.1093/czoolo/60.1.137

Montagna, T. S., Torres, V. O., Dutra, C. C., Suarez, Y. R., Antonialli Junior, W. F. & Junior, V. V. A. (2009). Study of the foraging activity of Mischocyttarus consimilis (Hymenoptera: Vespidae). Sociobiology, 53: 131-140.

Neves, E. F., Andrade, L. H. C., Súarez, Y. R., Lima, S. M., Antonialli-Junior, W. F. (2012). Age-related changes in the surface pheromones of the wasp Mischocyttarus consimilis (Hymenoptera: Vespidae). Genetics and Molecular Research, 11:1891-1898.

Neves, E. F., Montagna, T. S., Andrade, L. H. C., Súarez, Y. R., Lima, S. M., Antonialli-Junior, W. F. (2013). Social parasitism and dynamics of cuticular hydrocarbons in paper wasp of the Mischocyttarus. Journal of the Kansas Entomological Society, 86: 69-77. doi: 10.2317/JKES1207610.1

Nunes, T. M., Turatti, I. C. C., Mateus, S., Nascimento, F. S., Lopes, N. P. & Zucchi, R. (2009). Cuticular hydrocarbons in the stingless bee Schwarziana quadripunctata (Hymenoptera, Apidae, Meliponini): differences between colonies, castes and age. Genetics and Molecular Research, 8: 589-595.

O’Donnell, S. & Jeanne, R. L. (1992). The effects of colony characteristics on life, span and foraging behavior of individual wasps (Polybia occidentalis, Hymenoptera: Vespidae). Insect Sociaux 39: 73-80. doi:10.1007/BF01240532

Panek, L. M., Gamboa, G. J., Espelie, K. E. (2001). The effect of a wasp’s age on its cuticular hydrocarbon profile and its tolerance by nestmate and non-nestmate conspecifics (Polistes fuscatus, Hymenoptera: Vespidae). Ethology, 107: 55-63. doi: 10.1046/j.1439-0310.2001.00633.x

Quinn, G. P. & Keough, M. J. (2002). Experimental design and data analysis for biologists. Cambridge University Press, Cambridge.

Richard, F. J., Hefetz, A., Christides, J. P., Errard, C. (2004). Food influence on colonial recognition and chemical signature between nestmates in the fungus-growing ant Acromyrmex subterraneus subterraneus. Chemoecology, 16: 9-16. doi:10.1007/s00049-003-0251-3

Ridley, M. (2006). Evolução. In Ridley, M. (Eds.). Conceitos de espécies e variação intra-específica (pp 375-406). 3a edição. Porto Alegre: Armed.

Ross, R. W. & Matthews, R. W. (1991). The social biology of wasps. Department of Entomology: University of Georgia.

Ruther, J., Sieben, S. & Schricker, B. (2002). Nestmate recognition in social wasps: manipulation of hydrocarbon profiles induces aggression in the European hornet. Naturwissenschaften, 89: 111–114. doi:10.1007/s00114-001-0292-9

Shellman, J. S. & Gamboa, G. J. (1982). Nestmate discrimination in social wasps: The role of exposure to nest and nestmates (Polistes fuscatus, Hymenoptera: Vespidae). Behavioral Ecology and Sociobiology, 11: 51-53. doi:10.1007/BF00297666

Singer TL, Camann MA, Espelie KE (1992). Discriminant analysis of cuticular hydrocarbons of social wasp Polistes exclamans Viereck and nest surface hydrocarbons of its nest paper and pedicel. Journal Chemical Ecology, 18: 785-797. doi:10.1007/BF00994615

Singer, T. L. & Espelie, K. E. (1996). Nest surface hydrocarbons facilitate nestmate recognition for the social wasp, Polistes metricus Say (Hymenoptera: Vespidae). Journal of Insect Behavior, 9: 857-870. doi:10.1007/BF02208974

Singer, T. L., Espelie, K. E. & Gamboa, G. J. (1998). Nest and nestmate discrimination in independent-founding wasps. In Vander Meer, R. K., Breed, M. D., Winston, M. L. & Espelie, E. K. (Eds.) Pheromone communication in social insects (pp 104-125). Boulder: Westview.

Sledge, M. F., Boscaro, F. & Turillazzi, S. (2001). Cuticular hydrocarbons and reproductive status in the social wasp Polistes dominulus. Behavioral Ecology and Sociobiology, 49: 401-409.

Sorvari, J., Theodora, P., Turillazzi, S., Hakkarainen, H. & Sundsteöm, L. (2008). Food resources, chemical signaling, and nestmate recognition in the ant Formica aquilonia. Behavioral Ecology, 19: 441-447. doi:10.1093/beheco/arm160

Tannure-Nascimento, I. C., Nascimento, F. S., Turatti, I. C., Lopes, N. P., Trigo, J. R., & Zucchi, R. (2007). Colony membership is reflected by variations in cuticular hydrocarbon profile in a Neotropical paper wasp, Polistes satan (Hymenoptera, Vespidae). Genetics and Molecular Research, 6: 390-396.

Torres, V. O., Montagna, T. S., Raizer, J. & Antonialli-Junior, W. F. (2012). Division of labor in colonies of the eusocial wasp, Mischocyttarus consimilis Journal of Insect Science, 12: 1-15. doi:10.1673/031.012.2101

Van Zweden, J. S., Bonckaert, W., Wenseleers, T., & D'Ettorre, P. (2013). Queen signaling in social wasps. Evolution, 68: 976-986. doi:10.1111/evo.12314

Wagner, D., Tissot, M., & Gordon, D. (2001). Task-related environment alters the cuticular hydrocarbon composition of harvester ants. Journal of Chemical Ecology, 27: 1805-1819. doi:10.1023/A:1010408725464

Zweden, J. S. V., Dreier, S. & D’Ettorre, P. (2009). Disentangling environmental and heritable nestmate recognition cues in a carpenter ant. Journal of Insect Physiology, 55: 158-163. doi:10.1016/j.jinsphys.2008.11.001




How to Cite

Ferreira, A. C., Neves, E. F., Montagna, T. dos S., Mendonça, A., Cardoso, C. A. L., & Antonialli Junior, W. F. (2017). Intraspecific Variation of the Composition of Linear Alkanes in Social Wasp Mischocyttarus consimilis. Sociobiology, 64(4), 466–476.



Research Article - Wasps

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