Insecticidal Activity of the Leaf and Stem Water Extract of Gelsemium elegans against Solenopsis invicta


  • Qun Zheng South China Agricultural University
  • Liu-Peng Yang South China Agricultural University
  • Su-Kun Lin South China Agricultural University
  • Qian-Li Ma South China Agricultural University
  • De-Qiang Qin South China Agricultural University
  • Zhi-Xiang Zhang South China Agricultural University



Gelsemium elegans Benth.,koumine,worker ant,bioactivity


A comprehensive green worker ants control method that can be used to replace traditional chemical synthetic insecticides. In this study, the leaves and stems of Gelsemium elegans were extracted with water as the solvent, and the bioactivity of G. elegans against worker ants was determined by the “water tube” method. The bioassay results of insecticidal activity showed that when the time was extended to the 10th day, the mortality of worker ants treated with G. elegans extract reached 55.00% (1/20 leaf extract), 46.67% (1/20 stem extract) and 45.00% (1 mg/kg koumine). And the behavioral impact test results showed that the aggregation rate was reduced to 56.67% (1/100 leaf extract), 60.00% (1/100 stem extract) and 60.00% (0.5 mg/kg koumine); the climbing rate was reduced to 60.00 % (1/100 leaf extract), 58.33% (1/100 stem extract) and 58.33% (0.5 mg/kg koumine). The effect on the walking ability of worker ants is obvious. The walking rate drops to 1.53cm/s (1/100 leaf extract), 1.60cm/s (1/100 stem extract) and 1.47cm/s (0.5 mg/kg koumine). Therefore, we conclude that the water extract of G. elegans can be used for long-term continuous control of worker ants, which can be used to replace traditional chemical synthetic insecticides.


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Appel, A. G., Gehret, M. J., & Tanley, M. J. (2004). Repellency and toxicity of mint oil granules to red imported fire ants (Hymenoptera : Formicidae). Journal of Economic Entomology, 97(2), 575-580. doi: 10.1603/0022-0493-97.2.575

Ascunce, M. S., Yang, C., Oakey, J., Calcaterra, L., Wu, W., Shih, C.,... Shoemaker, D. (2011). Global Invasion History of the Fire Ant Solenopsis invicta. Science, 331(6020), 1066-1068. doi: 10.1126/science.1198734

Chen, J. (2009). Repellency of an Over-the-Counter Essential Oil Product in China against Workers of Red Imported Fire Ants. Journal of Agricultural and Food Chemistry, 57(2), 618-622. doi: 10.1021/jf8028072

Cheng, S., Lin, J., Lin, C., Hsui, Y., Lu, M., Wu, W.,... Chang, S. (2008). Terminating red imported fire ants using Cinnamomum osmophloeum leaf essential oil. Bioresource Technology, 99(4), 889-893. doi: 10.1016/j.biortech.2007.01.039

Hu, W., Zhang, N., Chen, H., Zhong, B., Yang, A., Kuang, F.,... Chun, J. (2017). Fumigant Activity of Sweet Orange Essential Oil Fractions Against Red Imported Fire Ants (Hymenoptera: Formicidae). Journal of Economic Entomology, 110(4), 1556-1562. doi: 10.1093/jee/tox120

Huang, C. L., Fu, J. T., Liu, Y. K., Cheng, D. M., & Zhang, Z. X. (2015). The Insecticidal and Repellent Activity of Soil Containing Cinnamon Leaf debris against Red Imported Fire Ant Workers. Sociobiology, 62(1), 46-51

Huang, R. L., Li, Z. H., Wang, S. Y., Fu, J. T., Cheng, D. M.,... Zhang, Z. X. (2016). Insecticidal effect of volatile compounds from plant materials of Murraya exotica against Red Imported Fire Ant Workers. Sociobiology, 63(2), 783-791. doi: 10.13102/sociobiology.v63i2.972

Huang, S. Q., Fu, J. T., Wang, K., Xu, H. H., & Zhang, Z. X. (2016). Insecticidal activity of the methanol extract of Pronephrium megacuspe (Thelypteridaceae) and its active component on Solenopsis invicta (Hymenoptera: Formicidae). Florida Entomologist, 99(4), 634-638. doi: 10.1653/024.099.0408

Kafle, L., & Shih, C. J. (2013). Toxicity and Repellency of Compounds From Clove (Syzygium aromaticum) to Red Imported Fire Ants Solenopsis invicta (Hymenoptera: Formicidae). Journal of Economic Entomology, 106(1), 131-135. doi: 10.1603/EC12230

LeBrun, E. G., Abbott, J., & Gilbert, L. E. (2013). Imported crazy ant displaces imported fire ant, reduces and homogenizes grassland ant and arthropod assemblages. Biological Invasions, 15(11), 2429-2442. doi: 10.1007/s10530-013-0463-6

Liu, M., Huang, H., Yang, J., Su, Y., Lin, H., Lin, L.,... Yu, C. (2013). The active alkaloids of Gelsemium elegans Benth. are potent anxiolytics. Psychopharmacology, 225(4), 839-851. doi: 10.1007/s00213-012-2867-x

Qin, D. Q., Huang, R. L., Li, Z. H., Wang, S. Y., Cheng, D. M.,... Zhang, Z. X. (2018). Volatile Component Analysis of Michelia alba Leaves and Their Effect on Fumigation Activity and Worker Behavior of Solenopsis invicta. Sociobiology, 65(2), 170-176. doi: 10.13102/sociobiology.v65i2.2014

Souto, R. N. P., Harada, A. Y., Andrade, E. H. A., & Maia, J. G. S. (2012). Insecticidal Activity of Piper Essential Oils from the Amazon Against the Fire Ant Solenopsis saevissima (Smith) (Hymenoptera: Formicidae). Neotropical Entomology, 41(6), 510-517. doi: 10.1007/s13744-012-0080-6

Tang, L., Sun, Y. Y., Zhang, Q. P., Zhou, Y., Zhang, N.,... Zhang, Z. X. (2013). Fumigant Activity of Eight Plant Essential Oils Against Workers of Red Imported Fire Ant, Solenopsis invicta. Sociobiology, 60(1), 35-40.doi:10.13102/sociobiology.v60i1.35-40

Vogt, J. T., Shelton, T. G., Merchant, M. E., Russell, S. A., Tanley, M. J.,... Appel, A. G. (2002). Efficacy of three citrus oil formulations against Solenopsis invicta Buren (Hymenoptera : Formicidae), the red imported fire ant. Journal of Agricultural and Urban Entomology, 19(3), 159-171

Wetterer, J. K. (2011). Worldwide spread of the tropical fire ant, Solenopsis geminata (Hymenoptera: Formicidae). Myrmecological News, 14, 21-35

Zhang, Y., Fu, J. T., Huang, C. L., Cheng, D. M., Huang, R. L.,... Zhang, Z. X. (2017). Insecticidal Activity of the Soil in the Rhizosphere of Viburnum odoratissimum against Solenopsis invicta (Hymenoptera: Formicidae). Sociobiology, 64(1), 1-6. doi: 10.13102/sociobiology.v64i1.1067

Zhang, Z. X., Zhou, Y., & Cheng, D. M. (2013). Effects of Hematoporphyrin Monomethyl Ether on Worker Behavior of Red Imported Fire Ant Solenopsis invicta. Sociobiology, 60(2), 169-173.doi:10.13102/sociobiology.v60i2.169-173




How to Cite

Zheng, Q., Yang, L.-P., Lin, S.-K., Ma, Q.-L., Qin, D.-Q., & Zhang, Z.-X. (2020). Insecticidal Activity of the Leaf and Stem Water Extract of Gelsemium elegans against Solenopsis invicta. Sociobiology, 67(2), 232–238.



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