Enzyme stabilization: An approach
DOI:
https://doi.org/10.13102/scb7962Abstract
Enzyme stabilization is one critic point in basic and applied enzymology. The increasing interest in applying enzymes in industrial processes has fostered the search for biocatalysts with new properties or extreme stability. Enzyme stabilization can be achieved by different methods: isolating enzyme variants from organisms living in appropriate extreme environments (extremozymes), by protein engineering, chemical modification, use of additives, immobilization. This brief review aims to give a better understanding of those methods employed for enzyme stabilization.
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ANDERSSON MM & R HATTI-KAUL. 1999. Protein stabilising effect of polyethyleneimine. J. Biotech. 72: 21-31.
BIENIARZ C, MJ CORNWELL & DF YOUNG. 1998. Alkaline phosphatase activatable polymeric cross-linkers and their use in the stabilization of proteins. Bioconjugate Chem. 9:390-8.
BILATI U, E ALLÉMANN & E DOELKER. 2005. Strategic approaches for overcoming peptide and protein instability within biodegradable nano- and microparticles. European J. Pharmaceutics and Biopharmaceutics 59: 375-388.
BRUGGER R, A KRONENBERGER, A BISCHOFF, D HUG, M LEHMANN, APGM VAN LOON & M MARKUS WYSS. 2001. Thermostability engineering of fungal phytases using low-Mr additives and chemical crosslinking. Biocatalysis and Biotransformation 19(5-6): 505-516.
CAMPOS LA, MM GARCIA-MIRA, R GODOY-RUIZ, JM SANCHEZ-RUIZAND & J SANCHO. 2004. Do proteins always benefit from a stability increase? Relevant and residual stabilization in a three-state. protein by charge optimization. J. Mol. Biol. 344: 223-237.
CASTRO HF, PC OLIVEIRA & CMF SOARES. 1997. Parâmetros reacionais para a síntese enzimática do butirato de butila em solventes orgânicos. Ciênc. Tecnol. Aliment. 17: 237-241.
CHIBATA I. 1972. Characteristics of immobilized aminoacylase from Aspergillus oryzae on macroporous copolymers, p. 383-389. Fermentation technology today. Japan: G. Terni Ltda.
COSTA SA, T TZANOV, AF CARNEIRO, A PAAR, GM GÜBITZ & A CAVACO-PAULO. 2002. Studies of stabilization of native catalase using additives. Enzyme and Microbial Technology 30: 387-391.
COWAN DA. 1997. Thermophilic proteins: stability and function in aqueous and organic solvent. Comp. Biochem. Physiol. 118: 429-438.
DALLA-VECCHIA R, MG NASCIMENTO & V VOLDI. 2004. Aplicações sintéticas de lipases imobilizadas em polímeros. Química Nova 27: 623-630.
DAVIS GC. 1993. Protein stability: impact upon protein pharmaceuticals. Biologicals 21: 105.
EIJSINK VGH, BJørk A, GÅSEIDNES S, SIREVAG R, BORCHERT TV, VAN DEN BURG B, 2005. Directed evolution of enzyme stability. Biomolecular Engineering 22: 21-30.
HAKI GD & SK RAKSHIT. 2003. Developments in industrially important thermostable enzymes: a review. Bioresource Technology 89: 17-34.
GIANFREDA L & MR SCARFI. 1991. Enzyme stabilization: state of the art. Mol. Cell. Biochem. 100: 97-128.
GOVARDHAN CP. 1999. Crosslinking of enzymes for improved stability and performance. Curr. Opin. Biotechnol. 10: 331-335.
GRUBHOFER N & L SCHLEITH. 1953. Modified ion exchangers stationary phases in chromatography. Naturwissenschaften 40: 508.
HIBBERT EG & PA DALBY. 2005 Directed evolution strategies for improved enzymatic performance. Microbial Cell Factories 4:29
HORIKOSHI K. 1999. Alkaliphiles: some applications of their products for biotechnology. Microbiol. Mol. Biol. Rev. 63: 735-750.
HOUGH DW & M DANSON. 1999. Extremozymes. Current Opin. in Chem. Biol. 3:39-46.
ILLANES A. 1999. Stability of biocatalysts. Electronic J. Biotech. 2(1), issue of April 15.
JONES AJS & JL CLELAND. 1996. Technical and regulatory hurdles in delivery aspects of macromolecular drugs. Control Release 41: 147-155.
KENNEDY JF & JMS CABRAL. 1987. Enzyme immobilization, p. 347-406. In: HJ REHM & G REED (Eds.). Enzyme technology. Weinhem: VCH.
KIM J, JW GRATE & P WANG. 2006. Nanostructures for enzyme stabilization. Chemical Engineering Science 61: 1017-1026.
LEHNINGER AL, DL NELSON & MM COX. 1995. Princípios de bioquímica. 2a ed. São Paulo: Sarvier.
LIMA AWO & ANGNES L. 1999. Biocatalysis in aquo-restricted media: fundamentals and applications in analytical chemistry. Química Nova 22: 229-245.
MARCOZZI G, C DI DOMENICO & N SPRETI. 1998. Biotech. Prog. 14: 653-656.
MATSUSHIMA A, Y KODERA & M HIROTO. 1996. Bioconjugates of proteins and polyethylene glycol; potent tools in biotechnological processes J. Mol. Catal. B, 2: 1.
MATTHEWS BW, H NICHOLSON & WJ BECKTEL. 1987. Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding. Proc. Natl. Acad. Sci. 84: 6663-6667.
MONSAN P, D COMBES & I ALEMZAHED. 1984. Invertase covalent grafting onto corn stover. Biotechnol. Bioeng. 26: 658-664.
MOZHAEV VV, NS MELIK-NUBAROV, MV SERGEEVA, V SIKSNIS & K MARTINEK. 1990. Strategy for stabilizing enzymes. Part one: increasing stability of enzymes via their multi-point interaction with a support. Biocatalysis 3: 179-187.
O’FAGAIN C. 2003. Enzyme stabilization-recent experimental progress. Enzyme and Microbial Tech. 33: 137-149.
RAMAKRISHNAN V, MFJM VERHAGEN & MWW ADAMS. 1997. Characterization of di-myo-inositol-1,1%-phosphate in the hyperthermophilic bacterium Thermotoga maritina. Appl. Environ. Microbiol. 63: 347-350.
SILMAR IH & E KATCHALSKI. 1966. Water-insoluble derivatives of enzymes antigens and antibodies. Annu. Rev. Biochem. 35: 873-908.
SNIR R, PE KOEHLER, KA SIMS & L WICKER. 1995. pH and cations influence permeability of marsh grapefruit pectinesterase on polysulfone ultrafiltration membrane. J. Agric. Food Chem. 43: 1157-1161.
SOARES CMF, MHA SANTANA, GM ZANIN & HF CASTRO. 2003. Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico. Química Nova 26: 832-838.
SUMNER JB. 1948. Denaturation of urease without inactivation. Science 108: 410.
VAN DEN BURG, B. 1999. Extremophiles as a source for novel enzymes. Current Opinion in Microbiol. 6: 213-218.
VAZOLLER RF, GP MANFIO & VP CANHOS. 1999. Biodiversidade do Estado de São Paulo, Brasil: síntese do conhecimento ao final do século XX. In: CA JOLY & CEM BICUDO (Orgs.). Microrganismos & Vírus. São Paulo: FAPESP.
VICENTE AA. 2000. Preparação de açúcar invertido por meio de invertase imobilizada em sílica. Dissertação (Mestrado em Biotecnologia) - Instituto de Química, Universidade Estadual Paulista, Araraquara.
VIEILLE C & JG ZEIKUS. 1996. Thermoenzymes: identifying molecular determinants of protein structural and functional stability. Trends Biotechnol. 14: 183-191.
VIGUERA AR, JC MARTINEZ, VV FILIMONOV, PL MATEO & L SERRANO. 1993. Thermodynamic and kinetic analysis of the SH3 domain of spectrin shows a two-state folding transition Biochemistry 33: 2142-2150.
VISURI K, O PASTINEN, X WU, K MÄKINEN & M LEISOLA. 1999. Stability of native and cross-linked crystalline glucose isomerise. Biotechnol. Bioeng. 64:377-80.
WANG J, JIE LIU & G CEPRA. 1997. Thermal stabilization of enzymes immobilized within carbon paste electrodes. Anal. Chem. 69: 3124-3127.
WANG W. 1999. Instability, stabilization, and formulation of liquid protein pharmaceuticals. Intern. J. Pharmaceutics 185: 129-188.
WINGARD JR. 1972. Enzyme engineering: a new area of specialization. Biotechnol. Bioeng. Symp. 3: 3-13.
WUNDERLICH M, A MARTIN & CA STAAB. 2005. J. Mol. Biol. 351(5): 1160-1168.
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