Chromosome Mapping of Ribosomal DNA Clusters in Four Leaf-cutting Ant Species of the Genus Acromyrmex, with Description of a Triploid Individual in A. laticeps

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DOI:

https://doi.org/10.13102/sociobiology.v71i3.10403

Keywords:

Cytogenetics, fungus-growing ants, polyploidy, rDNA, FISH, Formicidae

Abstract

The increase of cytogenetic data in the leaf-cutting genus Acromyrmex has shown interesting contributions to the evolutionary and taxonomic approaches, including the creation of a new genus and the description of chromosomal patterns allowing comparative studies within Acromyrmex as well as between this genus and other similar taxa. The conserved chromosome number in Acromyrmex is a notable feature, with some distinguishable chromosomes among the species. In this study, we describe the diploid karyotype of Acromyrmex laticeps in addition to an individual with all the metaphases triploids, suggesting a triploid individual. To the best of our knowledge, this is the first report of polyploidy in a fungus-growing ant. Furthermore, we mapped 18S ribosomal DNA on the chromosomes of A. laticeps and three other Acromyrmex species. Among them, three species showed the ribosomal DNA cluster pattern observed in other previously studied members of this genus, with a terminal location in the largest subtelocentric pair. Meanwhile, Acromyrmex balzani had these ribosomal DNA clusters in its entire short arm. The role of triploidy in ant evolution has also been discussed. The increase in chromosomal data of leaf-cutting ants may provide significant insights into the evolution of this peculiar ant group.

 

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References

Barros, L.A.C., Teixeira, G.A., Aguiar, H.J.A.C., Mariano, C.S.F, Delabie, J.H.C., Pompolo, S.D.G. (2014). Banding patterns of three leafcutter ant species of the genus Atta (Formicidae: Myrmicinae) and chromosomal inferences. Florida Entomologist, 1694-1701. https://doi.org/10.1653/024.097.0444. DOI: https://doi.org/10.1653/024.097.0444

Barros, L.A.C., Aguiar, H.J.A.C., Mariano, C.S.F., Andrade-Souza, V., Costa, M.A., Delabie, J.H.C., Pompolo, S.G. (2016). Cytogenetic data on six leafcutter ants of the genus

Acromyrmex Mayr, 1865 (Hymenoptera, Formicidae, Myrmicinae): insights into chromosome evolution and taxonomic implications. Comparative Cytogenetics, 10: 229. https://doi.org/10.3897/CompCytogen.v10i2.7612. DOI: https://doi.org/10.3897/CompCytogen.v10i2.7612

Barros, L.A.C., Aguiar, H.J.A.C., Teixeira, G.A., Souza, D.J., Delabie, J.H.C., Mariano, C.S.F. (2021). Cytogenetic studies on the social parasite Acromyrmex ameliae (Formicidae: Myrmicinae: Attini) and its hosts reveal chromosome fusion in Acromyrmex. Zoologischer Anzeiger, 293: 273-281. https://doi.org/10.1016/j.jcz.2021.06.012. DOI: https://doi.org/10.1016/j.jcz.2021.06.012

Bolton, B., (2023). An online catalog of the ants of the world. Available from: https://antcat.org. (Accessed 26 October 2023).

Cardoso, D.C., Cristiano, M.P. (2021). Karyotype diversity, mode, and tempo of the chromosomal evolution of Attina (Formicidae: Myrmicinae: Attini): Is there an upper limit to chromosome number? Insects, 12: 1084. https://doi.org/10.3390/insects12121084. DOI: https://doi.org/10.3390/insects12121084

Cristiano, M.P., Cardoso, D.C., Fernandes-Salomão, T.M. (2013). Cytogenetic and molecular analyses reveal a divergence between Acromyrmex striatus (Roger, 1863) and other congeneric species: taxonomic implications. PLoS One, 8: e59784. https://doi.org/10.1371/journal.pone.0059784. DOI: https://doi.org/10.1371/journal.pone.0059784

Cristiano, M.P., Cardoso, D.C., Sandoval-Gómez, V.E., Simões-Gomes, F.C. (2020). Amoimyrmex Cristiano, Cardoso & Sandoval, gen. nov. (Hymenoptera: Formicidae): a new genus of leaf-cutting ants revealed by multilocus molecular phylogenetic and morphological analyses. Austral Entomology, 59: 643-676. https://doi.org/10.1111/aen.12493. DOI: https://doi.org/10.1111/aen.12493

Crozier, R.H. (1975). Animal cytogenetics. Ed. by: John, B., Bauer, H., Brown, S., Kayano, H., Levan, A., White, M. Gebrüder Borntraeger, Berlin. 94 pp.

De Andrade, A.R.M., Cardoso, D.C., Cristiano, M.P. (2023). Assessing ploidy levels and karyotype structure of the fire ant Solenopsis saevissima Smith, 1855 (Hymenoptera, Formicidae, Myrmicinae). Comparative Cytogenetics, 17: 59-73. https://doi.org/10.3897/compcytogen.17.100945. DOI: https://doi.org/10.3897/compcytogen.17.100945

Forti, L.C., Andrade, A.P.P., Camargo, R.S., Mota-Filho, T.M.M., Scudillio, T.T., Zanuncio, J.C. et al. (2022). Taxonomic revision of the leaf-cutting ant Acromyrmex subterraneus (Forel, 1893). International Journal of Tropical Insect Science, 42: 2897-2908. https://doi.org/10.1007/s42690-022-00815-5. DOI: https://doi.org/10.1007/s42690-022-00815-5

Gokhman, V.E. (2023). Chromosome Study of the Hymenoptera: History, Current State, Perspectives. Biology Bulletin Reviews, 13: 247-257. https://doi.org/10.1134/S2079086423030040. DOI: https://doi.org/10.1134/S2079086423030040

Gokhman, V.E., Kuznetsova, V.G. (2017). Parthenogenesis in Hexapoda: holometabolous insects. Journal of Zoological Systematics and Evolutionary Research, 56: 23-34. https://doi.org/10.1111/jzs.12183. DOI: https://doi.org/10.1111/jzs.12183

Imai, H.T., Crozier, R.H., Taylor, R.W. (1977). Karyotype evolution in Australian ants. Chromosoma, 59: 341-393. DOI: https://doi.org/10.1007/BF00327974

Imai, H.T., Taylor, R.W., Crosland, M.W., Crozier, R.H., (1988). Modes of spontaneous chromosomal mutation and karyotype evolution in ants with reference to the minimum interaction hypothesis. The Japanese Journal of Genetics, 63: 159-185. https://doi.org/10.1266/jjg.63.159. DOI: https://doi.org/10.1266/jjg.63.159

Krieger, M.J., Ross, K.G., Chang, C.W., Keller, L. (1999). Frequency and origin of triploidy in the fire ant Solenopsis invicta. Heredity, 82: 142-150. https://doi.org/10.1046/j.1365-2540.1999.00460.x. DOI: https://doi.org/10.1038/sj.hdy.6884600

Levan, A., Fredga, K., Sandberg, A.A. (1964). Nomenclature for centromeric position on chromosomes. Hereditas, 52: 201-220. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x. DOI: https://doi.org/10.1111/j.1601-5223.1964.tb01953.x

Lorite, P., Palomeque, T. (2010). Karyotype evolution in ants (Hymenoptera: Formicidae), with a review of the known ant chromosome numbers. Myrmecological News, 13: 89-102.

Mariano, C.S.F., Santos, I.S., Silva, J.G., Costa, M.A., Pompolo, S.G. (2015). Citogenética e evolução do cariótipo em formigas poneromorfas. In: Delabie, J.H.C., Feitosa, R.M., Serrao, J.E., Mariano, C.S.F., Majer, J.D. (Eds.) As formigas poneromorfas do Brasil, 1st edn. Ilhéus, Brasil, pp 102-125. DOI: https://doi.org/10.7476/9788574554419.0010

Mariano, C.S.F., Barros, L.A.C., Velasco, Y.M., Guimarães, I.N., Pompolo, S.G., Delabie, J.H.C. (2019). Citogenética de hormigas de la región neotropical. In: Fernández, F., Guerrero, R., Delsinne, T. (Eds.) Hormigas de Colombia. Universidad Nacional de Colombia, Bogotá, Colombia, pp 131-157.

Mayhé-Nunes, A.J., Jaffé, K. (1998). On the biogeography of Attini (Hymenoptera: Formicidae). Ecotropicos, 11: 45-54.

Micolino, R., Baldez, B.C.L., Sánchez-Restrepo, A.F., Calcaterra, L., Cristiano, M.P., Cardoso, D.C. (2022). Karyotype structure and cytogenetic markers of Amoimyrmex bruchi and Amoimyrmex silvestrii: contribution to understanding leaf-cutting ant relationships. Genome, 65: 43-51. https://doi.org/10.1139/gen-2021-0044. DOI: https://doi.org/10.1139/gen-2021-0044

Murakami, T., Paris, C., Chirino, M., Sasa, C., Sakamoto, H., Higashi, S., Sato, K. (2021). Unusual chromosome numbers and polyploidy in invasive fire ant populations. Genetica, 149: 203-215. https://doi.org/10.1007/s10709-021-00128-4. DOI: https://doi.org/10.1007/s10709-021-00128-4

Otto, S.P., Whitton, J. (2000). Polyploid incidence and evolution. Annual Review of Genetics, 34: 401-437. https://doi.org/10.1146/annurev.genet.34.1.401. DOI: https://doi.org/10.1146/annurev.genet.34.1.401

Pereira, J.O.P. (2006). Diversidade genética da abelha sem ferrão Melipona quinquefasciata baseada no sequenciamento das regiões ITS1 parcial e 18S do DNA ribossômico nuclear. D.Sc. Thesis, Programa de Pós-graduação em Zootecnia. Universidade Federal do Ceará, Fortaleza-CE, Brazil. 141 pp.

Pinkel, D., Straume, T., Gray, J.W. (1986). Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proceedings of the National Academy of Sciences, 83: 2934-2938. https://doi.org/10.1073/pnas.83.9.2934. DOI: https://doi.org/10.1073/pnas.83.9.2934

Schultz, T.R. (2021). Fungus-Farming Ants (Attini in Part). Encyclopedia of Social Insects, 404-411. DOI: https://doi.org/10.1007/978-3-030-28102-1_46

Schweizer, D. (1980). Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA/DAPI-bands) in human chromosomes. Cytogenetics and Cell Genetics, 27: 190-193. https://doi.org/10.1159/000131482. DOI: https://doi.org/10.1159/000131482

Silva, A.P.A. (2016). Cytogenetic Description of 13 morpho-species of Solenopsis Westwood, 1840 (Hymenoptera: Formicidae). Ph.D. Dissertation, Universidade Federal de Viçosa, 56 pp.

Symonová, R. (2019). Integrative rDNAomics – Importance of the oldest repetitive fraction of the eukaryote genome. Genes, 10: 345-360. https://doi.org/10.3390/genes10050345. DOI: https://doi.org/10.3390/genes10050345

Teixeira, G.A., Barros, L.A.C., Aguiar, H.J.A.C., Pompolo, S.G. (2017). Comparative physical mapping of 18S rDNA in the karyotypes of six leafcutter ant species of the genera Atta and Acromyrmex (Formicidae: Myrmicinae). Genetica, 145: 351-357. https://doi.org/10.1007/s10709-017-9970-1. DOI: https://doi.org/10.1007/s10709-017-9970-1

Teixeira, G.A., Aguiar, H.J.A.C., Petitclerc, F., Orivel, J., Lopes, D.M., Barros, L.A.C. (2021a). Evolutionary insights into the genomic organization of major ribosomal DNA in ant chromosomes. Insect Molecular Biology, 30: 340-354. https://doi.org/10.1111/imb.12699. DOI: https://doi.org/10.1111/imb.12699

Teixeira, G.A., Barros, L.A.C., Aguiar, H.J.A. C., Lopes, D.M. (2021b). Distribution of GC-rich heterochromatin and ribosomal genes in three fungus-farming ants (Myrmicinae, Attini, Attina): insights on chromosomal evolution. Comparative Cytogenetics, 15: 413. https://doi.org/10.3897/compcytogen.v15.i4.73769. DOI: https://doi.org/10.3897/compcytogen.v15.i4.73769

Teixeira, G.A., Barros, L.A.C., Silveira, L.I., Orivel, J., Lopes, D.M., Aguiar, H.J.A.C. (2022). Karyotype conservation and genomic organization of repetitive sequences in the leaf-cutting ant Atta cephalotes (Linnaeus, 1758) (Formicidae: Myrmicinae). Genome, 65: 525-535. https://doi.org/10.1139/gen-2021-0129. DOI: https://doi.org/10.1139/gen-2021-0129

Toda, E., Okamoto, T. (2016). Formation of triploid plants via possible polyspermy. Plant Signaling and Behavior, 11: e1218107. https://doi.org/10.1080/15592324.2016.1218107. DOI: https://doi.org/10.1080/15592324.2016.1218107

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Published

2024-07-25

How to Cite

Barros, L. A. C., Teixeira, G. A., Lod, R. B., Arnhold, A., & de Aguiar, H. J. A. C. (2024). Chromosome Mapping of Ribosomal DNA Clusters in Four Leaf-cutting Ant Species of the Genus Acromyrmex, with Description of a Triploid Individual in A. laticeps. Sociobiology, 71(3), e10403. https://doi.org/10.13102/sociobiology.v71i3.10403

Issue

Vol. 71 No. 3 (2024)

Section

Research Article - Ants

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Copyright (c) 2024 Luísa Antonia Campos Barros, Gisele Amaro Teixeira, Rodrigo Batista Lod, Alexandre Arnhold, Hilton Jeferson Alves Cardoso de Aguiar

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